The DOI matches its Crossref record; 12 of the 12 references it cites were found in a registry or an index.
Expression of phosphorylated histone H2AX in cultured cell lines following exposure to X‐rays
Abstract
PURPOSE: Exposure to ionizing radiation results in phosphorylation of histone H2AX (gammaH2AX) at sites of DNA double-strand breaks. To determine the relationship between gammaH2AX formation and radiosensitivity, the rate of formation and loss of gammaH2AX were examined in several cultured cell lines following exposure to 253 kV X-rays. MATERIALS AND METHODS: Flow and image cytometry were both performed using a mouse monoclonal antibody against gammaH2AX. Immunoblotting was used to confirm cell line-dependent differences in antibody staining. Cell lines examined included V79 and CHO-K1 hamster cells, the human tumour cell lines SiHa, WiDr, DU145, WIL-2NS, HT144, HCC1937 and U87, and the normal cell strain HFL1. Radiosensitivity was measured using a standard clonogenic assay. RESULTS: Using flow cytometry, gammaH2AX formation was detected 1 h after doses as low as 20 cGy. Peak levels of gammaH2AX were observed within 15-30 min after irradiation and both the rate of radiation-induced gammaH2AX formation and loss were cell type dependent. Maximum levels of gammaH2AX formation were lower for HT144 cells mutant for the ataxia telangiectasia gene. Half-times of loss after irradiation ranged from 1.6 to 7.2 h and were associated with a decrease in the total number of foci per cell. The half-time of loss of gammaH2AX was correlated with clonogenic survival for 10 cell lines (r2=0.66). CONCLUSIONS: GammaH2AX can be detected with excellent sensitivity using both flow and image analysis. The rate of gammaH2AX loss may be an important factor in the response of cells to ionizing radiation, with more rapid loss and less retention associated with more radioresistant cell lines.
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Cites
DNA double-stranded breaks induce histone H2AX phosphorylation on serine 139
Rogakou, E. P., Pilch, D. R., Orr, A. H., Ivanova, V. S., & Bonner, W. M. (1998) · Journal of Biological Chemistry · doi:10.1074/jbc.273.10.5858 · Cited by 5,438
Megabase Chromatin Domains Involved in DNA Double-Strand Breaks in Vivo
Rogakou, E. P., Boon, C., Redon, C., & Bonner, W. M. (1999) · The Journal of Cell Biology · doi:10.1083/jcb.146.5.905 · Cited by 2,422
A critical role for histone H2AX in recruitment of repair factors to nuclear foci after DNA damage
Paull, T. T., Rogakou, E. P., Yamazaki, V., Kirchgessner, C. U., Gellert, M., & Bonner, W. M. (2000) · Current Biology · doi:10.1016/s0960-9822(00)00610-2 · Cited by 2,098
ATM Phosphorylates Histone H2AX in Response to DNA Double-strand Breaks
Burma, S., Chen, B. P., Murphy, M., Kurimasa, A., & Chen, D. J. (2001) · Journal of Biological Chemistry · doi:10.1074/jbc.c100466200 · Cited by 1,954
Genomic Instability in Mice Lacking Histone H2AX
Celeste, A., Petersen, S., Romanienko, P. J., Fernandez-Capetillo, O., Chen, H. T., Sedelnikova, O. A., Reina-San-Martin, B., Coppola, V., Meffre, E., Difilippantonio, M. J., et al. (2002) · Science · doi:10.1126/science.1069398 · Cited by 1,385
Histone H2A variants H2AX and H2AZ
Redon, C., Pilch, D., Rogakou, E., Sedelnikova, O., Newrock, K., & Bonner, W. (2002) · Current Opinion in Genetics & Development · doi:10.1016/s0959-437x(02)00282-4 · Cited by 767
Increased ionizing radiation sensitivity and genomic instability in the absence of histone H2AX
Bassing, C. H., Chua, K. F., Sekiguchi, J., Suh, H., Whitlow, S. R., Fleming, J. C., Monroe, B. C., Ciccone, D. N., Yan, C., Vlasakova, K., et al. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.122228699 · Cited by 529
The Role of DNA Single- and Double-Strand Breaks in Cell Killing by Ionizing Radiation
Olive, P. L. (1998) · Radiation Research · doi:10.2307/3579807 · Cited by 345
Self-citationMacPhail, S. H., Banáth, J. P., Yu, Y., Chu, E., & Olive, P. L. (2003) · Radiation Research · doi:10.1667/rr3003 · Cited by 226
Self-citationLöbrich, M., Kühne, M., Wetzel, J., & Rothkamm, K. (2000) · Genes Chromosomes and Cancer · Cited by 71
Cited by
-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin
Kinner, A., Wu, W., Staudt, C., & Iliakis, G. (2008) · Nucleic Acids Research · doi:10.1093/nar/gkn550 · Cited by 1,256
Use of the γ-H2AX assay to monitor DNA damage and repair in translational cancer research
Ivashkevich, A., Redon, C. E., Nakamura, A. J., Martin, R. F., & Martin, O. A. (2012) · Cancer Letters · doi:10.1016/j.canlet.2011.12.025 · Cited by 462
(2003) · PubMed · Cited by 290
Inhibition of Histone Deacetylation: A Strategy for Tumor Radiosensitization
Camphausen, K., & Tofilon, P. J. (2007) · Journal of Clinical Oncology · doi:10.1200/jco.2007.11.6202 · Cited by 162
Palayoor, S. T., Mitchell, J. B., Cerna, D., DeGraff, W., John‐Aryankalayil, M., & Coleman, C. N. (2008) · International Journal of Cancer · doi:10.1002/ijc.23807 · Cited by 132
Metadata
- Authors
- Macphail, S. H., BanÁth, J. P., Yu, T. Y., Chu, E. H. M., Lambur, H., & Olive, P. L.
- Venue
- International Journal of Radiation Biology
- Publisher
- Informa UK Limited
- Published
- 2003-05
- Volume
- 79
- Issue
- 5
- Pages
- 351-359
- ISSN
- 0955-3002 (ISSN-L)
- 1362-3095
- OpenAlex
- W2046165135
- Cited by
- 317
- References
- 12
- FWCI
- 14.48
- Type
- journal article
- Language
- en
- Open access
- closed
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