The DOI matches its Crossref record; 45 of the 46 references it cites were found in a registry or an index.
Oxidized Phospholipids Increase Interleukin 8 (IL-8) Synthesis by Activation of the c-src/Signal Transducers and Activators of Transcription (STAT)3 Pathway
Open accessAbstract
Oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (Ox-PAPC) and its component phospholipids 1-palmitoyl-2-epoxyisoprostane-sn-glycero-3-phosphorylcholine (PEIPC) and 1-palmitoyl-2-(5-oxovaleroyl)-sn-glycero-3-phosphorylcholine induce endothelial cells to synthesize chemotactic factors, such as interleukin 8 (IL-8). We have shown recently that Ox-PAPC-mediated induction of IL-8 transcription is independent of NF-kappaB activation, a major transcription factor utilized by cytokines and lipopolysaccharide for the induction of IL-8 transcription. In this study, we provide evidence for the role of c-src in Ox-PAPC and, specifically, PEIPC-mediated IL-8 induction. Ox-PAPC and its component phospholipids induced a rapid and transient phosphorylation of c-src Tyr418, a hallmark of c-src activation, in human aortic endothelial cells (HAEC). Ox-PAPC-mediated IL-8 protein synthesis in HAEC was inhibited by Src family kinase inhibitors, PP1 and PP2, but not by an inactive analog, PP3. Transient expression of plasmids containing C-terminal Src kinase or kinase-deficient dominant-negative c-src resulted in a 72 and 50% reduction in Ox-PAPC-induced IL-8 promoter activation in human microvascular endothelial cells, respectively. In contrast, overexpression of v-src kinase resulted in a 4-fold increase in IL-8 promoter activation, without inducing NF-kappaB promoter activation. Furthermore, treatment of HAEC with Ox-PAPC and its component PEIPC induced the activation of STAT3 by phosphorylating Tyr705, a feature of STAT3 activation. STAT3 is a known downstream effector of c-src. Ox-PAPC-induced activation of STAT3 resulted in the translocation of STAT3 from the cytoplasm of HAEC into their nuclear compartment. Transient expression of a dominant-negative STAT3beta construct in HMEC strongly inhibited IL-8 induction by Ox-PAPC. Taken together, these data demonstrate the role of the c-src kinase/STAT3 pathway in Ox-PAPC-mediated IL-8 expression in endothelial cells.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“spliceform-specific functions have been indicated by various in vivo studies showing a requirement for STAT3β during endotoxic assault [16], but a requirement for STAT3α in IL-8 synthesis [17], as well as differential roles for STAT3α and STAT3β in anti-inflammatory responses [15].”
From Selective STAT3-α or -β expression reveals spliceform-specific phosphorylation kinetics, nuclear retention and distinct gene expression outcomes (2012) · cites this paper as [17]
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Dignam, J. D., Lebovitz, R. M., & Roeder, R. G. (1983) · Nucleic Acids Research · doi:10.1093/nar/11.5.1475 · Cited by 11,262
Darnell, J. E., Kerr, L. M., & Stark, G. R. (1994) · Science · doi:10.1126/science.8197455 · Cited by 6,080
Lusis, A. J. (2000) · Nature · doi:10.1038/35025203 · Cited by 4,880
Darnell, J. E. (1997) · Science · doi:10.1126/science.277.5332.1630 · Cited by 3,979
Catlett-Falcone, R., Landowski, T. H., Oshiro, M. M., Turkson, J., Levitzki, A., Savino, R., Ciliberto, G., Moscinski, L., Fernández-Luna, J. L., Nuñez, G., et al. (1999) · Immunity · doi:10.1016/s1074-7613(00)80011-4 · Cited by 1,638
Traub, O., & Berk, B. C. (1998) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.18.5.677 · Cited by 1,079
