Serological assays on eight cases of human dirofilariasis identified by morphology and DNA diagnostics
Abstract
Specimens of Dirofilaria sp. removed from eight Italian patients were identified as D. repens by morphology and confirmed as such by a PCR-based method of DNA analysis. Blood samples were also drawn from the patients so that two serological tests (ELISA and western blot), one based on the recognition of molecular markers recently identified in the somatic antigenic complex of D. repens, could be evaluated. The antigenic complex used in the ELISA only gave a weak sensitivity. However, the western-blot assays, based on the polypeptide molecular markers, were found to have greater sensitivity and should be useful in detecting human cases of dirofilariasis.
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There is a good correlation between PCR-based and serology-based results, as evidenced by a study of eight cases with subcutaneous nodules or ocular localization analyzed in parallel by molecular techniques, ELISA, and Western blotting.
“Finally, there is a good correlation between PCR-based and serologybased results, as evidenced by a study of eight cases with subcutaneous nodules or ocular localization that were analyzed in parallel by using molecular techniques, ELISA, and Western blotting (78).”
From Human and animal dirofilariasis: The emergence of a zoonotic mosaic (2012) · cites this paper as [78]
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- Authors
- Cancrini, G., Prieto, G., Via, G. F., Giannetto, S., Trincali, R., Pietrobelli, M., & Simon, F.
- Affiliations
- Sapienza University of Rome (IT)
- Universidad de Salamanca (ES)
- University of Messina (IT)
- University of Padua (IT)
- Corresponding author
- Cancrini, G.
- Venue
- Annals of Tropical Medicine & Parasitology
- Publisher
- Informa UK Limited
- Published
- 1999-03
- Volume
- 93
- Issue
- 2
- Pages
- 147-152
- ISSN
- 0003-4983 (ISSN-L)
- 1364-8594
- OpenAlex
- W2005489247
- Cited by
- 23
- References
- 13
- Citations by year
- 2024: 3 · 2020: 2 · 2015: 2 · 2014: 4 · 2013: 2 · 2012: 2
- FWCI
- 0.00
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Annals of Tropical Medicine and Parasitology (publishedVersion)
- PubMed (publishedVersion)
- IRIS Research product catalog (Sapienza University of Rome) (submittedVersion)
- Topics
- Health Sciences › Medicine › Infectious Diseases › Parasitic Diseases Research and Treatment
- Health Sciences › Medicine › Public Health, Environmental and Occupational Health › Research on Leishmaniasis Studies
- Life Sciences › Immunology and Microbiology › Parasitology › Parasites and Host Interactions
- Keywords
- human dirofilariasis, Dirofilaria repens, serological diagnosis, ELISA, Western blot, molecular markers, diagnostic sensitivity, DNA-based species identification
- MeSH terms
- Animals, Antibodies, Helminth, Dirofilaria, Dirofilariasis, Enzyme-Linked Immunosorbent Assay, Female, Humans, Male, Parasitology, Sex Characteristics, Blotting, Western, Polymerase Chain Reaction, DNA, Helminth
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