Candidate K232A mutation of DGAT1 gene associated with production and reproduction traits in Indian Dairy cattle
Abstract
The present study was conducted on Hardhenu and Sahiwal cows to evaluate the genetic diversity and the effect of candidate K232A mutation of diacylglycerol O-acyltransferase (DGAT1) gene on performance traits. PCR product of 413 bp size targeting K232A mutation of DGAT 1 gene was genotyped by Eae1 restriction enzyme and further confirmed by sequencing. The PCR-RFLP analysis for K232A showed that the frequencies of alleles K and A were 0.80 and 0.20 in Hardhenu and 0.98 and 0.02 in Sahiwal cattle, respectively. The frequencies of genotypes for KK and KA genotype were estimated as 0.60 and 0.40 in Hardhenu and 0.96 and 0.04 in Sahiwal cattle, respectively. Chi-square test showed that K232A SNP did not meet with the Hardy–Weinberg equilibrium (p < .01) in Hardhenu cattle. Association analysis revealed that KA genotype was significantly associated with total milk yield (p < .01) and 305 days milk yield (p < .01)in Hardhenu cattle. Hardhenu cows having KA genotype comparatively revealed favorable mean values for AFS (880.31 ± 26.65), AFC (1166.36 ± 19.94), SP (157.46 ± 22.80), CI (441.41 ± 44.28) and AI/Conception (1.80 ± 0.17), indicating that KA genotype can be used as a candidate marker for simultaneous improvement of both production and reproduction traits.
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Individuals carrying the lysine (K) allele of DGAT1 exhibit a higher milk fat percentage, whereas those with the alanine (A) allele show higher milk protein content and total milk yield.
“Individuals carrying the lysine (K) allele exhibit a higher milk fat percentage in both Holstein and other indigenous dairy cattle breeds, whereas those with the alanine (A) allele show higher milk protein content and total milk yield [151,152,153,154].”
From The Genomic Landscape of Cattle: Domestication, Dispersal, and Adaptive Evolution (2026) · cites this paper as [154]
Taurine breeds carry a high frequency of the DGAT1 A haplotype, while zebu breeds possess a high proportion of the K haplotype.
“Haplotype analysis has shown that taurine breeds carry a high frequency of the DGAT1 A haplotype, while zebu breeds possess a high proportion of the K haplotype [154,155].”
From The Genomic Landscape of Cattle: Domestication, Dispersal, and Adaptive Evolution (2026) · cites this paper as [154]
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Effect of the DGAT1 K232A mutation and breed on milk traits in cattle populations of Ethiopia
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“Similar effects of the KK genotype on milk composition content traits, and an even greater effect of the AA and KA genotypes on average milk yield, have been observed in Holstein, Holstein crosses, Jersey and its local Kashmiri crosses, Holstein *Jersey crosses, Gir, and Kankrej cattle populations in different parts o…”
BackgroundResultInfluentialPushpa, Magotra, A., Sindhu, V., & Chaudhary, P. (2024) · Reproduction in Domestic Animals · doi:10.1111/rda.14537 · Cited by 4 · see citing works
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“…association between the bovine DGAT1 and various milk-related traits in dairy cattle (Anton et al., 2012; Ardicli et al., 2018; Bobbo et al., 2018; Gothwal et al., 2022; Grisart et al., 2002, 2004; Winter et al., 2002) Although the DGAT1 K232A marker has been widely studied in various cattle…”
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“Individuals carrying the lysine (K) allele exhibit a higher milk fat percentage in both Holstein and other indigenous dairy cattle breeds, whereas those with the alanine (A) allele show higher milk protein content and total milk yield [151–154].”
Frequency of genetic variations associated with milk yield and composition in seven Zebu breeds
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- Authors
- Gothwal, A., Magotra, A., Bangar, Y. C., Malik, B. S., Yadav, A. S., & Garg, A. R.
- Affiliations
- Lala Lajpat Rai University of Veterinary and Animal Sciences (IN)
- Corresponding author
- Magotra, Ankit
- Venue
- Animal Biotechnology
- Publisher
- Informa UK Limited
- Published
- 2022-08-12
- Volume
- 34
- Issue
- 7
- Pages
- 2608-2616
- ISSN
- 1049-5398 (ISSN-L)
- 1532-2378
- OpenAlex
- W4290960381
- Cited by
- 8
- References
- 42
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 3 · 2023: 1
- FWCI
- 0.90
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Animal Biotechnology (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic and phenotypic traits in livestock
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Genetic Mapping and Diversity in Plants and Animals
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Animal Genetics and Reproduction
- Keywords
- DGAT1 gene, single nucleotide polymorphisms, dairy cattle, Sahiwal cattle, milk yield, reproductive traits, genetic diversity, Hardy-Weinberg equilibrium, candidate markers
- MeSH terms
- Animals, Cattle, Female, Genotype, Lactation, Milk, Mutation, Polymorphism, Genetic, Reproduction, Diacylglycerol O-Acyltransferase
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