CoChat
Sign Up
Cited by 111Garlic and Onion StudiesAlcohol Consumption and Health EffectsLiver physiology and pathology

The DOI matches its Crossref record; 34 of the 35 references it cites were found in a registry or an index.

Effects of garlic polysaccharide on alcoholic liver fibrosis and intestinal microflora in mice

Open access
Wang, Yuchuan, Guan, Min, Zhao, Xin, Li, Xinli·2018·Pharmaceutical Biology
DOI 10.1080/13880209.2018.1479868PMC PMC6130653
Open this paper in CoChat — free accountOpen PDF

Abstract

CONTEXT: Alcoholic liver fibrosis (ALF) is treatable and reversible consequence of liver disease. Intestinal microflora plays an important role in the progression of liver disease. Garlic (Allium sativum L. [Amaryllidaceae]) has been consumed as a traditional medicine to treat liver injury. OBJECTIVE: To investigate the effects of garlic polysaccharide (GP) on ALF and intestinal microflora in mice. MATERIALS AND METHODS: KM mice were orally administered with alcohol (56%, 6 mL/kg) for 30 d to establish ALF model, and divided into four groups together with control group (water only). Hugan tablet (60 mg/kg) or GP (250 and 150 mg/kg) were given 5 h after each dose of alcohol. Biochemical markers in serum and liver homogenate were determined with kits. Alteration of intestinal microflora, and protein expressions of TGF-β1, TNF-α and decorin were detected. RESULTS: In GP-H group, ALT and AST decreased to 18.85 ± 4.71 U/L and 40.84 ± 7.89 U/L. MDA, TC, TG and LDL-C decreased to 2.32 ± 0.86 mmol/mg, 0.21 ± 0.12 mmol/L, 0.96 ± 0.31 mmol/L and 0.084 ± 0.027 mmol/L. SOD, GSH-Px and GSH increased to 118.32 ± 16.32 U/mg, 523.72 ± 64.20 U/mg and 0.56 ± 0.05 mg/g. Ratios of TGF-β1 and TNF-α decreased to 0.608 ± 0.170 and 1.057 ± 0.058, decorin increased to 2.182 ± 0.129. Lachnospiraceae and Lactobacillus increased, Facklamia and Firmicutes decreased with GP pretreatment. DISCUSSION AND CONCLUSIONS: Intestinal microflora provides novel insight into the mechanisms of GP that may be used to treat ALF and intestinal microflora dysbiosis.

