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Comparison of DNA methylation measured by Illumina 450K and EPIC BeadChips in blood of newborns and 14-year-old children

Solomon, Olivia, MacIsaac, Julie, Quach, Hong, Tindula, Gwen, Kobor, Michael S., Huen, Karen, Meaney, Michael J., Eskenazi, Brenda, Barcellos, Lisa F., Holland, Nina·2018·Epigenetics

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Crossref · 10.1080/15592294.2018.1497386

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Figures

Figure 1. β values for all overlapping probes on 450K vs. beta values on EPIC for a newborn sample.
Figure 1. β values for all overlapping probes on 450K vs. beta values on EPIC for a newborn sample.CC BY-NC-ND · PubMed Central
Figure 2. Distribution of correlation coefficients for individual CpG sites, separated by probe type. Type II probes have a greater density of CpG sites with higher correlation coefficients.
Figure 2. Distribution of correlation coefficients for individual CpG sites, separated by probe type. Type II probes have a greater density of CpG sites with higher correlation coefficients.CC BY-NC-ND · PubMed Central
Figure 3. The correlation of β values between 450K and EPIC relative to the variance of methylation at each site (top). Sites in the lowest quartile of correlation had low variance and were mostly completely methylated or completely unmethylated (bottom left). Sites in the highest quartile of correlation had high variance of methylation (bottom right).
Figure 3. The correlation of β values between 450K and EPIC relative to the variance of methylation at each site (top). Sites in the lowest quartile of correlation had low variance and were mostly completely methylated or completely unmethylated (bottom left). Sites in the highest quartile of correlation had high variance of methylation (bottom right).CC BY-NC-ND · PubMed Central
Figure 4. The absolute difference in β value at each CpG site between 450K and EPIC for newborns is plotted on the y-axis. Sites which also showed large differences >0.25 in the 14-year data set are highlighted in green and counts of sites exceeding thresholds of 0.1, 0.25, and 0.5 are shown on the right side of the plot.
Figure 4. The absolute difference in β value at each CpG site between 450K and EPIC for newborns is plotted on the y-axis. Sites which also showed large differences >0.25 in the 14-year data set are highlighted in green and counts of sites exceeding thresholds of 0.1, 0.25, and 0.5 are shown on the right side of the plot.CC BY-NC-ND · PubMed Central
Figure 5. Regression β values for all overlapping probes on 450K vs. regression beta values on EPIC analyses of differentially methylation positions by sex in newborns.
Figure 5. Regression β values for all overlapping probes on 450K vs. regression beta values on EPIC analyses of differentially methylation positions by sex in newborns.CC BY-NC-ND · PubMed Central
Figure 6. Manhattan plot showing differentially methylation positions by sex in newborns on 450K in autosomes. Sites highlighted in green are the 999 of the top 1,000 autosomal 450K hits, which were significant (FDR q <0.05) in EPIC analyses.
Figure 6. Manhattan plot showing differentially methylation positions by sex in newborns on 450K in autosomes. Sites highlighted in green are the 999 of the top 1,000 autosomal 450K hits, which were significant (FDR q <0.05) in EPIC analyses.CC BY-NC-ND · PubMed Central

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Citations

Cites

Research works printed but not linked: 17

  1. 10.1186/s13059-016-1066-1 · DOI · Paper page
  2. 10.1093/ije/dyx190 · DOI
  3. 10.1186/s13148-017-0333-7 · DOI
  4. 10.2217/epi-2017-0078 · DOI · Paper page
  5. 10.1038/pr.2017.112 · DOI
  6. 10.1093/bioinformatics/btu049 · DOI
  7. 10.1289/EHP595 · DOI
  8. 10.3109/713610244 · DOI
  9. 10.1289/ehp.8540 · DOI
  10. 10.4161/epi.26037 · DOI
  11. 10.1093/nar/gkw967 · DOI
  12. 10.1186/1756-8935-6-4 · DOI
  13. 10.4161/epi.23470 · DOI
  14. 10.1080/15592294.2016.1161875 · DOI
  15. 10.1093/nar/gkw1138 · DOI
  16. 10.2202/1544-6115.1027 · DOI · Paper page
  17. R Core Team, 2017, R: A language and environment for statistical computing

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Metadata

Authors
Solomon, O., MacIsaac, J., Quach, H., Tindula, G., Kobor, M. S., Huen, K., Meaney, M. J., Eskenazi, B., Barcellos, L. F., & Holland, N.
Venue
Epigenetics
Published
2018-06-03
Volume
13
Issue
6
Pages
655-664
ISSN
  • 1559-2294
  • 1559-2308
Cited by
94
References
4
Language
en
Funders
  • California Environmental Protection Agency (RD83171001, RD83273401)
  • National Institute of Environmental Health Sciences (R01ES021369, R24ES028529, P01ES009605, R01ES012503, F31 ES027751)
  • National Institutes of Health (UG3OD023356)

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