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A 17-kD centromere protein (CENP-A) copurifies with nucleosome core particles and with histones
Open accessAbstract
We have detected and begun to characterize a 17-kD centromere-specific protein, CENP-A (Earnshaw, W. C., and N. Rothfield, 1985, Chromosoma., 91:313-321). Sera from several humans with CREST scleroderma autoimmune disease (CREST: calcinosis, Raynaud's phenomenon, esophageal dsymotility, sclerodactyly, and telangiectasia) bind this protein in immunoblot assays of HeLa whole cell or nuclear extracts. We have affinity purified the anti-17-kD centromere protein (anti-CENP-A) specific antibodies from immunoblots of HeLa nuclear protein. The antibodies react with epitopes present on CENP-A derived from humans but apparently do not recognize specific epitopes in either rat or chicken nuclei. Only human nuclear protein is CENP-A positive by immunoblot. Furthermore, human cells show localization of anti-CENP-A antibody to centromeres by immunofluorescence microscopy, whereas rat cells do not. On extraction from the nucleus, CENP-A copurifies with core histones and with nucleosome core particles. We conclude that this centromere-specific protein is a histone-like component of chromatin. The data suggest that CENP-A functions as a centromere-specific core histone.
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Citations
Cited by
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Katayama, H., Brinkley, W. R., & Sen, S. (2003) · Cancer and Metastasis Reviews · doi:10.1023/a:1023789416385 · Cited by 398
Priming of Centromere for CENP-A Recruitment by Human hMis18α, hMis18β, and M18BP1
Fujita, Y., Hayashi, T., Kiyomitsu, T., Toyoda, Y., Kokubu, A., Obuse, C., & Yanagida, M. (2007) · Developmental Cell · doi:10.1016/j.devcel.2006.11.002 · Cited by 398
A two-step mechanism for epigenetic specification of centromere identity and function
Fachinetti, D., Diego Folco, H., Nechemia-Arbely, Y., Valente, L. P., Nguyen, K., Wong, A. J., Zhu, Q., Holland, A. J., Desai, A., Jansen, L. E. T., et al. (2013) · Nature Cell Biology · doi:10.1038/ncb2805 · Cited by 255
Metadata
- Authors
- Palmer, D., O'Day, K., Wener, M., Andrews, B., & Margolis, R.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1987-04-01
- Volume
- 104
- Issue
- 4
- Pages
- 805-815
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2121989845
- Cited by
- 430
- References
- 0
- FWCI
- 3.57
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: A 17-kD centromere protein (CENP-A) copurifies with nucleosome core particles and with histones. https://app.cochat.ai/papers/10.1083/jcb.104.4.805
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- Palmer, D., O'Day, K., Wener, M., Andrews, B., & Margolis, R. (1987). A 17-kD centromere protein (CENP-A) copurifies with nucleosome core particles and with histones. The Journal of cell biology. https://doi.org/10.1083/jcb.104.4.805
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