Toll-like Receptor 4 Polymorphisms and Atherogenesis
Kiechl, S. J., Lorenz, E., Reindl, M., Wiedermann, C. J., Oberhollenzer, F., Bonora, E., Willeit, J., & Schwartz, D. A. (2002) · New England Journal of Medicine · doi:10.1056/nejmoa012673 · Cited by 1,022
Enhanced DNA-Binding Activity of a Stat3-Related Protein in Cells Transformed by the Src Oncoprotein
Yu, C.-L., Meyer, D. J., Campbell, G. S., Larner, A. C., Carter-Su, C., Schwartz, J., & Jove, R. (1995) · Science · doi:10.1126/science.7541555 · Cited by 931
Martin, G. S. (2001) · Nature Reviews Molecular Cell Biology · doi:10.1038/35073094 · Cited by 615
Activation and Association of Stat3 with Src in v-Src-Transformed Cell Lines
Cao, X., Tay, A., Guy, G. R., & Tan, Y. H. (1996) · Molecular and Cellular Biology · doi:10.1128/mcb.16.4.1595 · Cited by 359
Sieg, D. J., Ilić, D., Jones, K. C., Damsky, C. H., Hunter, T., & Schlaepfer, D. D. (1998) · The EMBO Journal · doi:10.1093/emboj/17.20.5933 · Cited by 335
v-SRC'S hold over actin and cell adhesions
Frame, M. C., Fincham, V. J., Carragher, N. O., & Wyke, J. A. (2002) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm779 · Cited by 305
Lee, H., Shi, W., Tontonoz, P., Wang, S., Subbanagounder, G., Hedrick, C. C., Hama, S. Y., Borromeo, C. M., Evans, R. M., Berliner, J. A., et al. (2000) · Circulation Research · doi:10.1161/01.res.87.6.516 · Cited by 292
Self-citationDebets, R., Timans, J. C., Homey, B., Zurawski, S., Sana, T. R., Lo, S., Wagner, J., Edwards, G., Clifford, T., Menon, S., et al. (2001) · The Journal of Immunology · doi:10.4049/jimmunol.167.3.1440 · Cited by 283
JAKs, STATs and Src kinases in hematopoiesis
Rane, S. G., & Reddy, E. P. (2002) · Oncogene · doi:10.1038/sj.onc.1205398 · Cited by 245
In Vivo Evidence for a Role of Toll-Like Receptor 4 in the Development of Intimal Lesions
Vink, A., Schoneveld, A. H., van der Meer, J. J., van Middelaar, B. J., Sluijter, J. P. G., Smeets, M. B., Quax, P. H. A., Lim, S. K., Borst, C., Pasterkamp, G., et al. (2002) · Circulation · doi:10.1161/01.cir.0000032146.75113.ee · Cited by 243
Hofer-Warbinek, R., Schmid, J. A., Stehlik, C., Binder, B. R., Lipp, J., & de Martin, R. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.m910346199 · Cited by 231
Bochkov, V. N., Mechtcheriakova, D., Lucerna, M., Huber, J., Malli, R., Graier, W. F., Hofer, E., Binder, B. R., & Leitinger, N. (2002) · Blood · doi:10.1182/blood.v99.1.199 · Cited by 202
Subbanagounder, G., Wong, J. W. H., Lee, H. C., Faull, K. F., Miller, E. R., Witztum, J. L., & Berliner, J. A. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m107602200 · Cited by 183
Self-citationSpecific Phospholipid Oxidation Products Inhibit Ligand Activation of Toll-Like Receptors 4 and 2
Walton, K. A., Cole, A. L., Yeh, M. W., Subbanagounder, G., Krutzik, S. R., Modlin, R. L., Lucas, R. M., Nakai, J., Smart, E. J., Vora, D. K., et al. (2003) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000079340.80744.b8 · Cited by 183
Self-citationEvidence for a Role of Phospholipid Oxidation Products in Atherogenesis
Berliner, J. A. (2001) · Trends in Cardiovascular Medicine · doi:10.1016/s1050-1738(01)00098-6 · Cited by 182
Self-citationWalton, K. A., Hsieh, X., Gharavi, N. M., Wang, S. V., Wang, G., Yeh, M. W., Cole, A. L., & Berliner, J. A. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.m300738200 · Cited by 169
Self-citationShih, P. T., Elices, M. J., Fang, Z. T., Ugarova, T. P., Strahl, D., Territo, M., Frank, J. S., Kovach, N. L., Cabañas, C., Berliner, J. A., et al. (1999) · Journal of Clinical Investigation · doi:10.1172/jci5710 · Cited by 148
