Recombination Mediator and Rad51 Targeting Activities of a Human BRCA2 Polypeptide
Open accessAbstract
BRCA2 likely exerts its tumor suppressor function by enhancing the efficiency of the homology-directed repair of injured chromosomes. To help define the DNA repair role of BRCA2, we expressed and purified a polypeptide, BRC3/4-DBD, that harbors its BRC3 and BRC4 repeats and DNA binding domain. BRC3/4-DBD interacted with hRad51 and bound DNA with a distinct preference for single-stranded (ss) DNA. Importantly we demonstrated by biochemical means and electron microscopy that BRC3/4-DBD nucleates hRad51 onto ssDNA and acts as a recombination mediator in enabling hRad51 to utilize replication protein A-coated ssDNA as recombination substrate. These functions of BRC3/4-DBD required both the BRC repeats and the BRCA2 DNA binding domain. The results thus clarify the role of BRCA2 in Rad51-dependent DNA recombination and repair, and the experimental strategies described herein should be valuable for systematically deciphering this BRCA2 function.
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When dsDNA is present, BRCA1-BARD1 cannot target RAD51 to ssDNA, whereas BRCA2-DSS1 can.
“unlike BRCA2-DSS137,38, BRCA1-BARD1 is devoid of the ability to target RAD51 to ssDNA when dsDNA is present”
From BRCA1–BARD1 promotes RAD51-mediated homologous DNA pairing (2017) · cites this paper as [37]
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Identification of the breast cancer susceptibility gene BRCA2
Wooster, R., Bignell, G. R., Lancaster, J., Swift, S., Seal, S., Mangion, J., Collins, N., Gregory, S. G., Gumbs, C. E., Micklem, G., et al. (1995) · Nature · doi:10.1038/378789a0 · Cited by 3,645 · see citing works
Embryonic lethality and radiation hypersensitivity mediated by Rad51 in mice lacking Brca2
Sharan, S. K., Morimatsu, M., Albrecht, U., Lim, D., Regel, E., Dinh, C. Q., Sands, A., Gregor Eichele, C., Hasty, P., & Bradley, A. (1997) · Nature · doi:10.1038/386804a0 · Cited by 1,071 · see citing works
BRCA2 Is Required for Homology-Directed Repair of Chromosomal Breaks
Moynahan, M. E., Pierce, A. J., & Jasin, M. (2001) · Molecular Cell · doi:10.1016/s1097-2765(01)00174-5 · Cited by 1,007 · see citing works
Role of RAD52 Epistasis Group Genes in Homologous Recombination and Double-Strand Break Repair
Symington, L. S. (2002) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.66.4.630-670.2002 · Cited by 965 · see citing works
Catalysis of ATP-Dependent Homologous DNA Pairing and Strand Exchange by Yeast RAD51 Protein
Sung, P. (1994) · Science · doi:10.1126/science.8066464 · Cited by 869 · see citing works
Self-citationRole of BRCA2 in Control of the RAD51 Recombination and DNA Repair Protein
Davies, A. A., Masson, J., McIlwraith, M. J., Stasiak, A. Z., Stasiak, A., Venkitaraman, A. R., & West, S. C. (2001) · Molecular Cell · doi:10.1016/s1097-2765(01)00175-7 · Cited by 720 · see citing works
BRCA2 Function in DNA Binding and Recombination from a BRCA2-DSS1-ssDNA Structure
Yang, H., Jeffrey, P. D., Miller, J. J., Kinnucan, E., Sun, Y., Thomä, N. H., Zheng, N., Chen, P., Lee, W., & Pavletich, N. P. (2002) · Science · doi:10.1126/science.297.5588.1837 · Cited by 705 · see citing works
Human Rad51 Protein Promotes ATP-Dependent Homologous Pairing and Strand Transfer Reactions In Vitro
Baumann, P., Benson, F. E., & West, S. C. (1996) · Cell · doi:10.1016/s0092-8674(00)81394-x · Cited by 689 · see citing works
Recombinant replication protein A: expression, complex formation, and functional characterization
Henricksen, L. A., Umbricht, C. B., & Wold, M. S. (1994) · Journal of Biological Chemistry · doi:10.1016/s0021-9258(19)78100-9 · Cited by 597 · see citing works
Wong, A. K. C., Pero, R. S., Ormonde, P. A., Tavtigian, S. V., & Bartel, P. L. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.51.31941 · Cited by 539 · see citing works
Sung, P., & Robberson, D. L. (1995) · Cell · doi:10.1016/0092-8674(95)90434-4 · Cited by 501 · see citing works
Self-citationCDK-dependent phosphorylation of BRCA2 as a regulatory mechanism for recombinational repair
Esashi, F., Christ, N., Gannon, J., Liu, Y., Hunt, T., Jasin, M., & West, S. C. (2005) · Nature · doi:10.1038/nature03404 · Cited by 473 · see citing works
Rad51 Recombinase and Recombination Mediators
Sung, P., Krejčí, L., Komen, S. V., & Sehorn, M. G. (2003) · Journal of Biological Chemistry · doi:10.1074/jbc.r300027200 · Cited by 446 · see citing works
Self-citationBRCA2 is required for ionizing radiation-induced assembly of Rad51 complex in vivo.
