MiR-9-5p inhibits mitochondrial damage and oxidative stress in AD cell models by targeting GSK-3β
Abstract
This study aims to investigate the effects and underlying mechanisms of overexpression microRNA-9-5p (miR-9-5p) on the Aβ-induced mouse hippocampal neuron cell line HT22. Different concentrations of Aβ25-35 (10, 20, 40, 80, and 160 μM) treatment were used to establish AD model in HT22 cells. The CCK-8 assay was used to measure the cell viability. The mRNA expression levels of miR-9-5p and glycogen synthase kinase-3β (GSK-3β) were determined by RT-qPCR. HT22 cell apoptosis was analyzed flow cytometry. MiR-9-5p was down-regulated in Aβ25-35-induced HT22 cells. GSK-3β is a functional target for miR-9-5p. MiR-9-5p overexpression inhibited Aβ25-35-induced mitochondrial dysfunction, cell apoptosis, and oxidative stress by regulating GSK-3β expression in HT22 cells. Furthermore, through targeting GSK-3β, overexpression of miR-9-5p partly activated nuclear factor Nrf2/Keap1 signaling, including part increases of Nrf2, HO-1, SOD-1, GCLC expression and slight decrease of Keap1 expression. Our results showed miR-9-5p may play a powerful role in the pathogenesis of AD.
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miR-9 regulates NSC proliferation, differentiation, and neuronal integration, with context-dependent functional effects in AD-relevant contexts.
“Among these, miR-9 is one of the more extensively studied regulators of NSC proliferation, differentiation, and neuronal integration, and it displays context-dependent functional effects in AD-relevant contexts (Chang et al., 2014; Liu et al., 2020; Roese-Koerner et al., 2017; Xie et al., 2017).”
From Cell-specific MicroRNA networks orchestrate the pathogenesis of Alzheimer’s disease (2026) · cites this paper as Liu et al., 2020
In Aβ-induced neuronal stress models, miR-9-5p overexpression protects against mitochondrial dysfunction, oxidative damage, and apoptosis by targeting GSK3β.
“Furthermore, in Aβ-induced neuronal stress models, miR-9-5p overexpression protects against mitochondrial dysfunction, oxidative damage, and apoptosis by targeting GSK3β, supporting a pro-survival role under amyloidogenic conditions (Liu et al., 2020).”
From Cell-specific MicroRNA networks orchestrate the pathogenesis of Alzheimer’s disease (2026) · cites this paper as Liu et al., 2020
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MicroRNAs in Extracellular Vesicles of Alzheimer’s Disease
Li, W., & Zheng, Y. (2023) · Cells · doi:10.3390/cells12101378 · Cited by 43 · see citing works
Cell-specific MicroRNA networks orchestrate the pathogenesis of Alzheimer’s disease
Donepudi, K., Eadha, S., Rodarte, D., Sharma, B., & Kumar, S. (2026) · Ageing Research Reviews · doi:10.1016/j.arr.2026.103211
- Authors
- Liu, J., Zuo, X., Han, J., Dai, Q., Xu, H., Liu, Y., & Cui, S.
- Affiliations
- Department of Geriatrics, Affiliated Hospital of Qinghai University, Xining, China
- Department of Hematology, Affiliated Hospital of Qinghai University, Xining, China
- Venue
- Bioscience, Biotechnology, and Biochemistry
- Publisher
- Informa UK Limited
- Published
- 2020-11-01
- Volume
- 84
- Issue
- 11
- Pages
- 2273-2280
- ISSN
- 1347-6947
- 0916-8451
- Cited by
- 47
- References
- 7
- Language
- en
- Licence
- academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model (vor)
- Funders
- Science and Technology Innovation Ability Promotion Project of Qinghai Province of China (No.2017-ZJ-771)
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