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Cited by 117Caveolin-1 and cellular processesBarrier Structure and Function StudiesCholesterol and Lipid Metabolism

The DOI matches its Crossref record; 52 of the 52 references it cites were found in a registry or an index.

Physiological Pathway for Low-Density Lipoproteins across the Blood-Brain Barrier: Transcytosis through Brain Capillary Endothelial Cells In Vitro

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Candela, Pietra, Gosselet, Fabien, Miller, Florence, Buee-Scherrer, Valérie, Torpier, Gérard, Cecchelli, Roméo, Fenart, Laurence·2008·Endothelium
DOI 10.1080/10623320802487759
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Abstract

Although an immense knowledge has accumulated concerning regulation of cholesterol homeostasis in the body, this does not include the brain, where details are just emerging. Using an in vitro blood-brain barrier model, the authors have demonstrated that low-density lipoprotein (LDL) underwent transcytosis through the endothelial cells (ECs) by a receptor-mediated process, bypassing the lysosomal compartment. Moreover, caveolae might be involved in these blood-borne molecule transports from the blood to the brain. Although several ligands are known to be internalized through cell surface caveolae, the subsequent intracellular pathways have remained elusive. By cell fractionation experiment and Western blot, the authors have demonstrated that the LDL receptor is located in the caveolae membrane fraction. Then, LDLs internalized were detected by electron microscopy in multivesicular bodies. The authors identified in brain capillary ECs a novel endosomal compartment, mildly acidic, positive for marker Lamp-1 but devoid of any degradative capability. From the point of view of pH, cellular location, and caveolae-derived formation, the multivesicular organelles described here can be related to the caveosome structure. These results could provide clues to physiological functions of caveolae-caveosome transcellular pathway in brain capillary ECs and may help in the rational design of more effective therapeutic drugs to the brain.

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Crossref · 10.1080/10623320802487759

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Research works printed but not linked: 3

  1. Booher J., 1972, Neurobiology, 2, 97
  2. Kirsch C., 2003, Pharmacopsychiatry, 36, 113
  3. Simionescu M., 1991, Cell Biology Review, 25, 5

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Metadata

Authors
Candela, P., Gosselet, F., Miller, F., Buee-Scherrer, V., Torpier, G., Cecchelli, R., & Fenart, L.
Affiliations
  • Laboratoire Jean Perrin (FR)
  • Inserm (FR)
ORCID
Venue
Endothelium
Publisher
Informa UK Limited
Published
2008-01
Volume
15
Issue
5-6
Pages
254-264
ISSN
  • 1026-793X (ISSN-L)
  • 1062-3329
  • 1029-2373
DOI
10.1080/10623320802487759
OpenAlex
W2147613942
Cited by
117
References
52
Citations by year
2025: 6 · 2024: 6 · 2023: 5 · 2022: 5 · 2021: 6 · 2020: 11 · 2019: 4 · 2018: 7 · 2017: 6 · 2016: 5 · 2015: 10 · 2014: 12 · 2013: 8 · 2012: 8
FWCI
1.79
Type
journal article
Language
en
Open access
Licence
cc-by
Locations
  • Endothelium (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, cc-by)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Caveolin-1 and cellular processes
  • Life Sciences › Neuroscience › Neurology › Barrier Structure and Function Studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Cholesterol and Lipid Metabolism
Keywords
low-density lipoprotein, blood-brain barrier, transcytosis, brain microvascular endothelial cells, caveolae, LDL receptor, multivesicular bodies, LAMP1, cholesterol homeostasis, receptor-mediated transport, endosomes
MeSH terms
Animals, Astrocytes, Blood-Brain Barrier, Brain, Capillaries, Cattle, Cell Membrane, Cells, Cultured, Cerebral Arteries, Cholesterol, Endocytosis, Endothelium, Vascular, Lipoproteins, LDL, Receptors, LDL, Endosomes, Coculture Techniques, Protein Transport, Caveolae, Transport Vesicles, Endothelial Cells, Microscopy, Electron, Transmission, Rats, Lysosomal Membrane Proteins, Lysosomal-Associated Membrane Protein 1

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