Yuan, S. F., Lee, S. Y., Chen, G., Song, M., Tomlinson, G. E., & Lee, E. Y.-H. P. (1999) · PubMed · Cited by 409 · see citing works
Chen, P., Chen, C., Chen, Y., Xiao, J., Sharp, Z. D., & Lee, W. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.9.5287 · Cited by 394 · see citing works
Homologous repair of DNA damage and tumorigenesis:the BRCA connection
Jasin, M. (2002) · Oncogene · doi:10.1038/sj.onc.1206176 · Cited by 356 · see citing works
The BRCA2 homologue Brh2 nucleates RAD51 filament formation at a dsDNA–ssDNA junction
Yang, H., Li, Q., Fan, J., Holloman, W. K., & Pavletich, N. P. (2005) · Nature · doi:10.1038/nature03234 · Cited by 335 · see citing works
Sugiyama, T., Zaitseva, E. M., & Kowalczykowski, S. C. (1997) · Journal of Biological Chemistry · doi:10.1074/jbc.272.12.7940 · Cited by 307 · see citing works
der Zwet, M. K.-V., Overkamp, W. J., van Lange, R. E. E., Essers, J., van Duijn-Goedhart, A., Wiggers, I., Swaminathan, S., van Buul, P. V., Errami, A., Tan, R., et al. (2002) · Molecular and Cellular Biology · doi:10.1128/mcb.22.2.669-679.2002 · Cited by 267 · see citing works
Xia, F., Taghian, D. G., DeFrank, J. S., Zeng, Z., Willers, H., Iliakis, G. E., & Powell, S. N. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.151253498 · Cited by 263 · see citing works
Activities of human recombination protein Rad51
Gupta, R. C., Bazemore, L. R., Golub, E. I., & Radding, C. M. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.2.463 · Cited by 260 · see citing works
Domain structure and dynamics in the helical filaments formed by RecA and Rad51 on DNA
Yu, X., Jacobs, S. A., West, S. C., Ogawa, T., & Egelman, E. H. (2001) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.111005398 · Cited by 254 · see citing works
RAB22 and RAB163/mouse BRCA2: Proteins that specifically interact with the RAD51 protein
Mizuta, R., LaSalle, J. M., Cheng, H.-L., Shinohara, A., Ogawa, H., Copeland, N. G., Jenkins, N. A., Lalande, M., & Alt, F. W. (1997) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.94.13.6927 · Cited by 235 · see citing works
Ribonuclease S‐peptide as a carrier in fusion proteins
Kim, J., & Raines, R. T. (1993) · Protein Science · doi:10.1002/pro.5560020307 · Cited by 199 · see citing works
Roles of ATP binding and ATP hydrolysis in human Rad51 recombinase function
Chi, P., Komen, S. V., Sehorn, M. G., Sigurðsson, S., & Sung, P. (2006) · DNA repair · doi:10.1016/j.dnarep.2005.11.005 · Cited by 187 · see citing works
Self-citationMarston, N. J., Richards, W. J., Hughes, D. A., Bertwistle, D., Marshall, C. J., & Ashworth, A. (1999) · Molecular and Cellular Biology · doi:10.1128/mcb.19.7.4633 · Cited by 186 · see citing works
Basis for Avid Homologous DNA Strand Exchange by Human Rad51 and RPA
Sigurðsson, S., Trujillo, K. M., Song, B., Stratton, S. A., & Sung, P. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.m010011200 · Cited by 171 · see citing works
Self-citationDSS1 is required for the stability of BRCA2
Li, J., Zou, C., Bai, Y., Wazer, D. E., Band, V., & Gao, Q. (2005) · Oncogene · doi:10.1038/sj.onc.1209153 · Cited by 136 · see citing works
Sequence fingerprints in BRCA2 and RAD51: implications for DNA repair and cancer
Lo, T., Pellegrini, L., Venkitaraman, A. R., & Blundell, T. L. (2003) · DNA repair · doi:10.1016/s1568-7864(03)00097-1 · Cited by 118 · see citing works
The Essential Functions of Human Rad51 Are Independent of ATP Hydrolysis
Morrison, C. G., Shinohara, A., Sonoda, E., Yamaguchi‐Iwai, Y., Takata, M., Weichselbaum, R. R., & Takeda, S. (1999) · Molecular and Cellular Biology · doi:10.1128/mcb.19.10.6891 · Cited by 116 · see citing works
The Fanconi road to cancer: Figure 1.Preprint
D’Andrea, A. D. (2003) · Genes & Development · doi:10.1101/gad.1128303 · Cited by 99 · see citing works
Differential and collaborative actions of Rad51 paralog proteins in cellular response to DNA damage