Overview

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Integrity signals

Registry

Crossref · 10.1080/13880209.2018.1479868

Cites

34 of 34 references verified0 citation errors · 0 not checkable

Statistics

Figures

Figure 1. Elution curve of crude polysaccharide on DEAE-52 cellulose chromatography (A) and HPLC profile of GP (B).
Figure 1. Elution curve of crude polysaccharide on DEAE-52 cellulose chromatography (A) and HPLC profile of GP (B).CC BY · PubMed Central
Figure 2. Effects of GP on the relative liver weight (%) (A), the levels of ALT and AST in serum (B), the activity of SOD in liver homogenate (C), the level of MDA in liver homogenate (D), the level of GSH in liver homogenate (E), the activity of GSH-Px in liver homogenate (F), the levels of TC, TG, HDL and LDL in liver homogenate (G), and H&E staining for histopathological examination (100×) (H). GP-H: GP-treated (250 mg/kg) group; GP-L: GP-treated (150 mg/kg) group. Values are expressed as mean ± SD (n = 10). *p < 0.05 and **p < 0.01 versus negative group; ##p < 0.01 versus normal control group. HC: hepatocytes; CV: central vein; S: sinusoid; DS: dilated sinusoid; IN: cellular inflammation; BH: ballooned hepatocytes.
Figure 2. Effects of GP on the relative liver weight (%) (A), the levels of ALT and AST in serum (B), the activity of SOD in liver homogenate (C), the level of MDA in liver homogenate (D), the level of GSH in liver homogenate (E), the activity of GSH-Px in liver homogenate (F), the levels of TC, TG, HDL and LDL in liver homogenate (G), and H&E staining for histopathological examination (100×) (H). GP-H: GP-treated (250 mg/kg) group; GP-L: GP-treated (150 mg/kg) group. Values are expressed as mean ± SD (n = 10). *p < 0.05 and **p < 0.01 versus negative group; ##p < 0.01 versus normal control group. HC: hepatocytes; CV: central vein; S: sinusoid; DS: dilated sinusoid; IN: cellular inflammation; BH: ballooned hepatocytes.CC BY · PubMed Central
Figure 3. Effects of GP on protein expression of TGF-β1, TNF-α and decorin in livers. GP-H: GP-treated (250 mg/kg) group; GP-L: GP-treated (150 mg/kg) group. Values are expressed as mean ± SD (n = 10). *p < 0.05 and **p < 0.01 versus negative group; ##p < 0.01 versus normal control group.
Figure 3. Effects of GP on protein expression of TGF-β1, TNF-α and decorin in livers. GP-H: GP-treated (250 mg/kg) group; GP-L: GP-treated (150 mg/kg) group. Values are expressed as mean ± SD (n = 10). *p < 0.05 and **p < 0.01 versus negative group; ##p < 0.01 versus normal control group.CC BY · PubMed Central
Figure 4. Representative DGGE profiles (A) and UPGMA dendrograms (B) of intestinal microflora. GP-H: GP-treated (250 mg/kg) group; GP-L: GP-treated (150 mg/kg) group.
Figure 4. Representative DGGE profiles (A) and UPGMA dendrograms (B) of intestinal microflora. GP-H: GP-treated (250 mg/kg) group; GP-L: GP-treated (150 mg/kg) group.CC BY · PubMed Central

Verification

Claims the paper makes

The claims and statistics of this paper haven’t been checked yet.

Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.

A few free checks a day · papers already verified open instantly · results are public

Claims other papers make about this one

Each is checked against this paper’s own text: does it say what the citing paper says it does?

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The self-check needs the paper’s full text.

Citations

Cites

Show all 35 references

Research works printed but not linked: 2

  1. Bode JC, 1984, Hepato-gastroenterology, 31, 30
  2. Staub AM, 1965, Method Carbohydr Chem, 5, 5

Cited by

Sorted by citations

Items 101–111 of 111

Related

See all

Metadata

Authors
Wang, Y., Guan, M., Zhao, X., & Li, X.
Affiliations
Dalian Medical University (CN)
Corresponding author
Li, Xinli
ORCID
Venue
Pharmaceutical Biology
Publisher
Informa UK Limited
Published
2018-01-01
Volume
56
Issue
1
Pages
325-332
ISSN
  • 0925-1618 (ISSN-L)
  • 1388-0209
  • 1744-5116
DOI
10.1080/13880209.2018.1479868
PMC
PMC6130653
OpenAlex
W2810636402
Cited by
110
References
35
Citations by year
2026: 9 · 2025: 6 · 2024: 22 · 2023: 12 · 2022: 24 · 2021: 17 · 2020: 9 · 2019: 10
FWCI
7.96
Type
journal article
Language
en
Open access
Licence
Locations
  • Pharmaceutical Biology (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Funders
Foundation of Liaoning Educational Committee of China (L2016004)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Garlic and Onion Studies
  • Health Sciences › Medicine › Pathology and Forensic Medicine › Alcohol Consumption and Health Effects
  • Health Sciences › Medicine › Hepatology › Liver physiology and pathology
Keywords
garlic polysaccharide, alcoholic liver fibrosis, gut microbiota, gut dysbiosis, alcohol-induced liver injury, TGF-β1, TNF-α, decorin, antioxidant enzymes, Lachnospiraceae, Lactobacillus, Firmicutes, garlic
MeSH terms
Gastrointestinal Microbiome, Animals, Garlic, Liver Diseases, Alcoholic, Male, Plant Extracts, Polysaccharides, Treatment Outcome, Mice

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review