The DOI matches its Crossref record; 59 of the 59 references it cites were found in a registry or an index.
Resveratrol and Estradiol Rapidly Activate MAPK Signaling through Estrogen Receptors α and β in Endothelial Cells
Open accessAbstract
Vascular endothelial cells (EC) are an important target of estrogen action through both the classical genomic (i.e. nuclear-initiated) activities of estrogen receptors alpha and beta (ERalpha and ERbeta) and the rapid "non-genomic" (i.e. membrane-initiated) activation of ER that stimulates intracellular phosphorylation pathways. We tested the hypothesis that the red wine polyphenol trans-resveratrol activates MAPK signaling via rapid ER activation in bovine aortic EC, human umbilical vein EC, and human microvascular EC. We report that bovine aortic EC, human umbilical vein EC, and human microvascular EC express ERalpha and ERbeta. We demonstrate that resveratrol and estradiol (E(2)) rapidly activated MAPK in a MEK-1, Src, matrix metalloproteinase, and epidermal growth factor receptor-dependent manner. Importantly, resveratrol activated MAPK and endothelial nitric-oxide synthase (eNOS) at nm concentrations (i.e. an order of magnitude less than that required for ER genomic activity) and concentrations possibly achieved transiently in serum following oral red wine consumption. Co-treatment with ER antagonists ICI 182,780 or 4-hydroxytamoxifen blocked resveratrol- or E(2)-induced MAPK and eNOS activation, indicating ER dependence. We demonstrate for the first time that ERalpha-and ERbeta-selective agonists propylpyrazole triol and diarylpropionitrile, respectively, stimulate MAPK and eNOS activity. A red but not a white wine extract also activated MAPK, and activity was directly correlated with the resveratrol concentration. These data suggest that ER may play a role in the rapid effects of resveratrol in EC and that some of the atheroprotective effects of resveratrol may be mediated through rapid activation of ER signaling in EC.
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Kuiper, G. G. J. M., Carlsson, B., Grandien, K., Enmark, E., Häggblad, J., Nilsson, S., & Gustafsson, J.-A. (1997) · Endocrinology · doi:10.1210/endo.138.3.4979 · Cited by 4,442
Gehm, B. D., McAndrews, J. M., Chien, P.-Y., & Jameson, J. L. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.25.14138 · Cited by 1,063
Chen, Z., Yuhanna, I. S., Galcheva-Gargova, Z., Karas, R. H., Mendelsohn, M. E., & Shaul, P. W. (1999) · Journal of Clinical Investigation · doi:10.1172/jci5347 · Cited by 923
Absorption of three wine-related polyphenols in three different matrices by healthy subjects
Goldberg, D. M., Yan, J., & Soleas, G. J. (2003) · Clinical Biochemistry · doi:10.1016/s0009-9120(02)00397-1 · Cited by 683
Meyers, M. J., Sun, J., Carlson, K. E., Marriner, G. A., Katzenellenbogen, B. S., & Katzenellenbogen, J. A. (2001) · Journal of Medicinal Chemistry · doi:10.1021/jm010254a · Cited by 678
Wallerath, T., Deckert, G., Ternes, T., Anderson, H., Li, H., Witte, K., & Förstermann, U. (2002) · Circulation · doi:10.1161/01.cir.0000029925.18593.5c · Cited by 666
Haynes, M. P., Sinha, D., Russell, K. S., Collinge, M., Fulton, D., Morales-Ruiz, M., Sessa, W. C., & Bender, J. R. (2000) · Circulation Research · doi:10.1161/01.res.87.8.677 · Cited by 575
Estrogen Modulation of Endothelial Nitric Oxide Synthase
Chambliss, K. L., & Shaul, P. W. (2002) · Endocrine Reviews · doi:10.1210/er.2001-0045 · Cited by 569
Resveratrol Acts as a Mixed Agonist/Antagonist for Estrogen Receptors α and β*
Bowers, J. L., Tyulmenkov, V. V., Jernigan, S. C., & Klinge, C. M. (2000) · Endocrinology · doi:10.1210/endo.141.10.7721 · Cited by 526
Self-citationBiological Effects of Resveratrol
Bhat, K. P. L., Kosmeder, J. W., & Pezzuto, J. M. (2001) · Antioxidants & Redox Signaling · doi:10.1089/152308601317203567 · Cited by 507
Cited by
Biological activity of piceatannol: Leaving the shadow of resveratrol
Piotrowska, H., Kucinska, M., & Murias, M. (2012) · Mutation Research/Reviews in Mutation Research · doi:10.1016/j.mrrev.2011.11.001 · Cited by 476
Flavonoids: modulators of brain function?
Spencer, J. P. E. (2008) · British Journal of Nutrition · doi:10.1017/s0007114508965776 · Cited by 384
Thomas, S. R., Witting, P. K., & Drummond, G. R. (2008) · Antioxidants & Redox Signaling · doi:10.1089/ars.2008.2027 · Cited by 383
Resveratrol and Its Effects on the Vascular System
Breuss, J. M., Atanasov, A. G., & Uhrin, P. (2019) · International Journal of Molecular Sciences · doi:10.3390/ijms20071523 · Cited by 328
Estrogenic endocrine disruptors: Molecular mechanisms of action
Kiyama, R., & Wada-Kiyama, Y. (2015) · Environment International · doi:10.1016/j.envint.2015.05.012 · Cited by 287
Metadata
- Authors
- Klinge, C. M., Blankenship, K. A., Risinger, K. E., Bhatnagar, S., Noisin, E. L., Sumanasekera, W. K., Zhao, L., Brey, D. M., & Keynton, R. S.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2005-03
- Volume
- 280
- Issue
- 9
- Pages
- 7460-7468
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2089633892
- Cited by
- 296
- References
- 59
- FWCI
- 8.92
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: Resveratrol and Estradiol Rapidly Activate MAPK Signaling through Estrogen Receptors α and β in Endothelial Cells. https://app.cochat.ai/papers/10.1074/jbc.m411565200
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- Klinge, C. M., Blankenship, K. A., Risinger, K. E., Bhatnagar, S., Noisin, E. L., Sumanasekera, W. K., Zhao, L., Brey, D. M., & Keynton, R. S. (2005). Resveratrol and Estradiol Rapidly Activate MAPK Signaling through Estrogen Receptors α and β in Endothelial Cells. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m411565200
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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