Yonetani, Y. (2005) · Nucleic Acids Research · doi:10.1093/nar/gki766 · Cited by 86 · see citing works
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Mechanism of Eukaryotic Homologous Recombination
Filippo, J. S., Sung, P., & Klein, H. L. (2008) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.77.061306.125255 · Cited by 1,686 · see citing works
Self-citationHomologous recombination in DNA repair and DNA damage tolerance
Li, X., & Heyer, W. (2008) · Cell Research · doi:10.1038/cr.2008.1 · Cited by 1,203 · see citing works
DNA Double-Strand Break Repair: All's Well that Ends Well
Wyman, C., & Kanaar, R. (2006) · Annual Review of Genetics · doi:10.1146/annurev.genet.40.110405.090451 · Cited by 806 · see citing works
Purified human BRCA2 stimulates RAD51-mediated recombination
Jensen, R. B., Carreira, A., & Kowalczykowski, S. C. (2010) · Nature · doi:10.1038/nature09399 · Cited by 715 · see citing works
Mechanism of homologous recombination: mediators and helicases take on regulatory functions
Sung, P., & Klein, H. L. (2006) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2008 · Cited by 697 · see citing works
Self-citationBRCA1–BARD1 promotes RAD51-mediated homologous DNA pairing
Zhao, W., Steinfeld, J. B., Liang, F., Chen, X., Maranon, D. G., Jian Ma, C., Kwon, Y., Rao, T., Wang, W., Sheng, C., et al. (2017) · Nature · doi:10.1038/nature24060 · Cited by 393 · see citing works
Self-citationHuman BRCA2 protein promotes RAD51 filament formation on RPA-covered single-stranded DNA
Liu, J., Doty, T., Gibson, B. A., & Heyer, W. (2010) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.1904 · Cited by 396 · see citing works
RAD52 Facilitates Mitotic DNA Synthesis Following Replication Stress
Bhowmick, R., Minocherhomji, S., & Hickson, I. D. (2016) · Molecular Cell · doi:10.1016/j.molcel.2016.10.037 · Cited by 379 · see citing works
Mechanisms of double-strand break repair in somatic mammalian cells
Hartlerode, A. J., & Scully, R. (2009) · Biochemical Journal · doi:10.1042/bj20090942 · Cited by 365 · see citing works
The antitumorigenic roles of BRCA1–BARD1 in DNA repair and replication
Tarsounas, M., & Sung, P. (2020) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-020-0218-z · Cited by 366 · see citing works
Self-citationThompson, L. H. (2012) · Mutation Research/Reviews in Mutation Research · doi:10.1016/j.mrrev.2012.06.002 · Cited by 357 · see citing works
Krawczyk, P. M., Eppink, B., Essers, J., Stap, J., Rodermond, H. M., Odijk, H., Zelensky, A. N., Chris van, B. R. E. E., Stalpers, L. J. A., Buist, M. R., et al. (2011) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1101053108 · Cited by 353 · see citing works
O'Donovan, P. J., & Livingston, D. M. (2010) · Carcinogenesis · doi:10.1093/carcin/bgq069 · Cited by 296 · see citing works
Cellular functions of the BRCA tumour-suppressor proteins
Boulton, S. J. (2006) · Biochemical Society Transactions · doi:10.1042/bst0340633 · Cited by 292 · see citing works
The BRC Repeats of BRCA2 Modulate the DNA-Binding Selectivity of RAD51
Carreira, A., Hilario, J., Amitani, I., Baskin, R. J., Shivji, M. K. K., Venkitaraman, A. R., & Kowalczykowski, S. C. (2009) · Cell · doi:10.1016/j.cell.2009.02.019 · Cited by 268 · see citing works
Unraveling the mechanism of BRCA2 in homologous recombination
Holloman, W. K. (2011) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.2096 · Cited by 220 · see citing works
Promotion of BRCA2-Dependent Homologous Recombination by DSS1 via RPA Targeting and DNA Mimicry
Zhao, W., Vaithiyalingam, S., San Filippo, J., Maranon, D. G., Jimenez-Sainz, J., Fontenay, G. V., Kwon, Y., Leung, S. G., Lu, L., Jensen, R. B., et al. (2015) · Molecular Cell · doi:10.1016/j.molcel.2015.05.032 · Cited by 177 · see citing works
Self-citationEnhancement of RAD51 recombinase activity by the tumor suppressor PALB2
Dray, E. V., Etchin, J., Wiese, C., Saro, D., Williams, G. J., Hammel, M., Yu, X., Galkin, V. E., Liu, D., Tsai, M.-S., et al. (2010) · Nature Structural & Molecular Biology · doi:10.1038/nsmb.1916 · Cited by 168 · see citing works
