The DOI matches its Crossref record; 158 of the 158 references it cites were found in a registry or an index.
-H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin
Open accessAbstract
DNA double-strand breaks (DSBs) are extremely dangerous lesions with severe consequences for cell survival and the maintenance of genomic stability. In higher eukaryotic cells, DSBs in chromatin promptly initiate the phosphorylation of the histone H2A variant, H2AX, at Serine 139 to generate gamma-H2AX. This phosphorylation event requires the activation of the phosphatidylinositol-3-OH-kinase-like family of protein kinases, DNA-PKcs, ATM, and ATR, and serves as a landing pad for the accumulation and retention of the central components of the signaling cascade initiated by DNA damage. Regions in chromatin with gamma-H2AX are conveniently detected by immunofluorescence microscopy and serve as beacons of DSBs. This has allowed the development of an assay that has proved particularly useful in the molecular analysis of the processing of DSBs. Here, we first review the role of gamma-H2AX in DNA damage response in the context of chromatin and discuss subsequently the use of this modification as a surrogate marker for mechanistic studies of DSB induction and processing. We conclude with a critical analysis of the strengths and weaknesses of the approach and present some interesting applications of the resulting methodology.
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Cites
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Cited by
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γH2AX: a sensitive molecular marker of DNA damage and repair
Mah, L.-J., El-Osta, A., & Karagiannis, T. C. (2010) · Leukemia · doi:10.1038/leu.2010.6 · Cited by 1,557
Histone H2AX Phosphorylation: A Marker for DNA Damage
Sharma, A., Singh, K., & Almasan, A. (2012) · Methods in Molecular Biology · doi:10.1007/978-1-61779-998-3_40 · Cited by 721
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Androgen-induced TOP2B-mediated double-strand breaks and prostate cancer gene rearrangements
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Metadata
- Authors
- Kinner, A., Wu, W., Staudt, C., & Iliakis, G.
- Venue
- Nucleic Acids Research
- Publisher
- Oxford University Press (OUP)
- Published
- 2008-08-21
- Volume
- 36
- Issue
- 17
- Pages
- 5678-5694
- ISSN
- 0305-1048 (ISSN-L)
- 1362-4962
- OpenAlex
- W2051193631
- Cited by
- 1,256
- References
- 158
- FWCI
- 23.82
- Type
- journal article
- Language
- en
- Open access
- gold
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- Kinner, A., Wu, W., Staudt, C., & Iliakis, G. (2008). -H2AX in recognition and signaling of DNA double-strand breaks in the context of chromatin. Nucleic Acids Research. https://doi.org/10.1093/nar/gkn550
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