Self-citationMullings, R. E., Wilson, S. J., Puddicombe, S. M., Lordan, J. L., Bucchieri, F., Djukanović, R., Howarth, P. H., Harper, S. J., Holgate, S. T., & Davies, D. E. (2001) · Journal of Allergy and Clinical Immunology · doi:10.1067/mai.2001.119554 · Cited by 142
The many faces of Src: multiple functions of a prototypical tyrosine kinase
Schwartzberg, P. L. (1998) · Oncogene · doi:10.1038/sj.onc.1202176 · Cited by 141
CD19 regulates innate immunity by the toll-like receptor RP105 signaling in B lymphocytes
Yazawa, N. (2003) · Blood · doi:10.1182/blood-2002-11-3573 · Cited by 122
Cole, A. L., Subbanagounder, G., Mukhopadhyay, S., Berliner, J. A., & Vora, D. K. (2003) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/01.atv.0000081215.45714.71 · Cited by 121
Self-citationYeh, M. W., Leitinger, N., de Martin, R., Onai, N., Matsushima, K., Vora, D. K., Berliner, J. A., & Reddy, S. T. (2001) · Arteriosclerosis Thrombosis and Vascular Biology · doi:10.1161/hq1001.097027 · Cited by 120
Self-citationWang, Y.-Z., Wharton, W., Garcia, R., Kraker, A. J., Jove, R., & Pledger, W. J. (2000) · Oncogene · doi:10.1038/sj.onc.1203548 · Cited by 113
Masamune, A., Kikuta, K., Satoh, M., Satoh, A., & Shimosegawa, T. (2002) · Journal of Pharmacology and Experimental Therapeutics · doi:10.1124/jpet.302.1.36 · Cited by 110
Bioactive products of phospholipid oxidation: isolation, identification, measurement and activities
Subbanagounder, G., Watson, A. D., & Berliner, J. A. (2000) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(00)00233-1 · Cited by 99
Self-citationOkutani, Y., Kitanaka, A., Tanaka, T., Kamano, H., Ohnishi, H., Kubota, Y., Ishida, T., & Takahara, J. (2001) · Oncogene · doi:10.1038/sj.onc.1204807 · Cited by 95
Mazière, C., Alimardani, G., Dantin, F., Dubois, F., Conte, M.-A., & Mazière, J. (1999) · FEBS Letters · doi:10.1016/s0014-5793(99)00324-5 · Cited by 73
c-Src Activates both STAT1 and STAT3 in PDGF-Stimulated NIH3T3 Cells
Cirri, P., Chiarugi, P., Marra, F., Raugei, G., Camici, G., Manao, G., & Ramponi, G. (1997) · Biochemical and Biophysical Research Communications · doi:10.1006/bbrc.1997.7493 · Cited by 67
Function and Regulation of Prostaglandin Synthase-2
Herschman, H. R., Reddy, S. T., & Xie, W. (1997) · Advances in experimental medicine and biology · doi:10.1007/978-1-4899-1813-0_9 · Cited by 63
Self-citationLiu, R., Aupperle, K. R., & Terkeltaub, R. A. (2001) · Journal of Leukocyte Biology · doi:10.1189/jlb.70.6.961 · Cited by 58
Hazeki, K., Masuda, N., Funami, K., Sukenobu, N., Matsumoto, M., Akira, S., Takeda, K., Seya, T., & Hazeki, O. (2003) · European Journal of Immunology · doi:10.1002/eji.200323375 · Cited by 56
TRAF6 and C‐SRC induce synergistic AP‐1 activation via PI3‐kinase–AKT–JNK pathway
Funakoshi‐Tago, M., Tago, K., Sonoda, Y., Tominaga, S., & Kasahara, T. (2003) · European Journal of Biochemistry · doi:10.1046/j.1432-1033.2003.03487.x · Cited by 49
Vaingankar, S. M., & Martins‐Green, M. M. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.9.5226 · Cited by 41
Velichko, S., Wagner, T. C., Turkson, J., Jove, R., & Croze, E. (2002) · Journal of Biological Chemistry · doi:10.1074/jbc.m204578200 · Cited by 36
Blaber, R., Stylianou, E., Clayton, A., & Steadman, R. (2003) · Journal of the American Society of Nephrology · doi:10.1097/01.asn.0000040595.35207.62 · Cited by 36
Expression of v-src in T cells correlates with nuclear expression of NF-kappa B.
