Phytochemical, cytotoxic and chemotaxonomic study on Ajuga forrestii Diels (Labiatae)
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A phytochemical investigation of Ajuga forrestii Diels led to the isolation of 14 compounds, including eight neo-clerodane diterpenes (1-8), two phytoecdysteroids (9, 11), one stigmastane sterol (10) and three iridoid glycosides (12-14). The structures of these compounds were identified by spectroscopic methods and a comparison of their data with those reported in the literature. This is the first report of compounds 1-14 from A. forrestii. The cytotoxic activities of the aqueous extract of A. forrestii and several compounds have been studied and the chemotaxonomic significance of isolated compounds has also been summarised.
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Ajugacetalsterone A showed significant cytotoxic/antiproliferative activity against A-549, HepG2, MCF-7, and HeLa cells with IC50 values of 26.5±1.3, 33.3±1.4, 25.4±1.8, and 28.1±2.9 μmol/L, respectively.
“Evaluated cytotoxicity against human lung cancer cell A-549, human hepatoma cell HepG2, human breast cancer cell MCF-7 and proliferating epidermal carcinoma cell HeLa using MTT method and significant activity have been observed.”
From The Phytochemical, Biological, and Medicinal Attributes of Phytoecdysteroids: An Updated Review (2021) · cites this paper as [170]
An aqueous extract of A. forrestii containing iridoid compounds exhibited potential cytotoxicity with IC50 values between 10 and 19 μmol/L, while selected iridoid glycosides exhibited more significant cytotoxicities with IC50 values between 7 and 14 μmol/L.
“An aqueous extract of A. forrestii containing iridoid compounds exhibited potential cytotoxicity with IC50 values ranging between 10 and 19 μmol/L, while selected iridoid glycosides exhibited more significant cytotoxicities with IC50 values ranging between 7 and 14 μmol/L170.”
From The Phytochemical, Biological, and Medicinal Attributes of Phytoecdysteroids: An Updated Review (2021) · cites this paper as [170]
Two phytoecdysteroids extracted from Ajuga forrestii showed no significant inhibitory activities against A-549, HepG2, MCF-7, and HeLa cells.
“Two PEs extracted from Ajuga forrestii and evaluated for their cytotoxic effects against the A-549 human lung cancer cell, HepG2 human hepatoma cell, MCF-7 human breast cancer cell, and HeLa human cervical carcinoma cell showed no significant inhibitory activities.”
From The Phytochemical, Biological, and Medicinal Attributes of Phytoecdysteroids: An Updated Review (2021) · cites this paper as [170]
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- Authors
- Chen, T., Diao, Q.-Y., Yu, H.-Z., Jiao, C.-L., & Ruan, J.
- Affiliations
- Yantai Center for Food and Drug Control and Test, Yantai, China
- Longkou Market Supervision Administration, Yantai, China
- Corresponding author
- Ruan, Jian
- Venue
- Natural Product Research
- Publisher
- Informa UK Limited
- Published
- 2017-09-06
- Volume
- 32
- Issue
- 8
- Pages
- 977-981
- ISSN
- 1478-6419 (ISSN-L)
- 1478-6427
- OpenAlex
- W2752978181
- Cited by
- 16
- References
- 23
- Citations by year
- 2026: 1 · 2025: 1 · 2024: 3 · 2023: 2 · 2022: 2 · 2021: 1 · 2020: 3 · 2019: 2 · 2018: 1
- FWCI
- 0.67
- Type
- journal article
- Language
- en
- Open access
- green
- figshare.com
- Licence
- cc-by
- Locations
- Natural Product Research (publishedVersion)
- PubMed (publishedVersion)
- Figshare (submittedVersion, cc-by)
- Figshare (cc-by)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bioactive Natural Diterpenoids Research
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Phytochemistry and Biological Activities
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Biological Activity of Diterpenoids and Biflavonoids
- Keywords
- neo-clerodane diterpenoids, phytoecdysteroids, iridoid glycosides, cytotoxicity, chemotaxonomy, Lamiaceae, natural product isolation, structure elucidation
- MeSH terms
- Antineoplastic Agents, Phytogenic, Drug Screening Assays, Antitumor, Humans, Magnetic Resonance Spectroscopy, Plant Extracts, Molecular Structure, Spectrometry, Mass, Electrospray Ionization, Ajuga, Cell Line, Tumor, Diterpenes, Clerodane, Iridoid Glycosides, Phytochemicals
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