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Cited by 270Muscle Physiology and DisordersDevelopmental Biology and Gene RegulationCardiomyopathy and Myosin Studies

The expression of myosin genes in developing skeletal muscle in the mouse embryo.

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Lyons, G E, Ontell, M, Cox, R, Sassoon, D, Buckingham, M·1990·The Journal of cell biology
DOI 10.1083/jcb.111.4.1465PMC PMC2116224
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Abstract

Using in situ hybridization, we have investigated the temporal sequence of myosin gene expression in the developing skeletal muscle masses of mouse embryos. The probes used were isoform-specific, 35S-labeled antisense cRNAs to the known sarcomeric myosin heavy chain and myosin alkali light chain gene transcripts. Results showed that both cardiac and skeletal myosin heavy chain and myosin light chain mRNAs were first detected between 9 and 10 d post coitum (p.c.) in the myotomes of the most rostral somites. Myosin transcripts appeared in more caudal somites at later stages in a developmental gradient. The earliest myosin heavy chain transcripts detected code for the embryonic skeletal (MHCemb) and beta-cardiac (MHC beta) isoforms. Perinatal myosin heavy chain (MHCpn) transcripts begin to accumulate at 10.5 d p.c., which is much earlier than previously reported. At this stage, MHCemb is the major MHC transcript. By 12.5 d p.c., MHCpn and MHCemb mRNAs are present to an equal extent, and by 15.5 d p.c. the MHCpn transcript is the major MHC mRNA detected. Cardiac MHC beta transcripts are always present as a minor component. In contrast, the cardiac MLC1A mRNA is initially more abundant than that encoding the skeletal MLC1F isoform. By 12.5 d p.c. the two MLC mRNAs are present at similar levels, and by 15.5 d p.c., MLC1F is the predominant MLC transcript detected. Transcripts for the ventricular/slow (MLC1V) and another fast skeletal myosin light chain (MLC3F) are not detected in skeletal muscle before 15 d p.c., which marks the beginning of the fetal stage of muscle development. This is the first stage at which we can detect differences in expression of myosin genes between developing muscle fibers. We conclude that, during the development of the myotome and body wall muscles, different myosin genes follow independent patterns of activation and accumulation. The data presented are the first detailed study of myosin gene expression at these early stages of skeletal muscle development.

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  • Efficient skeletal muscle-specific Tfam recombination has occurred before the age of 1 month.

    “The apparent absence of Tfam protein at the age of 1 month demonstrates that efficient skeletal musclespecific Tfam recombination has occurred before this age and is consistent with previous reports demonstrating that the Mlc1f promoter, which regulates cre-recombinase expression in skeletal muscle, is active from embryonic day 10 (22).”

    From Increased mitochondrial mass in mitochondrial myopathy mice (2002) · cites this paper as [22]

    The Results (p. 1467, Figs. 2–3) detect MLC1F transcripts in developing mouse muscle at 9.5 and 10.5 days post coitum, supporting early expression. The paper does not test whether the Mlc1f promoter drives Cre-recombinase or whether Tfam recombination occurred; the one-month recombination conclusion is the citing authors’ own inference. (LLM, conf=0.85)

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  • The Mlc1f promoter, which regulates cre-recombinase expression in skeletal muscle, is active from embryonic day 10.

    “consistent with previous reports demonstrating that the Mlc1f promoter, which regulates cre-recombinase expression in skeletal muscle, is active from embryonic day 10 (22).”

    From Increased mitochondrial mass in mitochondrial myopathy mice (2002) · cites this paper as [22]

    Figure 2 (p. 1467) reports detectable MLC1F transcripts in mouse myotomes at 9.5 days post coitum, and Figure 3 (p. 1468) shows them at 10.5 days. This supports early MLC1F gene expression, but the paper does not test whether an Mlc1f promoter drives Cre-recombinase expression from embryonic day 10. (LLM, conf=0.87)

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Authors
Lyons, G. E., Ontell, M., Cox, R., Sassoon, D., & Buckingham, M.
Affiliations
Centre National de la Recherche Scientifique (FR)
ORCID
Venue
The Journal of cell biology
Publisher
Rockefeller University Press
Published
1990-10-01
Volume
111
Issue
4
Pages
1465-1476
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.111.4.1465
PMC
PMC2116224
OpenAlex
W2082442359
Cited by
270
References
36
Citations by year
2026: 2 · 2024: 3 · 2023: 6 · 2022: 7 · 2021: 2 · 2020: 5 · 2019: 3 · 2018: 7 · 2017: 4 · 2016: 7 · 2015: 6 · 2014: 5 · 2013: 7 · 2012: 11
FWCI
6.41
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • The Journal of Cell Biology (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Muscle Physiology and Disorders
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Developmental Biology and Gene Regulation
  • Health Sciences › Medicine › Cardiology and Cardiovascular Medicine › Cardiomyopathy and Myosin Studies
Keywords
skeletal muscle development, myosin heavy chain, myosin light chain, embryonic myosin heavy chain, cardiac myosin heavy chain, myotome, somites, muscle fiber differentiation, mouse embryo, developmental gene regulation
MeSH terms
Animals, Base Sequence, Cell Differentiation, Gene Expression Regulation, Gestational Age, Mice, Inbred BALB C, Mice, Inbred C3H, Molecular Sequence Data, Muscles, Myosins, Nucleic Acid Hybridization, Phenotype, RNA, Messenger, Sulfur Radioisotopes, RNA Probes, Mice

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