The DOI matches its Crossref record; 71 of the 71 references it cites were found in a registry or an index.
Extracellular Matrix Remodeling by Human Granzyme B via Cleavage of Vitronectin, Fibronectin, and Laminin
Open accessAbstract
Human granzyme B (GrB) released from cytotoxic lymphocytes plays a key role in the induction of target cell apoptosis when internalized in the presence of perforin. Here we demonstrate that GrB also possesses a potent extracellular matrix remodeling activity. Both native and recombinant GrB caused detachment of immortalized and transformed cell lines, primary endothelial cells, and chondrocytes. Cell detachment by GrB induced endothelial cell death (anoikis). GrB also inhibited tumor cell spreading, migration, and invasion in vitro. Investigation into the underlying mechanism revealed that GrB efficiently cleaves three proteins involved in extracellular matrix structure and function: vitronectin, fibronectin, and laminin. In vitronectin, GrB cleaves after an Arg-Lys-Asp (RGD) motif, which is part of the integrin-binding site found in matrix proteins. We propose that targeting of the integrin-extracellular matrix interface by GrB may allow perforin-independent killing of target cells via anoikis, restrict motility of tumor cells, facilitate lymphocyte migration, or directly reduce virus infectivity. It may also contribute to tissue destruction in diseases in which extracellular GrB is evident, such as rheumatoid arthritis and atherosclerosis.
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Cites
Integrins αvβ3 and αvβ5 promote adenovirus internalization but not virus attachment
Wickham, T. J., Mathias, P., Cheresh, D. A., & Nemerow, G. R. (1993) · Cell · doi:10.1016/0092-8674(93)90231-e · Cited by 2,199
Pankov, R., & Yamada, K. M. (2002) · Journal of Cell Science · doi:10.1242/jcs.00059 · Cited by 1,953
Role of integrins in cell invasion and migration
Hood, J. D., & Cheresh, D. A. (2002) · Nature Reviews Cancer · doi:10.1038/nrc727 · Cited by 1,777
The extracellular matrix as a cell survival factor.
Meredith, J. E., Fazeli, B., & Schwartz, M. A. (1993) · Molecular Biology of the Cell · doi:10.1091/mbc.4.9.953 · Cited by 1,526
Plow, E. F., Haas, T. A., Zhang, L., Loftus, J., & Smith, J. W. (2000) · Journal of Biological Chemistry · doi:10.1074/jbc.r000003200 · Cited by 1,356
Lymphocyte-Mediated Cytotoxicity
Russell, J. H., & Ley, T. J. (2002) · Annual Review of Immunology · doi:10.1146/annurev.immunol.20.100201.131730 · Cited by 1,122
Function and interactions of integrins
van der Flier, A., & Sonnenberg, A. (2001) · Cell and Tissue Research · doi:10.1007/s004410100417 · Cited by 1,039
Structure and biological activity of basement membrane proteins
TIMPL, R. (1989) · European Journal of Biochemistry · doi:10.1111/j.1432-1033.1989.tb14673.x · Cited by 973
The ABCs of granule-mediated cytotoxicity: new weapons in the arsenal
Lieberman, J. (2003) · Nature Reviews Immunology · doi:10.1038/nri1083 · Cited by 789
Miner, J. H., Patton, B. L., Lentz, S. I., Gilbert, D. J., Snider, W. D., Jenkins, N. A., Copeland, N. G., & Sanes, J. R. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.137.3.685 · Cited by 660
Cited by
Granzymes in cancer and immunity
Cullen, S. P., Brunet, M., & Martin, S. J. (2010) · Cell Death & Differentiation · doi:10.1038/cdd.2009.206 · Cited by 391
Sick fat: the good and the bad of old and new circulating markers of adipose tissue inflammation
Barchetta, I., Cimini, F. A., Ciccarelli, G., Baroni, M. G., & Cavallo, M. G. (2019) · Journal of Endocrinological Investigation · doi:10.1007/s40618-019-01052-3 · Cited by 77
Ang, L. S., Boivin, W. A., Williams, S. J., Zhao, H., Abraham, T., Carmine-Simmen, K., McManus, B. M., Bleackley, R. C., & Granville, D. J. (2011) · Cell Death & Disease · doi:10.1038/cddis.2011.88 · Cited by 55
Metadata
- Authors
- Buzza, M. S., Zamurs, L., Sun, J., Bird, C. H., Smith, A. I., Trapani, J. A., Froelich, C. J., Nice, E. C., & Bird, P. I.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2005-06
- Volume
- 280
- Issue
- 25
- Pages
- 23549-23558
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2165790227
- Cited by
- 266
- References
- 71
- FWCI
- 3.52
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- CoChat. (2026). Citation check: Extracellular Matrix Remodeling by Human Granzyme B via Cleavage of Vitronectin, Fibronectin, and Laminin. https://app.cochat.ai/papers/10.1074/jbc.m412001200
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- Buzza, M. S., Zamurs, L., Sun, J., Bird, C. H., Smith, A. I., Trapani, J. A., Froelich, C. J., Nice, E. C., & Bird, P. I. (2005). Extracellular Matrix Remodeling by Human Granzyme B via Cleavage of Vitronectin, Fibronectin, and Laminin. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m412001200
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
