CoChat
Sign Up
Cited by 117Caveolin-1 and cellular processesBarrier Structure and Function StudiesCholesterol and Lipid Metabolism

The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.

Physiological Pathway for Low-Density Lipoproteins across the Blood-Brain Barrier: Transcytosis through Brain Capillary Endothelial Cells In Vitro

Open access
Candela, Pietra, Gosselet, Fabien, Miller, Florence, Buee-Scherrer, Valérie, Torpier, Gérard, Cecchelli, Roméo, Fenart, Laurence·2008·Endothelium
DOI 10.1080/10623320802487759
Open this paper in CoChat — free account

Abstract

Although an immense knowledge has accumulated concerning regulation of cholesterol homeostasis in the body, this does not include the brain, where details are just emerging. Using an in vitro blood-brain barrier model, the authors have demonstrated that low-density lipoprotein (LDL) underwent transcytosis through the endothelial cells (ECs) by a receptor-mediated process, bypassing the lysosomal compartment. Moreover, caveolae might be involved in these blood-borne molecule transports from the blood to the brain. Although several ligands are known to be internalized through cell surface caveolae, the subsequent intracellular pathways have remained elusive. By cell fractionation experiment and Western blot, the authors have demonstrated that the LDL receptor is located in the caveolae membrane fraction. Then, LDLs internalized were detected by electron microscopy in multivesicular bodies. The authors identified in brain capillary ECs a novel endosomal compartment, mildly acidic, positive for marker Lamp-1 but devoid of any degradative capability. From the point of view of pH, cellular location, and caveolae-derived formation, the multivesicular organelles described here can be related to the caveosome structure. These results could provide clues to physiological functions of caveolae-caveosome transcellular pathway in brain capillary ECs and may help in the rational design of more effective therapeutic drugs to the brain.

Overview

Integrity signals

Registry

Crossref · 10.1080/10623320802487759

Cites

52 of 52 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Verification

Claims the paper makes

We couldn’t retrieve this paper’s full text, so its claims and self-check weren’t run. The registry and citation checks still apply.

Citations

Cites

Flagged first, then by citations

Show all 52 references

Research works printed but not linked: 3

  1. Booher J., 1972, Neurobiology, 2, 97
  2. Kirsch C., 2003, Pharmacopsychiatry, 36, 113
  3. Simionescu M., 1991, Cell Biology Review, 25, 5

Cited by

Sorted by citations

Items 101–117 of 117

Related

See all

Metadata

Authors
Candela, P., Gosselet, F., Miller, F., Buee-Scherrer, V., Torpier, G., Cecchelli, R., & Fenart, L.
Affiliations
  • Laboratoire Jean Perrin (FR)
  • Inserm (FR)
ORCID
Venue
Endothelium
Publisher
Informa UK Limited
Published
2008-01
Volume
15
Issue
5-6
Pages
254-264
ISSN
  • 1026-793X (ISSN-L)
  • 1062-3329
  • 1029-2373
DOI
10.1080/10623320802487759
OpenAlex
W2147613942
Cited by
117
References
52
Citations by year
2025: 6 · 2024: 6 · 2023: 5 · 2022: 5 · 2021: 6 · 2020: 11 · 2019: 4 · 2018: 7 · 2017: 6 · 2016: 5 · 2015: 10 · 2014: 12 · 2013: 8 · 2012: 8
FWCI
1.79
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Endothelium (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, cc-by)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Caveolin-1 and cellular processes
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Cholesterol and Lipid Metabolism
Keywords
low-density lipoprotein, blood-brain barrier, transcytosis, brain microvascular endothelial cells, caveolae, LDL receptor, multivesicular bodies, LAMP1, cholesterol homeostasis, receptor-mediated transport, endosomes
MeSH terms
Animals, Astrocytes, Blood-Brain Barrier, Brain, Capillaries, Cattle, Cell Membrane, Cells, Cultured, Cerebral Arteries, Cholesterol, Endocytosis, Endothelium, Vascular, Lipoproteins, LDL, Receptors, LDL, Endosomes, Coculture Techniques, Protein Transport, Caveolae, Transport Vesicles, Endothelial Cells, Microscopy, Electron, Transmission, Rats, Lysosomal Membrane Proteins, Lysosomal-Associated Membrane Protein 1

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review