Self-citationThe BRCA Tumor Suppressor Network in Chromosome Damage Repair by Homologous Recombination
Zhao, W., Wiese, C., Kwon, Y., Hromas, R., & Sung, P. (2019) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-013118-111058 · Cited by 161 · see citing works
Self-citationWiese, C., Dray, E. V., Groesser, T., San Filippo, J., Shi, I. Y., Collins, D. W., Tsai, M.-S., Williams, G., Rydberg, B., Sung, P., et al. (2007) · OSTI OAI (U.S. Department of Energy Office of Scientific and Technical Information) · doi:10.1016/j.molcel.2007.08.027 · Cited by 149 · see citing works
Self-citationMolecular Genetics of Recombination
Heyer, W.-D. (2007) · Topics in current genetics · doi:10.1007/978-3-540-71021-9 · Cited by 126 · see citing works
RAD51C: a novel cancer susceptibility gene is linked to Fanconi anemia and breast cancer
Somyajit, K., Subramanya, S., & Nagaraju, G. (2010) · Carcinogenesis · doi:10.1093/carcin/bgq210 · Cited by 119 · see citing works
Hed1 regulates Rad51-mediated recombination via a novel mechanismPreprint
Busygina, V., Sehorn, M. G., Shi, I. Y., Tsubouchi, H., Roeder, G. S., & Sung, P. (2008) · Genes & Development · doi:10.1101/gad.1638708 · Cited by 111 · see citing works
Self-citationThe role of BRCA1 in DNA double-strand repair: Past and present
Caestecker, K. W., & Van de Walle, G. R. (2013) · Experimental Cell Research · doi:10.1016/j.yexcr.2012.11.013 · Cited by 108 · see citing works
Targeting Cancer with a Lupus Autoantibody
Hansen, J. E., Chan, G., Liu, Y., Hegan, D. C., Dalal, S., Dray, E. V., Kwon, Y., Xu, Y., Xu, X., Peterson-Roth, E., et al. (2012) · Science Translational Medicine · doi:10.1126/scitranslmed.3004385 · Cited by 107 · see citing works
Self-citationCell cycle-dependent control of homologous recombination
Zhao, X., Wei, C., Li, J., Xing, P., Li, J., Zheng, S., & Chen, X. (2017) · Acta Biochimica et Biophysica Sinica · doi:10.1093/abbs/gmx055 · Cited by 103 · see citing works
Minding the gap: The underground functions of BRCA1 and BRCA2 at stalled replication forks
Nagaraju, G., & Scully, R. (2007) · DNA repair · doi:10.1016/j.dnarep.2007.02.020 · Cited by 103 · see citing works
The Carboxyl Terminus of Brca2 Links the Disassembly of Rad51 Complexes to Mitotic Entry
Ayoub, N., Rajendra, E., Su, X., Jeyasekharan, A. D. D., Mahen, R., & Venkitaraman, A. R. (2009) · Current Biology · doi:10.1016/j.cub.2009.05.057 · Cited by 99 · see citing works
Wang, S., Durrant, W. E., Song, J., Spivey, N. W., & Dong, X. (2010) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1005978107 · Cited by 100 · see citing works
Rajendra, E., & Venkitaraman, A. R. (2009) · Nucleic Acids Research · doi:10.1093/nar/gkp873 · Cited by 90 · see citing works
Suppression of the DNA repair defects of BRCA2-deficient cells with heterologous protein fusions
Saeki, H., Siaud, N., Christ, N., Wiegant, W. W., van Buul, P. P. W., Han, M., Zdzienicka, M. Z., Stark, J. M., & Jasin, M. (2006) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0600298103 · Cited by 95 · see citing works
Cellular Redistribution of Rad51 in Response to DNA Damage
Gildemeister, O. S., Sage, J. M., & Knight, K. L. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.024646 · Cited by 94 · see citing works
Siaud, N., Barbera, M. A., Egashira, A., Lam, I., Christ, N., Schlacher, K., Xia, B., & Jasin, M. (2011) · PLoS Genetics · doi:10.1371/journal.pgen.1002409 · Cited by 86 · see citing works
Miné-Hattab, J., Disseau, L., Takahashi, M., Cappello, G., Dutreix, M., & Viovy, J. (2007) · Nucleic Acids Research · doi:10.1093/nar/gkm752 · Cited by 83 · see citing works
A region of human BRCA2 containing multiple BRC repeats promotes RAD51-mediated strand exchange
Shivji, M. K. K., Davies, O. R., Savill, J. M., Bates, D. L., Pellegrini, L., & Venkitaraman, A. R. (2006) · Nucleic Acids Research · doi:10.1093/nar/gkl505 · Cited by 83 · see citing works
