The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Conformational Dependence of Intracellular NADH on Metabolic State Revealed by Associated Fluorescence Anisotropy*♦
Open accessAbstract
Global analysis of fluorescence and associated anisotropy decays of intrinsic tissue fluorescence offers a sensitive and non-invasive probe of the metabolically critical free/enzyme-bound states of intracellular NADH in neural tissue. Using this technique, we demonstrate that the response of NADH to the metabolic transition from normoxia to hypoxia is more complex than a simple increase in NADH concentration. The concentration of free NADH, and that of an enzyme bound form with a relatively low lifetime, increases preferentially over that of other enzyme bound NADH species. Concomitantly, the intracellular viscosity is reduced, likely due to the osmotic swelling of mitochondria. These conformation and environmental changes effectively decrease the tissue fluorescence average lifetime, causing the usual total fluorescence increase measurements to significantly underestimate the calculated concentration increase. This new discrimination of changes in NADH concentration, conformation, and environment provides the foundation for quantitative functional imaging of neural energy metabolism.
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Principles of Fluorescence Spectroscopy
Lakowicz, J. R. (1999) · Springer eBooks · doi:10.1007/978-1-4757-3061-6 · Cited by 26,947
Relationship between Absorption Intensity and Fluorescence Lifetime of Molecules
Strickler, S. J., & Berg, R. A. (1962) · The Journal of Chemical Physics · doi:10.1063/1.1733166 · Cited by 2,481
Williamson, D., Lund, P., & Krebs, H. (1967) · Biochemical Journal · doi:10.1042/bj1030514 · Cited by 1,871
Chance, B., Cohen, P., Jobsis, F., & Schoener, B. (1962) · Science · doi:10.1126/science.137.3529.499 · Cited by 946
Respiratory Enzymes in Oxidative Phosphorylation
Chance, B., & Baltscheffsky, H. (1958) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(18)64738-6 · Cited by 887
Neural activity triggers neuronal oxidative metabolism followed by astrocytic glycolysis
Kasischke, K. A., Vishwasrao, H. D., Fisher, P. J., Zipfel, W. R., & Webb, W. W. (2004) · Science · doi:10.1126/science.1096485 · Cited by 742
Self-citationRegulation of Corepressor Function by Nuclear NADH
Zhang, Q., Piston, D. W., & Goodman, R. H. (2002) · Science · doi:10.1126/science.1069300 · Cited by 620
Simultaneous analysis of multiple fluorescence decay curves: A global approach
Knutson, J. R., Beechem, J. M., & Brand, L. (1983) · Chemical Physics Letters · doi:10.1016/0009-2614(83)87454-5 · Cited by 571
Determinants of the translational mobility of a small solute in cell cytoplasm.
Kao, H. P., Abney, J. R., & Verkman, A. S. (1993) · The Journal of cell biology · doi:10.1083/jcb.120.1.175 · Cited by 296
Rapid Diffusion of Green Fluorescent Protein in the Mitochondrial Matrix
Partikian, A., Ölveczky, B., Swaminathan, R., Li, Y., & Verkman, A. S. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.140.4.821 · Cited by 227
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Gordon, G. R. J., Choi, H. B., Rungta, R. L., Ellis-Davies, G. C. R., & MacVicar, B. A. (2008) · Nature · doi:10.1038/nature07525 · Cited by 744
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Heikal, A. A. (2010) · Biomarkers in Medicine · doi:10.2217/bmm.10.1 · Cited by 458
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Pöhlker, C., Huffman, J. A., & Pöschl, U. (2012) · Atmospheric Measurement Techniques · doi:10.5194/amt-5-37-2012 · Cited by 394
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Grivennikova, V. G., & Vinogradov, A. D. (2006) · Biochimica et Biophysica Acta (BBA) - Bioenergetics · doi:10.1016/j.bbabio.2006.03.013 · Cited by 379
Metadata
- Authors
- Vishwasrao, H. D., Heikal, A. A., Kasischke, K. A., & Webb, W. W.
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2005-07
- Volume
- 280
- Issue
- 26
- Pages
- 25119-25126
- ISSN
- 0021-9258 (ISSN-L)
- OpenAlex
- W2072376785
- Cited by
- 296
- References
- 32
- FWCI
- 9.76
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Vishwasrao, H. D., Heikal, A. A., Kasischke, K. A., & Webb, W. W. (2005). Conformational Dependence of Intracellular NADH on Metabolic State Revealed by Associated Fluorescence Anisotropy*♦. Journal of Biological Chemistry. https://doi.org/10.1074/jbc.m502475200
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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