Eicher, D. M., Tan, T., Rice, N. R., O’Shea, J. J., & Kennedy, I. C. S. (1994) · The Journal of Immunology · doi:10.4049/jimmunol.152.6.2710 · Cited by 32
Schmid, P., Cox, D., McMaster, G. K., & Itin, P. H. (1993) · Archives of Dermatological Research · doi:10.1007/bf00371833 · Cited by 31
Expression of ETS-1 Related to Angiogenesis in Uterine Endometrium during the Menstrual Cycle
Fujimoto, J., Aoki, I., Toyoki, H., Khatun, S., Sato, E., & Tamaya, T. (2003) · Journal of Biomedical Science · doi:10.1007/bf02256451 · Cited by 23
Myers, S. J., & Stanley, K. K. (1999) · Atherosclerosis · doi:10.1016/s0021-9150(98)00331-1 · Cited by 21
Cited by
Sorted by citations
Cytokines in Atherosclerosis: Pathogenic and Regulatory Pathways
Tedgui, A., & Mallat, Z. (2006) · Physiological Reviews · doi:10.1152/physrev.00024.2005 · Cited by 1,751
Aggarwal, B. B., Vijayalekshmi, R. V., & Sung, B. (2009) · Clinical Cancer Research · doi:10.1158/1078-0432.ccr-08-0149 · Cited by 812
Src kinases as therapeutic targets for cancer
Kim, L. C., Song, L., & Haura, E. B. (2009) · Nature Reviews Clinical Oncology · doi:10.1038/nrclinonc.2009.129 · Cited by 700
Generation and Biological Activities of Oxidized Phospholipids
Bochkov, V. N., Oskolkova, O., Birukov, K. G., Levonen, A., Binder, C. J., & Stöckl, J. (2009) · Antioxidants and Redox Signaling · doi:10.1089/ars.2009.2597 · Cited by 625
Andoh, A., Zhang, Z., Inatomi, O., Fujino, S., Deguchi, Y., Araki, Y., Tsujikawa, T., Kitoh, K., Kim‐Mitsuyama, S., Takayanagi, A., et al. (2005) · Gastroenterology · doi:10.1053/j.gastro.2005.06.071 · Cited by 480
Metadata
- Authors
- Yeh, M., Gharavi, N. M., Choi, J., Hsieh, X., Reed, E., Mouillesseaux, K. P., Cole, A. L., Reddy, S. T., & Berliner, J. A.
- Affiliations
- University of California, Los Angeles (US)
- Corresponding author
- Berliner, Judith A.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2004-07
- Volume
- 279
- Issue
- 29
- Pages
- 30175-30181
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2013661403
- Cited by
- 116
- References
- 46
- Citations by year
- 2025: 1 · 2023: 3 · 2022: 1 · 2021: 1 · 2020: 1 · 2018: 1 · 2017: 3 · 2016: 2 · 2015: 6 · 2014: 8 · 2013: 4 · 2012: 10
- FWCI
- 4.57
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Immunology › Atherosclerosis and Cardiovascular Diseases
- Life Sciences › Immunology and Microbiology › Immunology › Cytokine Signaling Pathways and Interactions
- Health Sciences › Medicine › Physiology (medical) › Nitric Oxide and Endothelin Effects
- Keywords
- oxidized phospholipids, interleukin-8, Src kinase, STAT3, endothelial cells, Src family kinases, NF-κB-independent signaling, STAT3 nuclear translocation, v-Src, inflammation, vasoactive mediators
- MeSH terms
- CSK Tyrosine-Protein Kinase, Cyclic AMP, Cell Nucleus, Cells, Cultured, Cytoplasm, DNA-Binding Proteins, Endothelium, Vascular, Enzyme-Linked Immunosorbent Assay, Genes, Dominant, Humans, Inflammation, Kinetics, Models, Biological, Oxygen, Phosphatidylcholines, Phospholipids, Phosphorylation, Plasmids, Protein-Tyrosine Kinases, Time Factors, Transfection, Tyrosine, Blotting, Western, Signal Transduction, Trans-Activators, Interleukin-8, NF-kappa B, Protein Structure, Tertiary, src-Family Kinases, STAT3 Transcription Factor
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