Localization of recombination proteins and Srs2 reveals anti-recombinase function in vivo
Burgess, R. C., Lisby, M., Altmannová, V., Krejčí, L., Sung, P., & Rothstein, R. J. (2009) · The Journal of Cell Biology · doi:10.1083/jcb.200810055 · Cited by 81 · see citing works
Self-citationA novel role of the chromokinesin Kif4A in DNA damage response
Wu, G., Zhou, L., Khidr, L., Guo, X. E., Kim, W., Lee, Y. M., Krasieva, T. B., & Chen, P. (2008) · Cell Cycle · doi:10.4161/cc.7.13.6130 · Cited by 79 · see citing works
Gaines, W. A., Godin, S. K., Kabbinavar, F. F., Rao, T., VanDemark, A. P., Sung, P., & Bernstein, K. A. (2015) · Nature Communications · doi:10.1038/ncomms8834 · Cited by 75 · see citing works
Self-citationSingle-molecule imaging brings Rad51 nucleoprotein filaments into focus
Forget, A. L., & Kowalczykowski, S. C. (2010) · Trends in Cell Biology · doi:10.1016/j.tcb.2010.02.004 · Cited by 74 · see citing works
Samach, A., Melamed‐Bessudo, C., Avivi‐Ragolsky, N., Pietrokovski, S., & Levy, A. A. (2011) · The Plant Cell · doi:10.1105/tpc.111.091744 · Cited by 74 · see citing works
Shivji, M. K. K., Mukund, S. R., Rajendra, E., Chen, S., Short, J. M., Savill, J. M., Klenerman, D., & Venkitaraman, A. R. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0906208106 · Cited by 73 · see citing works
Inherited Mutations in Breast Cancer Genes—Risk and Response
Shuen, A. Y., & Foulkes, W. D. (2011) · Journal of Mammary Gland Biology and Neoplasia · doi:10.1007/s10911-011-9213-5 · Cited by 71 · see citing works
Tyrosine phosphorylation enhances RAD52‐mediated annealing by modulating its DNA binding
Honda, M., Okuno, Y., Yoo, J., Ha, T., & Spies, M. (2011) · The EMBO Journal · doi:10.1038/emboj.2011.238 · Cited by 68 · see citing works
Reconstitution of DNA Strand Exchange Mediated by Rhp51 Recombinase and Two Mediators
Kurokawa, Y., Murayama, Y., Haruta, N., Urabe, I., & Iwasaki, H. (2008) · PLoS Biology · doi:10.1371/journal.pbio.0060088 · Cited by 68 · see citing works
DNA repair pathways and hereditary cancer susceptibility syndromes
Spry, M. L. (2007) · Frontiers in bioscience · doi:10.2741/2380 · Cited by 64 · see citing works
Distinct binding of BRCA2 BRC repeats to RAD51 generates differential DNA damage sensitivity
Chatterjee, G., Jiménez-Sáinz, J., Presti, T., Nguyen, T. T. M., & Jensen, R. B. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkw242 · Cited by 63 · see citing works
Kwon, Y., Rösner, H. I., Zhao, W., Selemenakis, P., He, Z., Kawale, A. S., Katz, J. N., Rogers, C. M., Neal, F. E., Shabestari, A. B., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-36211-x · Cited by 65 · see citing works
Self-citationQuality control of DNA break metabolism: in the ‘end’, it's a good thing
Kanaar, R., Wyman, C., & Rothstein, R. J. (2008) · The EMBO Journal · doi:10.1038/emboj.2008.11 · Cited by 63 · see citing works
The Mei5-Sae3 Protein Complex Mediates Dmc1 Activity in Saccharomyces cerevisiae
Ferrari, S. R., Grubb, J., & Bishop, D. K. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.c900023200 · Cited by 61 · see citing works
Presynaptic filament dynamics in homologous recombination and DNA repair
Liu, J., Ehmsen, K. T., Heyer, W., & Morrical, S. W. (2011) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.3109/10409238.2011.576007 · Cited by 59 · see citing works
Molecular Anatomy of the Recombination Mediator Function of Saccharomyces cerevisiae Rad52
Seong, C., Sehorn, M. G., Plate, I., Shi, I. Y., Song, B., Chi, P., Mortensen, U. H., Sung, P., & Krejčí, L. (2008) · Journal of Biological Chemistry · doi:10.1074/jbc.m800763200 · Cited by 59 · see citing works
Self-citationLiu, Y., Gaines, W. A., Callender, T. L., Busygina, V., Oke, A., Sung, P., Fung, J. C., & Hollingsworth, N. M. (2014) · PLoS Genetics · doi:10.1371/journal.pgen.1004005 · Cited by 58 · see citing works
