The DOI matches its Crossref record; 34 of the 34 references it cites were found in a registry or an index.
Isotope-labeled Protein Standards
Open accessAbstract
Diagnostic development and public health surveillance require technologies that provide specific identification and absolute quantification of protein biomarkers. Beside immunologically related techniques (e.g. enzyme-linked immunosorbent assay), MS is gaining increasing interest due to its high sensitivity and specificity. Furthermore, MS-based analyses are extremely accurate quantitatively, provided that suitable reference standards are available. Recently, the use of chemically synthesized isotope-labeled marker peptides for MS-based absolute quantification of proteins has led to major advances. However, we show here that the use of such peptides can lead to severe biases. In this work, we present an innovative strategy (Protein Standard Absolute Quantification) that uses in vitro-synthesized isotope-labeled full-length proteins as standards for absolute quantification. As those protein standards perfectly match the biochemical properties of the target proteins, they can be directly added into the samples to be analyzed, allowing a highly accurate quantification of proteins even in prefractionated complex samples. The power of our Protein Standard Absolute Quantification methodology for accurate absolute quantification of biomarkers was demonstrated both on water and urine samples contaminated with Staphylococcus aureus superantigenic toxins as typical biomarkers of public health interest.
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Citations
Cites
Mass Spectrometry and Protein Analysis
Domon, B., & Aebersold, R. (2006) · Science · doi:10.1126/science.1124619 · Cited by 2,181
Protein biomarker discovery and validation: the long and uncertain path to clinical utility
Rifai, N., Gillette, M. A., & Carr, S. A. (2006) · Nature Biotechnology · doi:10.1038/nbt1235 · Cited by 1,867
Absolute quantification of proteins and phosphoproteins from cell lysates by tandem MS
Gerber, S. A., Rush, J., Stemman, O., Kirschner, M. W., & Gygi, S. P. (2003) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.0832254100 · Cited by 1,733
Mass spectrometry–based proteomics turns quantitative
Ong, S.-E., & Mann, M. (2005) · Nature Chemical Biology · doi:10.1038/nchembio736 · Cited by 1,572 · cited 1×
“To gain in measurement accuracy, absolute quantitative methodologies still require an internal standardization via stable isotope dilution strategies [6, 24].”
Quantitative mass spectrometric multiple reaction monitoring assays for major plasma proteins
Anderson, L., & Hunter, C. L. (2006) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m500331-mcp200 · Cited by 1,230
Lu, P., Vogel, C., Wang, R., Yao, X., & Marcotte, E. M. (2007) · Nature Biotechnology · doi:10.1038/nbt1270 · Cited by 1,139
Exotoxins of Staphylococcus aureus
Dinges, M. M., Orwin, P. M., & Schlievert, P. M. (2000) · Clinical Microbiology Reviews · doi:10.1128/cmr.13.1.16 · Cited by 967
Toxic Shock Syndrome and Bacterial Superantigens: An Update
McCormick, J. K., Yarwood, J. M., & Schlievert, P. M. (2001) · Annual Review of Microbiology · doi:10.1146/annurev.micro.55.1.77 · Cited by 785
Computational prediction of proteotypic peptides for quantitative proteomics
Mallick, P., Schirle, M., Chen, S. S., Flory, M. R., Lee, H., Martin, D., Ranish, J., Raught, B., Schmitt, R., Werner, T., et al. (2007) · Nature Biotechnology · doi:10.1038/nbt1275 · Cited by 695
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Cited by
Protein Analysis by Shotgun/Bottom-up Proteomics
Zhang, Y., Fonslow, B. R., Shan, B., Baek, M.-C., & Yates, J. R. (2013) · Chemical Reviews · doi:10.1021/cr3003533 · Cited by 1,491
Quantitative mass spectrometry in proteomics: critical review update from 2007 to the present
Bantscheff, M., Lemeer, S., Savitski, M. M., & Kuster, B. (2012) · Analytical and Bioanalytical Chemistry · doi:10.1007/s00216-012-6203-4 · Cited by 801
Carr, S. A., Abbatiello, S. E., Ackermann, B. L., Borchers, C., Domon, B., Deutsch, E. W., Grant, R. P., Hoofnagle, A. N., Hüttenhain, R., Koomen, J. M., et al. (2014) · Molecular & Cellular Proteomics · doi:10.1074/mcp.m113.036095 · Cited by 561
Rauniyar, N. (2015) · International Journal of Molecular Sciences · doi:10.3390/ijms161226120 · Cited by 406
Hoshi, Y., Uchida, Y., Tachikawa, M., Inoue, T., Ohtsuki, S., & Terasaki, T. (2013) · Journal of Pharmaceutical Sciences · doi:10.1002/jps.23575 · Cited by 254
Metadata
- Authors
- Brun, V., Dupuis, A., Adrait, A., Marcellin, M., Thomas, D., Court, M., Vandenesch, F., & Garin, J.
- Venue
- Molecular & Cellular Proteomics
- Publisher
- Elsevier BV
- Published
- 2007-12
- Volume
- 6
- Issue
- 12
- Pages
- 2139-2149
- ISSN
- 1535-9476 (ISSN-L)
- OpenAlex
- W2171081285
- Cited by
- 445
- References
- 34
- FWCI
- 10.60
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Brun, V., Dupuis, A., Adrait, A., Marcellin, M., Thomas, D., Court, M., Vandenesch, F., & Garin, J. (2007). Isotope-labeled Protein Standards. Molecular & Cellular Proteomics. https://doi.org/10.1074/mcp.m700163-mcp200
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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