Cited by 57Infection Control and VentilationSARS-CoV-2 detection and testingDental Research and COVID-19

Size distribution of exhaled aerosol particles containing SARS-CoV-2 RNA

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Alsved, Malin, Nygren, David, Thuresson, Sara, Fraenkel, Carl-Johan, Medstrand, Patrik, Löndahl, Jakob·2023·Infectious Diseases

Abstract

BACKGROUND: SARS-CoV-2 in exhaled aerosols is considered an important contributor to the spread of COVID-19. However, characterizing the size distribution of virus-containing aerosol particles has been challenging as high concentrations of SARS-CoV-2 in exhaled air is mainly present close to symptom onset. We present a case study of a person with COVID-19 who was able to participate in extensive measurements of exhaled aerosols already on the day of symptom onset and then for the following three days. METHODS: Aerosol collection was performed using an eight-stage impactor while the subject was breathing, talking and singing, for 30 min each, once every day. In addition, nasopharyngeal samples, saliva samples, room air samples and information on symptom manifestations were collected every day. Samples were analyzed by RT-qPCR for detection of SARS-CoV-2 RNA. RESULTS: SARS-CoV-2 RNA was detected in seven of the eight particle size fractions, from 0.34 to >8.1 µm, with the highest concentrations found in 0.94-2.8 µm particles. The concentration of SARS-CoV-2 RNA was highest on the day of symptom onset, and declined for each day thereafter. CONCLUSION: Our data showed that 90% of the exhaled SARS-CoV-2 RNA was found in aerosol particles <4.5 µm, indicating the importance of small particles for the transmission of COVID-19 close to symptom onset. These results are important for our understanding of airborne transmission, for developing accurate models and for selecting appropriate mitigation strategies.

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Crossref · 10.1080/23744235.2022.2140822

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Authors
Alsved, M., Nygren, D., Thuresson, S., Fraenkel, C.-J., Medstrand, P., & Löndahl, J.
Affiliations
Lund University (SE)
Corresponding author
Alsved, Malin
Venue
Infectious Diseases
Published
2022-11-04
Volume
55
Issue
2
Pages
158-163
ISSN
  • 0036-5548 (ISSN-L)
  • 2374-4235
  • 2374-4243
OpenAlex
W4308189368
Cited by
57
References
20
Citations by year
2026: 12 · 2025: 14 · 2024: 21 · 2023: 8 · 2022: 2
FWCI
7.31
Type
journal article
Language
en
Open access
Locations
  • Scandinavian Journal of Infectious Diseases (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • Lund University Publications (Lund University) (submittedVersion)
Funders
  • AFA insurance (180113, 200109)
  • Swedish Research Council FORMAS (2017-00383, 2020-01490)
  • Swedish Research Council FORTE (2017-00690)
  • Region Skåne
  • Swedish Research Council (2019-05235, 2020-02344)
  • SciLifeLab National COVID-19 Research Program
  • Knut and Alice Wallenberg Foundation
Topics
  • Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Infection Control and Ventilation
  • Health Sciences › Medicine › Infectious Diseases › SARS-CoV-2 detection and testing
  • Health Sciences › Dentistry › General Dentistry › Dental Research and COVID-19
Keywords
SARS-CoV-2, COVID-19 pandemic, exhaled aerosols, aerosol size distribution, airborne transmission, viral RNA, symptom onset, respiratory droplets
MeSH terms
COVID-19, SARS-CoV-2, Respiratory Aerosols and Droplets, Humans, RNA, Viral

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