The DOI matches its Crossref record; 4 of the 4 references it cites were found in a registry or an index.
Advances and Challenges in Liquid Chromatography-Mass Spectrometry-based Proteomics Profiling for Clinical Applications
Open accessAbstract
Recent advances in proteomics technologies provide tremendous opportunities for biomarker-related clinical applications; however, the distinctive characteristics of human biofluids such as the high dynamic range in protein abundances and extreme complexity of the proteomes present tremendous challenges. In this review we summarize recent advances in LC-MS-based proteomics profiling and its applications in clinical proteomics as well as discuss the major challenges associated with implementing these technologies for more effective candidate biomarker discovery. Developments in immunoaffinity depletion and various fractionation approaches in combination with substantial improvements in LC-MS platforms have enabled the plasma proteome to be profiled with considerably greater dynamic range of coverage, allowing many proteins at low ng/ml levels to be confidently identified. Despite these significant advances and efforts, major challenges associated with the dynamic range of measurements and extent of proteome coverage, confidence of peptide/protein identifications, quantitation accuracy, analysis throughput, and the robustness of present instrumentation must be addressed before a proteomics profiling platform suitable for efficient clinical applications can be routinely implemented.
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Mass spectrometry-based proteomics
Aebersold, R., & Mann, M. (2003) · Nature · doi:10.1038/nature01511 · Cited by 6,955
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Quantitative analysis of complex protein mixtures using isotope-coded affinity tags
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Zhang, Q., Ames, J. M., Smith, R. D., Baynes, J. W., & Metz, T. O. (2009) · Journal of Proteome Research · doi:10.1021/pr800858h · Cited by 421
Self-citationSignificance Analysis of Spectral Count Data in Label-free Shotgun Proteomics
Choi, H., Fermin, D., & Nesvizhskii, A. I. (2008) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m800203-mcp200 · Cited by 364
High-sensitivity Analytical Approaches for the Structural Characterization of Glycoproteins
Alley, W. R., Mann, B. F., & Novotny, M. V. (2013) · Chemical Reviews · doi:10.1021/cr3003714 · Cited by 310
Ionization and transmission efficiency in an electrospray ionization—mass spectrometry interface
Page, J. S., Kelly, R. T., Tang, K., & Smith, R. D. (2007) · Journal of the American Society for Mass Spectrometry · doi:10.1016/j.jasms.2007.05.018 · Cited by 265
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Metadata
- Authors
- Qian, W.-J., Jacobs, J. M., Liu, T., Camp, D. G., & Smith, R. D.
- Venue
- Molecular & Cellular Proteomics
- Publisher
- Elsevier BV
- Published
- 2006-10
- Volume
- 5
- Issue
- 10
- Pages
- 1727-1744
- ISSN
- 1535-9476 (ISSN-L)
- OpenAlex
- W2121863513
- Cited by
- 362
- References
- 4
- FWCI
- 15.68
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Qian, W.-J., Jacobs, J. M., Liu, T., Camp, D. G., & Smith, R. D. (2006). Advances and Challenges in Liquid Chromatography-Mass Spectrometry-based Proteomics Profiling for Clinical Applications. Molecular & Cellular Proteomics. https://doi.org/10.1074/mcp.m600162-mcp200
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