Self-citationRole for the Mammalian Swi5-Sfr1 Complex in DNA Strand Break Repair through Homologous Recombination
Akamatsu, Y., & Jasin, M. (2010) · PLoS Genetics · doi:10.1371/journal.pgen.1001160 · Cited by 58 · see citing works
A second DNA binding site in human BRCA2 promotes homologous recombination
Nicolai, C. V., Ehlén, Å., Martin, C. A., Zhang, X., & Carreira, A. (2016) · Nature Communications · doi:10.1038/ncomms12813 · Cited by 55 · see citing works
The role of BRCA2 in replication-coupled DNA interstrand cross-link repair in vitro
Čipák, L., Watanabe, N., & Bessho, T. (2006) · Nature Structural & Molecular Biology · doi:10.1038/nsmb1120 · Cited by 53 · see citing works
Guardians of the Genome: BRCA2 and Its Partners
Le, H. P., Heyer, W., & Liu, J. (2021) · Genes · doi:10.3390/genes12081229 · Cited by 52 · see citing works
Seong, C., Colavito, S. A., Kwon, Y., Sung, P., & Krejčí, L. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m109.032953 · Cited by 48 · see citing works
Self-citationAndreassen, P. R., Seo, J., Wiek, C., & Hanenberg, H. (2021) · Genes · doi:10.3390/genes12071034 · Cited by 49 · see citing works
A Boolean network model of the FA/BRCA pathway
Rodríguez, A., Sosa, D., Torres, L., Molina, B., Frı́as, S., & Mendoza, L. (2012) · Bioinformatics · doi:10.1093/bioinformatics/bts036 · Cited by 49 · see citing works
Špı́rek, M., Mlčoušková, J., Beláň, O., Gyimesi, M., Harami, G. M., Molnár, E., Nováček, J., Kovács, M., & Krejčí, L. (2018) · Nucleic Acids Research · doi:10.1093/nar/gky111 · Cited by 48 · see citing works
Kuwabara, N., Murayama, Y., Hashimoto, H., 小甲, 裕., Ikeguchi, M., Sato, M., Mayanagi, K., Tsutsui, Y., Iwasaki, H., & Shimizu, T. (2012) · Structure · doi:10.1016/j.str.2012.01.005 · Cited by 46 · see citing works
Investigations of homologous recombination pathways and their regulation.
Daley, J. M., Kwon, Y., Niu, H., & Sung, P. (2013) · Europe PMC (PubMed Central) · Cited by 46 · see citing works
Self-citationDirect observation of twisting steps during Rad51 polymerization on DNA
Arata, H. F., Dupont, A., Miné-Hattab, J., Disseau, L., Renodon‐Cornière, A., Takahashi, M., Viovy, J., & Cappello, G. (2009) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0902234106 · Cited by 44 · see citing works
Dss1 Interaction with Brh2 as a Regulatory Mechanism for Recombinational Repair
Zhou, Q., Kojić, M., Cao, Z., Lisby, M., Mazloum, N. A., & Holloman, W. K. (2007) · Molecular and Cellular Biology · doi:10.1128/mcb.01907-06 · Cited by 45 · see citing works
Multiple Regulation of Rad51-Mediated Homologous Recombination by Fission Yeast Fbh1
Tsutsui, Y., Kurokawa, Y., Ito, K., Siddique, M. S. P., Kawano, Y., Yamao, F., & Iwasaki, H. (2014) · PLoS Genetics · doi:10.1371/journal.pgen.1004542 · Cited by 44 · see citing works
Depletion of DSS1 protein disables homologous recombinational repair in human cells
Kristensen, C. N., Bystol, K., Li, B., Serrano, L., & Brenneman, M. A. (2010) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/j.mrfmmm.2010.08.007 · Cited by 43 · see citing works
Li, B. (2015) · Eukaryotic Cell · doi:10.1128/ec.00207-14 · Cited by 39 · see citing works
Cole, D. J., Rajendra, E., Roberts-Thomson, M., Hardwick, B. S., McKenzie, G. J., Payne, M. C., Venkitaraman, A. R., & Skylaris, C. (2011) · PLoS Computational Biology · doi:10.1371/journal.pcbi.1002096 · Cited by 37 · see citing works
Exploring the roles of PALB2 at the crossroads of DNA repair and cancer
Pauty, J., Rodrigue, A., Couturier, A. M., Buisson, R., & Masson, J. (2014) · Biochemical Journal · doi:10.1042/bj20140208 · Cited by 37 · see citing works
RADX controls RAD51 filament dynamics to regulate replication fork stability
Adolph, M. B., Mohamed, T. M., Balakrishnan, S., Xue, C., Morati, F., Modesti, M., Greene, E. C., Chazin, W., & Cortez, D. (2021) · Molecular Cell · doi:10.1016/j.molcel.2020.12.036 · Cited by 34 · see citing works
Regulation of the DNA Damage Response to DSBs by Post-Translational Modifications
