Cited by 61Cell death mechanisms and regulationVitamin C and Antioxidants ResearchBioactive Compounds and Antitumor Agents

Ultrastructural Aspects of Autoschizis: A New Cancer Cell Death Induced by the Synergistic Action of Ascorbate/Menadione on Human Bladder Carcinoma Cells

Gilloteaux, Jacques, Jamison, James M., Arnold, David, Taper, Henryk S., Summers, Jack L.·2001·Ultrastructural Pathology

Abstract

Scanning and transmission electron microscopy were employed to further characterize the cytotoxic effects of a ascorbic acid/menadione (or vitamin C/vitamin K3) combination on a human bladder carcinoma T24 cell line. Following 1-h treatment T24 cells display membrane and mitochondrial defects as well as excision of cytoplasmic fragments that contain no organelles. These continuous self-excisions reduce the cell size. Concomitant, nuclear changes, chromatin disassembly, nucleolar condensation and fragmentation, and decreased nuclear volume lead to cell death via a process similar to karyorrhexis and karyolysis. Because this cell death is achieved through a progressive loss of cytoplasm due to self-morsellation, the authors named this mode of cell death autoschizis (from the Greek autos, self, and schizein, to split, as defined in Scanning. 1998; 20: 564-575). This morphological characterization of autoschizic cell death confirms and extends the authors previous reports and demonstrates that this cell death is distinct from apoptosis.

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Crossref · 10.1080/019131201300343810

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Authors
Gilloteaux, J., Jamison, J. M., Arnold, D., Taper, H. S., & Summers, J. L.
Affiliations
  • Lake Erie College of Osteopathic Medicine (US)
  • Summa Health System (US)
  • Pennsylvania State University (US)
  • UCLouvain (BE)
Venue
Ultrastructural Pathology
Published
2001-01
Volume
25
Issue
3
Pages
183-192
ISSN
  • 0191-3123 (ISSN-L)
  • 1521-0758
OpenAlex
W2168327756
Cited by
61
References
32
Citations by year
2026: 1 · 2025: 3 · 2023: 2 · 2022: 2 · 2021: 1 · 2020: 6 · 2018: 4 · 2017: 2 · 2016: 2 · 2015: 4 · 2014: 3 · 2013: 3
FWCI
1.51
Type
journal article
Language
en
Open access
closed
Locations
  • Ultrastructural Pathology (publishedVersion)
  • PubMed (publishedVersion)
  • Digital Access to Libraries (submittedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Cell death mechanisms and regulation
  • Health Sciences › Nursing › Nutrition and Dietetics › Vitamin C and Antioxidants Research
  • Life Sciences › Pharmacology, Toxicology and Pharmaceutics › Toxicology › Bioactive Compounds and Antitumor Agents
Keywords
cancer cell death, vitamin C, menadione, T24 cells, mitochondrial defects, membrane damage, karyorrhexis, karyolysis, nuclear fragmentation, cytoplasmic fragmentation, non-apoptotic cell death, synergistic cytotoxicity
MeSH terms
Ascorbic Acid, Urinary Bladder Neoplasms, Carcinoma, Drug Synergism, Humans, Microscopy, Electron, Microscopy, Electron, Scanning, Tumor Cells, Cultured, Cell Death, Vitamin K 3

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