Sequestration and Ultrasound-Induced Release of Doxorubicin from Stabilized Pluronic P105 Micelles
Open accessAbstract
Controlled drug delivery from micelles requires that the micelles remain stable when diluted below their critical micelle concentration, such as upon injection into blood. A cross-linked, interpenetrating network of N,N-diethylacrylamide (NNDEA) was polymerized in the core of Pluronic P105 micelles to stabilize temporarily the micelles at concentrations below the critical micellar concentration of free P105. The stabilized Pluronic micelles (called Plurogels) were able to sequester the drug doxorubicin (Dox) and protect HL-60 cells from the drug at concentrations where non-stabilized Pluronic provided no protection. The protection lasted approximately 12 hr, which is similar to the half-life of the particles. Application of low-frequency ultrasound resulted in a synergistic killing effect with Dox and low concentrations of either Pluronic P105 or stabilized Plurogels, most probably due to release of Dox and permeabilization of the cell membrane.
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Pruitt and Pitt developed an approach in which DOX stabilized with PEG-phospholipid was encapsulated in ultrasound-sensitive nanomicelles.
“Another approach has been developed by Pruitt and Pitt in which DOX stabilized with PEG-phospholipid was encapsulated in ultrasound-sensitive nanomicelles [207].”
From Recent Advances in Nanomicelles Delivery Systems. (2020) · cites this paper as [207]
The Abstract reports that stabilized Pluronic P105 micelles sequester doxorubicin and respond to low-frequency ultrasound. But it identifies the stabilizer as a cross-linked N,N-diethylacrylamide network, not PEG-phospholipid; the Materials section likewise lists the former components. (LLM, conf=0.90)
Experimental — accuracy not yet measuredIn vivo studies revealed that the release of DOX was significantly increased upon treatment with ultrasound that, as a result, retarded tumor growth higher than without treatment.
“In vivo studies revealed that the release of DOX was significantly increased upon treatment with ultrasound that, as a result, retarded tumor growth higher than without treatment.”
From Recent Advances in Nanomicelles Delivery Systems. (2020) · cites this paper as [207]
The Sonicated Cell Experiments section reports that ultrasound releases some DOX from micelles and increases DOX-associated cell death. But it explicitly says the results presented are from one cell line in vitro; it gives no comparison of tumor growth with and without ultrasound in vivo. The rat colon cancer results are described only as encouraging and to be reported elsewhere (page unavailable in our copy). (LLM, conf=0.90)
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Cites
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Flagged first, then by citations
Research works printed but not linked: 1
- Pruitt, J. 2001. Stabilization of Pluronic P-105 for targeted nanoparticle drug delivery. Dissertation, Chemical Engineering, Brigham Young University, Provo, Utah.
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Physical stimuli-responsive polymeric micelles for anti-cancer drug delivery
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“Another approach has been developed by Pruitt and Pitt in which DOX stabilized with PEG-phospholipid was encapsulated in ultrasound-sensitive nanomicelles [207].”
MethodUltrasonically triggered drug delivery: Breaking the barrier
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Self-citation
- Authors
- Pruitt, J. D., & Pitt, W. G.
- Affiliations
- Brigham Young University (US)
- ORCID
- Pitt, William G.
- Venue
- Drug Delivery
- Publisher
- Informa UK Limited
- Published
- 2002-01
- Volume
- 9
- Issue
- 4
- Pages
- 253-258
- ISSN
- 1071-7544 (ISSN-L)
- 1521-0464
- OpenAlex
- W1969042044
- Cited by
- 63
- References
- 17
- Citations by year
- 2022: 3 · 2021: 3 · 2020: 4 · 2019: 2 · 2018: 2 · 2017: 1 · 2016: 4 · 2015: 1 · 2014: 3 · 2013: 2 · 2012: 3
- FWCI
- 1.50
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Drug Delivery (publishedVersion)
- PubMed (publishedVersion)
- ScholarsArchive (Brigham Young University) (submittedVersion)
- Topics
- Physical Sciences › Materials Science › Biomaterials › Nanoparticle-Based Drug Delivery
- Physical Sciences › Engineering › Biomedical Engineering › Ultrasound and Hyperthermia Applications
- Physical Sciences › Chemistry › Organic Chemistry › Surfactants and Colloidal Systems
- Keywords
- doxorubicin, Pluronic P105, micelle stabilization, N,N-diethylacrylamide, crosslinked polymer networks, low-frequency ultrasound, HL-60 cells, drug sequestration, controlled drug delivery, cell membrane permeabilization, synergistic cytotoxicity
- MeSH terms
- Doxorubicin, Excipients, Humans, Micelles, Ultrasonics, HL-60 Cells, Poloxamer
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