Oberle, C., & Blattner, C. (2010) · Current Genomics · doi:10.2174/138920210791110979 · Cited by 36 · see citing works
DNA Binding, Annealing, and Strand Exchange Activities of Brh2 Protein from Ustilago maydis
Mazloum, N. A., Zhou, Q., & Holloman, W. K. (2007) · Biochemistry · doi:10.1021/bi700399m · Cited by 36 · see citing works
Molding BRCA2 function through its interacting partners
Martinez, J. S., Baldeyron, C., & Carreira, A. (2015) · Cell Cycle · doi:10.1080/15384101.2015.1093702 · Cited by 32 · see citing works
Willis, N. A., Panday, A., Duffey, E. E., & Scully, R. (2018) · PLoS Genetics · doi:10.1371/journal.pgen.1007486 · Cited by 29 · see citing works
Cancer-Associated Mutations in BRC Domains of BRCA2 Affect Homologous Recombination Induced by Rad51
Tal, A., Arbel‐Goren, R., & Stavans, J. (2009) · Journal of Molecular Biology · doi:10.1016/j.jmb.2009.09.011 · Cited by 33 · see citing works
Zhou, Q., Kojić, M., & Holloman, W. K. (2009) · Journal of Biological Chemistry · doi:10.1074/jbc.m809226200 · Cited by 30 · see citing works
Say, A. F., Ledford, L. L., Sharma, D., Singh, A. K., Leung, W., Sehorn, H. A., Tsubouchi, H., Sung, P., & Sehorn, M. G. (2011) · DNA repair · doi:10.1016/j.dnarep.2011.03.006 · Cited by 29 · see citing works
Self-citationManthey, G. M., & Bailis, A. M. (2010) · PLoS ONE · doi:10.1371/journal.pone.0011889 · Cited by 29 · see citing works
Functional attributes of the Saccharomyces cerevisiae meiotic recombinase Dmc1
Busygina, V., Gaines, W. A., Xu, Y., Kwon, Y., Williams, G. J., Lin, S., Chang, H.-Y., Chi, P., Wang, H. W., & Sung, P. (2013) · DNA repair · doi:10.1016/j.dnarep.2013.05.004 · Cited by 27 · see citing works
Self-citationSecond-End Capture in DNA Double-Strand Break Repair Promoted by Brh2 Protein of Ustilago maydis
Mazloum, N. A., & Holloman, W. K. (2009) · Molecular Cell · doi:10.1016/j.molcel.2008.12.023 · Cited by 27 · see citing works
Biochemistry of Eukaryotic Homologous Recombination
Heyer, W. (2006) · Springer eBooks · doi:10.1007/978-3-540-71021-9_4 · Cited by 38 · see citing works
Marple, T. C., Kim, T. M., & Hasty, P. (2006) · Mutation research. Fundamental and molecular mechanisms of mutagenesis · doi:10.1016/j.mrfmmm.2006.08.005 · Cited by 24 · see citing works
Taking a Bad Turn: Compromised DNA Damage Response in Leukemia
Nilles, N., & Fahrenkrog, B. (2017) · Cells · doi:10.3390/cells6020011 · Cited by 22 · see citing works
Xu, Z., Xie, H., Song, L., Huang, Y., & Huang, J. (2025) · DNA repair · doi:10.1016/j.dnarep.2025.103847 · Cited by 24 · see citing works
BRCA2 BRC missense variants disrupt RAD51-dependent DNA repair
Jiménez-Sáinz, J., Mathew, J., Moore, G., Lahiri, S., Garbarino, J. M., Eder, J. P., Rothenberg, E., & Jensen, R. B. (2022) · eLife · doi:10.7554/elife.79183 · Cited by 19 · see citing works
Barbour, A., & Wuttke, D. (2023) · Current Opinion in Structural Biology · doi:10.1016/j.sbi.2023.102611 · Cited by 14 · see citing works
High Levels of Wild-Type BRCA2 Suppress Homologous Recombination
Magwood, A. C., Mundia, M. M., & Baker, M. D. (2012) · Journal of Molecular Biology · doi:10.1016/j.jmb.2012.05.007 · Cited by 18 · see citing works
Role of the RAD51–SWI5–SFR1 Ensemble in homologous recombination
Su, G.-C., Yeh, H., Lin, S., Chung, C.-I., Huang, Y.-S., Liu, Y., Lyu, P. C., & Chi, P. (2016) · Nucleic Acids Research · doi:10.1093/nar/gkw375 · Cited by 16 · see citing works
Self-citationMutagenic Consequences of Sublethal Cell Death Signaling
Hawkins, C. J., & Miles, M. A. (2021) · International Journal of Molecular Sciences · doi:10.3390/ijms22116144 · Cited by 15 · see citing works
Moore, D. M., Karlin, J., González‐Barrera, S., Mardiros, A., Lisby, M., Doughty, A. T. B., Gilley, J. A., Rothstein, R. J., Friedberg, E. C., & Fischhaber, P. L. (2009) · Nucleic Acids Research · doi:10.1093/nar/gkp709 · Cited by 15 · see citing works
BRCA2: one small step for DNA repair, one giant protein purified.
