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Cited by 2,272Inflammatory mediators and NSAID effectsAngiogenesis and VEGF in CancerEicosanoids and Hypertension Pharmacology

Cyclooxygenase regulates angiogenesis induced by colon cancer cells

Open access
Tsujii, Masahiko, Kawano, Sunao, Tsuji, Shingo, Sawaoka, Hitoshi, Hori, Masatsugu, DuBois, Raymond N·1998·Cell
DOI 10.1016/s0092-8674(00)81433-6
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Abstract

To explore the role of cyclooxygenase (COX) in endothelial cell migration and angiogenesis, we have used two in vitro model systems involving coculture of endothelial cells with colon carcinoma cells. COX-2-overexpressing cells produce prostaglandins, proangiogenic factors, and stimulate both endothelial migration and tube formation, while control cells have little activity. The effect is inhibited by antibodies to combinations of angiogenic factors, by NS-398 (a selective COX-2 inhibitor), and by aspirin. NS-398 does not inhibit production of angiogenic factors or angiogenesis induced by COX-2-negative cells. Treatment of endothelial cells with aspirin or a COX-1 antisense oligonucleotide inhibits COX-1 activity/expression and suppresses tube formation. Cyclooxygenase regulates colon carcinoma-induced angiogenesis by two mechanisms: COX-2 can modulate production of angiogenic factors by colon cancer cells, while COX-1 regulates angiogenesis in endothelial cells.

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Crossref · 10.1016/s0092-8674(00)81433-6

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36 of 36 references verified0 citation errors · 0 not checkable

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  • HIF-1α-induced COX-2 expression leads to elevated PGE2 levels, which can induce PGE2-mediated vascularization.

    “HIF-1α-induced COX-2 expression leads to elevated PGE2 levels, which can induce PGE2-mediated vascularization [87,88].”

    From The Pathogenesis of Adenomyosis vis-à-vis Endometriosis (2020) · cites this paper as [88]

  • To ensure that the real-time PCR and Western blot analysis results refer to HESCs but not platelets, the HESCs co-cultured with platelets were washed with sterile PBS three times to remove the platelets.

    “To ensure that the real-time PCR and Western blot analysis results refer to HESCs but not platelets, we washed the HESCs that co-cultured with platelets with sterile PBS three times to remove the platelets as reported previously86.”

    From Platelets induce increased estrogen production through NF-κB and TGF-β1 signaling pathways in endometriotic stromal cells (2020) · cites this paper as [86]

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Authors
Tsujii, M., Kawano, S., Tsuji, S., Sawaoka, H., Hori, M., & DuBois, R. N.
Affiliations
  • Nashville VA Medical Center (US)
  • The University of Osaka (JP)
Corresponding author
DuBois, Raymond N
ORCID
Venue
Cell
Publisher
Elsevier BV
Published
1998-05
Volume
93
Issue
5
Pages
705-716
ISSN
0092-8674 (ISSN-L)
DOI
10.1016/s0092-8674(00)81433-6
OpenAlex
W2127558096
Cited by
2,272
References
36
Citations by year
2026: 7 · 2025: 8 · 2024: 15 · 2023: 17 · 2022: 18 · 2021: 32 · 2020: 33 · 2019: 36 · 2018: 21 · 2017: 40 · 2016: 40 · 2015: 47 · 2014: 49 · 2013: 64 · 2012: 63
FWCI
92.46
Type
journal article
Language
en
Open access
Licence
Locations
  • Cell (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Centers for Disease Control and Prevention (NIEHS-00267)
  • National Institute of Diabetes and Digestive and Kidney Diseases (DK47297)
Topics
  • Health Sciences › Medicine › Pharmacology › Inflammatory mediators and NSAID effects
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Angiogenesis and VEGF in Cancer
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Eicosanoids and Hypertension Pharmacology
Keywords
cyclooxygenase, angiogenesis, colon cancer, cyclooxygenase-2, cyclooxygenase-1, endothelial cell migration, tube formation, prostaglandins, angiogenic factors, NS-398, aspirin
MeSH terms
Animals, Anti-Inflammatory Agents, Non-Steroidal, Antineoplastic Agents, Carcinoma, Cell Communication, Cell Movement, Colonic Neoplasms, Endothelium, Vascular, Growth Substances, Humans, Isoenzymes, Membrane Proteins, Mice, Nude, Molecular Sequence Data, Neoplasms, Experimental, Neovascularization, Pathologic, Prostaglandin-Endoperoxide Synthases, Up-Regulation, Cyclooxygenase Inhibitors, Coculture Techniques, Caco-2 Cells, Mice, Cyclooxygenase 1, Cyclooxygenase 2, Cyclooxygenase 2 Inhibitors

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