The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Studies on the mechanism of membrane fusion: site-specific mutagenesis of the hemagglutinin of influenza virus.
Open accessAbstract
Oligonucleotide-directed mutagenesis of a cDNA encoding the hemagglutinin of influenza virus has been used to introduce single base changes into the sequence that codes for the conserved apolar "fusion peptide" at the amino-terminus of the HA2 subunit. The mutant sequences replaced the wild-type gene in SV40-HA recombinant virus vectors, and the altered HA proteins were expressed in simian cells. Three mutants have been constructed that introduce single, nonconservative amino acid changes in the fusion peptide, and three fusion phenotypes were observed: substitution of glutamic acid for the glycine residue at the amino-terminus of HA2 abolished all fusion activity; substitution of glutamic acid for the glycine residue at position 4 in HA2 raised the threshold pH and decreased the efficiency of fusion; and, finally, extension of the hydrophobic stretch by replacement of the glutamic acid at position 11 with glycine yielded a mutant protein that induced fusion of erythrocytes with cells with the same efficiency and pH profile as the wild-type protein. However, the ability of this mutant to induce polykaryon formation was greatly impaired. Nevertheless, all the mutant proteins underwent a pH-dependent conformational change and bound to liposomes. These results are discussed in terms of the mechanism of HA-induced membrane fusion.
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Cites
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Cited by
Receptor Binding and Membrane Fusion in Virus Entry: The Influenza Hemagglutinin
Skehel, J. J., & Wiley, D. C. (2000) · Annual Review of Biochemistry · doi:10.1146/annurev.biochem.69.1.531 · Cited by 2,706
Structure of influenza haemagglutinin at the pH of membrane fusion
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Wagner, E., Plank, C., Zatloukal, K., Cotten, M., & Birnstiel, M. L. (1992) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.89.17.7934 · Cited by 658
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Self-citationVIRUS-CELL AND CELL-CELL FUSION
Hernandez, L. D., Hoffman, L. R., Wolfsberg, T. G., & White, J. M. (1996) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.12.1.627 · Cited by 567
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Metadata
- Authors
- Gething, M. J., Doms, R. W., York, D., & White, J.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1986-01-01
- Volume
- 102
- Issue
- 1
- Pages
- 11-23
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2159031835
- Cited by
- 340
- References
- 49
- FWCI
- 18.00
- Type
- journal article
- Language
- en
- Open access
- green
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- Gething, M. J., Doms, R. W., York, D., & White, J. (1986). Studies on the mechanism of membrane fusion: site-specific mutagenesis of the hemagglutinin of influenza virus. The Journal of cell biology. https://doi.org/10.1083/jcb.102.1.11
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
