Cited by 42Epigenetics and DNA MethylationGenomics and Chromatin DynamicsCancer-related gene regulation

Activation of a Methylated Promoter Mediated by a Sequence-specific DNA-binding Protein, RFX

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Niesen, Melissa I., Osborne, Aaron R., Yang, Hua, Rastogi, Shipra, Chellappan, Srikumar, Cheng, Jin Q., Boss, Jeremy M., Blanck, George·2005·Journal of Biological Chemistry

Abstract

The roles of eukaryotic DNA methylation in the repression of mRNA transcription and in the formation of heterochromatin have been extensively elucidated over the past several years. However, the role of DNA methylation in transcriptional activation remains a mystery. In particular, it is not known whether the transcriptional activation of methylated DNA is promoter-specific, depends directly on sequence-specific DNA-binding proteins, or is facilitated by the methylation. Here we report that the sequence-specific DNA-binding protein, RFX, previously shown to mediate the transition from an inactive to an active chromatin structure, activates a methylated promoter. RFX is capable of mediating enhanceosome formation on a methylated promoter, thereby mediating a transition from a methylation-dependent repression of the promoter to a methylation-dependent activation of the promoter. These results indicate novel roles for DNA methylation and sequence-specific DNA-binding proteins in transcriptional activation.

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  1. De Larco, J. E., Wuertz, B. R., Yee, D., Rickert, B. L., and Furcht, L. T. (2003) Proc. Natl. Acad. Sci. U. S. A. 100, 13988 -13993
  2. Scholl, T., Mahanta, S. K., and Strominger, J. L. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 6330 -6334

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Authors
Niesen, M. I., Osborne, A. R., Yang, H., Rastogi, S., Chellappan, S., Cheng, J. Q., Boss, J. M., & Blanck, G.
Affiliations
  • University of South Florida (US)
  • Emory University (US)
Corresponding author
Blanck, George
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2005-11
Volume
280
Issue
47
Pages
38914-38922
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2129969493
Cited by
42
References
51
Citations by year
2024: 1 · 2023: 3 · 2021: 3 · 2020: 4 · 2019: 4 · 2018: 3 · 2017: 1 · 2016: 4 · 2015: 5 · 2014: 2 · 2013: 4
FWCI
0.62
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • University of South Florida
  • Moffitt Cancer Center
  • National Institutes of Health (5r01ca081497-04, R01 CA81497, AI34000)
  • National Cancer Institute (R01 CA81497, R01CA063136, R01CA089242)
  • National Institute of General Medical Sciences (R01GM047310)
  • National Institute of Allergy and Infectious Diseases (R01AI034000)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Epigenetics and DNA Methylation
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cancer-related gene regulation
Keywords
DNA methylation, transcriptional activation, RFX, sequence-specific DNA-binding proteins, enhanceosome, chromatin structure, transcriptional repression, heterochromatin, promoter activation
MeSH terms
Regulatory Factor X Transcription Factors, B-Lymphocytes, Base Sequence, Binding Sites, Cell Line, DNA, DNA-Binding Proteins, Gene Expression Regulation, HLA-DR Antigens, Humans, Models, Biological, Molecular Sequence Data, Promoter Regions, Genetic, Transcription Factors, Transfection, DNA Methylation, Octamer Transcription Factor-1, YY1 Transcription Factor, HLA-DR alpha-Chains

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