The DOI matches its Crossref record; 26 of the 26 references it cites were found in a registry or an index.
Identification and Characterization of a Novel Monoamine Transporter in the Human Brain
Open accessAbstract
Precise control of monoamine neurotransmitter levels in the extracellular fluids of the brain is critical in maintaining efficient and robust neurotransmission. High affinity transporters in the solute carrier SLC6A family function in removing monoamines from the neurosynaptic cleft. Emerging evidence suggests that these transporters are only one part of a system of transporters that work in concert to maintain brain homeostasis of monoamines. Here we report the cloning and characterization of a new human plasma membrane monoamine transporter, PMAT. The PMAT cDNA encodes a protein of 530 amino acid residues with 10-12 transmembrane segments. PMAT is not homologous to known neurotransmitter transporters but exhibits low homology to members of the equilibrative nucleoside transporter family. When expressed in Madin-Darby canine kidney cells and Xenopus laevis oocytes, PMAT efficiently transports serotonin (K(m) = 114 mum), dopamine (K(m) = 329 mum), and the neurotoxin 1-methyl-4-phenylpyridinium (K(m) = 33 mum). In contrast, there is no significant interaction of PMAT with nucleosides or nucleobases. PMAT-mediated monoamine transport does not require Na(+) or Cl(-) but appears to be sensitive to changes in membrane potential. Northern blot analysis showed that PMAT is predominantly expressed in the human brain and widely distributed in the central nervous system. These studies demonstrate that PMAT may be a novel low affinity transporter for biogenic amines, which, under certain conditions, might supplement the role of the high affinity transporters in the brain.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences
Mammalian Gene Collection (MGC) Program Team* (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.242603899 · Cited by 1,749
Neurotransmitter Transporters: Recent Progress
Amara, S. G., & Kuhar, M. J. (1993) · Annual Review of Neuroscience · doi:10.1146/annurev.ne.16.030193.000445 · Cited by 1,082
Plasma membrane monoamine transporters: structure, regulation and function
Torres, G. E., Gainetdinov, R. R., & Caron, M. G. (2003) · Nature Reviews Neuroscience · doi:10.1038/nrn1008 · Cited by 939
The Neurobiology of Slow Synaptic Transmission
Greengard, P. (2001) · Science · doi:10.1126/science.294.5544.1024 · Cited by 867
The equilibrative nucleoside transporter family, SLC29
Beal, P. R., Yao, S. Y. M., Baldwin, S. A., Young, J. D., King, A. E., & Cass, C. E. (2004) · Pfl�gers Archiv European Journal of Physiology · doi:10.1007/s00424-003-1103-2 · Cited by 710
Profound neuronal plasticity in response to inactivation of the dopamine transporter
Jones, S. R., Gainetdinov, R. R., Jaber, M., Giros, B., Wightman, R. M., & Caron, M. G. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.7.4029 · Cited by 600
The SLC22 drug transporter family
Koepsell, H., & Endou, H. (2004) · Pfl�gers Archiv European Journal of Physiology · doi:10.1007/s00424-003-1089-9 · Cited by 525
Sora, I., Hall, F. S., Andrews, A. M., Itokawa, M., Li, X.-F., Wei, H.-B., Wichems, C., Lesch, K.-P., Murphy, D. L., & Uhl, G. R. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.091039298 · Cited by 477
Griffiths, M., Beaumont, N., Yao, S. Y. M., Sundaram, M., Boumah, C. E., Davies, A., Kwong, F. Y. P., Coe, I., Cass, C. E., Young, J. D., et al. (1997) · Nature Medicine · doi:10.1038/nm0197-89 · Cited by 422
Molecular Physiology of Norepinephrine and Serotonin Transporters
Blakely, R. D., De Felice, L. J., & Hartzell, H. C. (1994) · Journal of Experimental Biology · doi:10.1242/jeb.196.1.263 · Cited by 404
Cited by
Metformin and the gastrointestinal tract
McCreight, L. J., Bailey, C. J., & Pearson, E. R. (2016) · Diabetologia · doi:10.1007/s00125-015-3844-9 · Cited by 733
Nishimura, M., & Naito, S. (2005) · Drug Metabolism and Pharmacokinetics · doi:10.2133/dmpk.20.452 · Cited by 388
Embracing diversity in the 5-HT neuronal system
Okaty, B. W., Commons, K. G., & Dymecki, S. M. (2019) · Nature Reviews Neuroscience · doi:10.1038/s41583-019-0151-3 · Cited by 319
The neuronal genome of Caenorhabditis elegans
Hobert, O. (2013) · WormBook · doi:10.1895/wormbook.1.161.1 · Cited by 279
Catecholamine/Serotonin Interactions
Hensler, J. G., Artigas, F., Bortolozzi, A., Daws, L. C., De Deurwaerdère, P., Milan, L., Navailles, S., & Koek, W. (2013) · Advances in Pharmacology · doi:10.1016/b978-0-12-411512-5.00009-9 · Cited by 73
Metadata
- Authors
- Engel, K., Zhou, M., & Wang, J.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2004-11
- Volume
- 279
- Issue
- 48
- Pages
- 50042-50049
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W1966752296
- Cited by
- 267
- References
- 26
- FWCI
- 4.23
- Type
- journal article
- Language
- en
- Open access
- hybrid
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Identification and Characterization of a Novel Monoamine Transporter in the Human Brain. https://app.cochat.ai/papers/10.1074/jbc.m407913200
- The paper
- Engel, K., Zhou, M., & Wang, J. (2004). Identification and Characterization of a Novel Monoamine Transporter in the Human Brain. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m407913200
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
