Brominated Furanones Inhibit Biofilm Formation by Salmonella enterica Serovar Typhimurium
Open accessAbstract
Salmonella enterica serovar Typhimurium is a main cause of bacterial food-borne diseases. As Salmonella can form biofilms in which it is better protected against antimicrobial agents on a wide diversity of surfaces, it is of interest to explore ways to inhibit biofilm formation. Brominated furanones, originally extracted from the marine alga Delisea pulchra, are known to interfere with biofilm formation in several pathogens. In this study, we have synthesized a small focused library of brominated furanones and tested their activity against S. enterica serovar Typhimurium biofilm formation. We show that several furanones inhibit Salmonella biofilm formation at non-growth-inhibiting concentrations. The most interesting compounds are (Z)-4-bromo-5-(bromomethylene)-3-alkyl-2(5H)-furanones with chain lengths of two to six carbon atoms. A microarray study was performed to analyze the gene expression profiles of Salmonella in the presence of (Z)-4-bromo-5-(bromomethylene)-3-ethyl-2(5H)-furanone. The induced genes include genes that are involved in metabolism, stress response, and drug sensitivity. Most of the repressed genes are involved in metabolism, the type III secretion system, and flagellar biosynthesis. Follow-up experiments confirmed that this furanone interferes with the synthesis of flagella by Salmonella. No evidence was found that furanones act on the currently known quorum-sensing systems in Salmonella. Interestingly, pretreatment with furanones rendered Salmonella biofilms more susceptible to antibiotic treatment. Conclusively, this work demonstrates that particular brominated furanones have potential in the prevention of biofilm formation by Salmonella serovar Typhimurium.
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Brominated furanones obtained by extraction from the red algae Delisea pulchra interfere in Salmonella enterica biofilm formation.
“In that study, it was observed that brominated furanones obtained by extraction from the red algae Delisea pulchra, interfere in this biofilm formation, but more studies are needed to determine a long term efficiency [41].”
From The Potential of Seaweeds as a Source of Functional Ingredients of Prebiotic and Antioxidant Value (2019) · cites this paper as [41]
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- Authors
- Janssens, J. C. A., Steenackers, H., Robijns, S., Gellens, E., Levin, J., Zhao, H., Hermans, K., De Coster, D., Verhoeven, T. L., Marchal, K., Vanderleyden, J., De Vos, D. E., & De Keersmaecker, S. C. J.
- Affiliations
- Centre of Microbial and Plant Genetics, Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B-3001 Leuven, Belgium
- KU Leuven (BE)
- Centre for Surface Chemistry and Catalysis, Katholieke Universiteit Leuven, Kasteelpark Arenberg 23, B-3001 Leuven, Belgium
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2008-11
- Volume
- 74
- Issue
- 21
- Pages
- 6639-6648
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC2576703
- OpenAlex
- W2107140594
- Cited by
- 223
- References
- 70
- Citations by year
- 2026: 5 · 2025: 17 · 2024: 6 · 2023: 11 · 2022: 7 · 2021: 7 · 2020: 23 · 2019: 17 · 2018: 16 · 2017: 17 · 2016: 15 · 2015: 13 · 2014: 17 · 2013: 8 · 2012: 13
- FWCI
- 4.25
- Type
- journal article
- Language
- en
- Open access
- green
- www.vliz.be
- Licence
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Flanders Marine Institute (Flanders Marine Institute) (publishedVersion, other-oa)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Ghent University Academic Bibliography (Ghent University) (submittedVersion, other-oa)
- Lirias (submittedVersion)
- Funders
- Ohio State University
- Fonds Wetenschappelijk Onderzoek
- KU Leuven
- Vlaamse regering
- School of Medicine, Stanford University
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Physical Sciences › Engineering › Ocean Engineering › Marine Biology and Environmental Chemistry
- Physical Sciences › Chemistry › Organic Chemistry › Antimicrobial agents and applications
- Keywords
- brominated furanones, Salmonella typhimurium, biofilm inhibition, Delisea pulchra, gene expression profiling, flagellar biosynthesis, type III secretion system, quorum sensing, antibiotic susceptibility, foodborne pathogens
- MeSH terms
- Anti-Bacterial Agents, Flagella, Furans, Locomotion, Microbial Sensitivity Tests, Salmonella typhimurium, Molecular Structure, Gene Expression Regulation, Bacterial, Biofilms, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Microbial Viability
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