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Cited by 1,038Gut microbiota and healthLiver Disease and TransplantationMicrobial Community Ecology and Physiology

Characterization of fecal microbial communities in patients with liver cirrhosis

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Chen, Yanfei, Yang, Fengling, Lu, Haifeng, Wang, Baohong, Chen, Yunbo, Lei, Dajiang, Wang, Yuezhu, Zhu, Baoli +1 more·2011·Hepatology
DOI 10.1002/hep.24423
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Abstract

UNLABELLED: Liver cirrhosis is the pathologic end stage of chronic liver disease. Increasing evidence suggests that gut flora is implicated in the pathogenesis of liver cirrhosis complications. The aim of this study was to characterize the fecal microbial community in patients with liver cirrhosis in comparison with healthy individuals. We recruited 36 patients with liver cirrhosis and 24 healthy controls. The fecal microbial communities was analyzed by way of 454 pyrosequencing of the 16S ribosomal RNA V3 region followed by real-time quantitative polymerase chain reaction. Community-wide changes of fecal microbiota in liver cirrhosis were observed compared with healthy controls. The proportion of phylum Bacteroidetes was significantly reduced (P=0.008), whereas Proteobacteria and Fusobacteria were highly enriched in the cirrhosis group (P=0.001 and 0.002, respectively). Enterobacteriaceae (P=0.001), Veillonellaceae (P=0.046), and Streptococcaceae (P=0.001) were prevalent in patients with cirrhosis at the family level. A positive correlation was observed between Child-Turcotte-Pugh (CTP) score and Streptococcaceae (R=0.386, P=0.02). Lachnospiraceae decreased significantly in patients with cirrhosis (P=0.004) and correlated negatively with CTP score (R=-0.49, P=0.002). Using partial least square discriminate analysis, we identified 149 operational taxonomic units (OTUs) as key phylotypes that responded to cirrhosis, most of which were Lachnospiraceae (65 OTUs), Streptococcaceae (23 OTUs), and Veillonellaceae (21 OTUs). CONCLUSION: Fecal microbial communities are distinct in patients with cirrhosis compared with healthy individuals. The prevalence of potentially pathogenic bacteria, such as Enterobacteriaceae and Streptococcaceae, with the reduction of beneficial populations such as Lachnospiraceae in patients with cirrhosis may affect prognosis.

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  • Patients with cirrhosis have reduced abundance of beneficial gut bacteria such as Lachnospiraceae.

    “At the same time, there is decreased abundance of beneficial bacteria in the gut, such as Lachnospiraceae33.”

    From The gut microbiome and liver cancer: mechanisms and clinical translation (2017) · cites this paper as [33]

  • Cirrhosis stage correlates positively with Enterobacteriaceae and negatively with Lachnospiraceae.

    “These changes seem to develop progressively, as cirrhosis stage is positively correlated with Enterobacteriaceae and negatively correlated with Lachnospiraceae33,34.”

    From The gut microbiome and liver cancer: mechanisms and clinical translation (2017) · cites this paper as [33]

  • Patients with cirrhosis have increased Enterobacteriaceae and oral-type taxa such as Veillonellaceae and Streptococcaceae, consistent with microorganisms moving from the mouth to the gut.

    “Studies in the past few years have demonstrated profound alterations of the intestinal microbiota in patients with cirrhosis, showing increased Enterobacteriacea as well as strains that are typically found in the oral microbiota, such as Veillonellaceae and Streptococcaceae, consistent with an invasion of microorganisms from the mouth to the gut in liver cirrhosis32,33.”

    From The gut microbiome and liver cancer: mechanisms and clinical translation (2017) · cites this paper as [33]

  • Chen et al. found a similar Chao index in cirrhotic patients and a non-significant Shannon index when compared to healthy controls.

    “Chen et al.51 found a similar Chao index in cirrhotic patients and a non-significant Shannon index when compared to healthy controls.”

    From Cholestasis induced by bile duct ligation promotes changes in the intestinal microbiome in mice (2019) · cites this paper as [51]

  • The composition of the microbiome was altered in all these patients regardless of the lack of statistically significant changes in diversity.

    “the composition of the microbiome was altered in all these patients regardless of the lack of statistically significant changes in diversity”

    From Cholestasis induced by bile duct ligation promotes changes in the intestinal microbiome in mice (2019) · cites this paper as [51]

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Cited by

Sorted by citations

Authors
Chen, Y., Yang, F., Lu, H., Wang, B., Chen, Y., Lei, D., Wang, Y., Zhu, B., & Li, L.
Affiliations
  • First Affiliated Hospital Zhejiang University (CN)
  • Chinese National Human Genome Center at Shanghai (CN)
  • Chinese Academy of Sciences (CN)
Corresponding author
  • Zhu, Baoli
  • Li, Lanjuan
ORCID
Venue
Hepatology
Publisher
Ovid Technologies (Wolters Kluwer Health)
Published
2011-08
Volume
54
Issue
2
Pages
562-572
ISSN
0270-9139 (ISSN-L)
DOI
10.1002/hep.24423
OpenAlex
W2051567523
Cited by
1,038
References
4
Citations by year
2026: 38 · 2025: 66 · 2024: 77 · 2023: 119 · 2022: 103 · 2021: 101 · 2020: 108 · 2019: 85 · 2018: 96 · 2017: 68 · 2016: 40 · 2015: 55 · 2014: 33 · 2013: 27 · 2012: 17
FWCI
10.58
Type
journal article
Language
en
Open access
Licence
doi.wiley.com/10.1002/tdm_license_1.1 (tdm, 1492 d)
Locations
  • Hepatology (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Gut microbiota and health
  • Health Sciences › Medicine › Hepatology › Liver Disease and Transplantation
  • Physical Sciences › Environmental Science › Ecology › Microbial Community Ecology and Physiology
Keywords
liver cirrhosis, gut microbiota, Bacteroidetes, Lachnospiraceae, Streptococcaceae, Veillonellaceae, Enterobacteriaceae, Fusobacteria, Proteobacteria, Child-Pugh score, gut dysbiosis, cirrhosis complications
MeSH terms
Bacteria, Feces, Female, Humans, Liver Cirrhosis, Male, Middle Aged

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