Jensen, R. B. (2013) · Europe PMC (PubMed Central) · Cited by 14 · see citing works
Altered DNA Binding by the Human Rad51-R150Q Mutant Found in Breast Cancer Patients
Ishida, T., Takizawa, Y., Sakane, I., & Kurumizaka, H. (2007) · Biological and Pharmaceutical Bulletin · doi:10.1248/bpb.30.1374 · Cited by 14 · see citing works
Multiple interlinked mechanisms to circumvent DNA replication roadblocks
Eppink, B., Wyman, C., & Kanaar, R. (2006) · Experimental Cell Research · doi:10.1016/j.yexcr.2006.06.011 · Cited by 14 · see citing works
Thakur, R. S., Basavaraju, S., Khanduja, J. S., Muniyappa, K., & Nagaraju, G. (2015) · Journal of Biological Chemistry · doi:10.1074/jbc.m115.671164 · Cited by 12 · see citing works
Homologous recombination defects and how they affect replication fork maintenance
Son, M. Y., & Hasty, P. (2018) · Global Medical Genetics · doi:10.3934/genet.2018.4.192 · Cited by 12 · see citing works
Enhancement of the RAD51 Recombinase Activity by the Tumor Suppressor PALB2
Dray, E. V., Etchin, J., Wiese, C., Saro, D., Williams, G. J., Hammel, M., Yu, X., Galkin, V. E., Liu, D., Tsai, M.-S., et al. (2010) · doi:10.2172/993859 · Cited by 12 · see citing works
Self-citationKo, J. H., Son, M. Y., Zhou, Q., Molnárová, L., Song, L., Mlčoušková, J., Je̅kabsons, A., Montagna, C., Krejčí, L., & Hasty, P. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.108543 · Cited by 11 · see citing works
Špı́rek, M., Taylor, M. R. G., Beláň, O., Boulton, S. J., & Krejčí, L. (2021) · Nature Communications · doi:10.1038/s41467-021-25830-x · Cited by 9 · see citing works
Assembling an orchestra: Fanconi anemia pathway of repair
Yuan, F. (2010) · Frontiers in bioscience · doi:10.2741/3666 · Cited by 10 · see citing works
Biochemical Studies on Human Rad51-Mediated Homologous Recombination
Kwon, Y., Zhao, W., & Sung, P. (2011) · Methods in molecular biology · doi:10.1007/978-1-61779-129-1_24 · Cited by 8 · see citing works
Self-citation
- Authors
- Filippo, J. S., Chi, P., Sehorn, M. G., Etchin, J., Krejci, L., & Sung, P.
- Affiliations
- Yale University (US)
- Corresponding author
- Sung, Patrick
- Venue
- Journal of Biological Chemistry
- Publisher
- Elsevier BV
- Published
- 2006-04
- Volume
- 281
- Issue
- 17
- Pages
- 11649-11657
- ISSN
- 0021-9258 (ISSN-L)
- PMC
- PMC2077811
- OpenAlex
- W2027230427
- Cited by
- 134
- References
- 32
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 2 · 2023: 5 · 2022: 3 · 2021: 7 · 2020: 3 · 2019: 1 · 2018: 7 · 2017: 4 · 2016: 6 · 2015: 5 · 2014: 3 · 2013: 5 · 2012: 6
- FWCI
- 6.25
- Type
- journal article
- Language
- en
- Open access
- hybrid
- doi.org
- Licence
- Locations
- Journal of Biological Chemistry (publishedVersion, cc-by)
- PubMed (publishedVersion)
- PubMed Central (acceptedVersion)
- Funders
- U.S. Department of Defense
- Wellcome Trust (GR076476, 076476)
- National Institutes of Health
- University of California, Irvine
- National Cancer Institute (R01CA110415)
- National Institute of Environmental Health Sciences (R01ES007061)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › DNA Repair Mechanisms
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › BRCA gene mutations in cancer
- Health Sciences › Medicine › Oncology › PARP inhibition in cancer therapy
- Keywords
- BRCA2, RAD51, homology-directed repair, DNA recombination, DNA-binding domain, single-stranded DNA, replication protein A, tumor suppressor function, DNA repair, protein-DNA interactions
- MeSH terms
- DNA Damage, DNA Repair, DNA, Single-Stranded, Humans, Peptide Fragments, Recombination, Genetic, DNA Primers, BRCA2 Protein, Rad51 Recombinase, Replication Protein A
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