The DOI matches its Crossref record; 27 of the 27 references it cites were found in a registry or an index.
Microtubule behavior in the growth cones of living neurons during axon elongation.
Open accessAbstract
To understand how microtubules are generated in the growth cone, we have imaged fluorescently tagged microtubules in living frog embryonic neurons. The neurons were labeled by injecting rhodamine-labeled tubulin into the fertilized egg and explanting the neurons from the neural tube. Microtubules extend deep into the growth cone periphery and adopt three characteristic distributions: (a) dispersed and splayed throughout much of the growth cone; (b) looped and apparently contorted by compression; and (c) bundled into tight arrays. These distributions interconvert on a time scale of several minutes and these interconversions are correlated with the behavior of the growth cone. We observed microtubule growth and shrinkage in growth cones, but are unable to determine their contribution to net assembly. However, translocation of polymer form the axon appears to be a major mechanism of generating new polymer in the growth cone, while bundling of microtubules in the growth cone appears to be the critical step in generating new axon. Neurons that were about to turn spontaneously generated microtubules in the future direction of growth, suggesting that orientation of microtubules might be an important early step in neuronal pathfinding.
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Cites
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Self-citationWalker, R. A., O'Brien, E. T., Pryer, N. K., Soboeiro, M. F., Voter, W. A., Erickson, H. P., & Salmon, E. D. (1988) · The Journal of Cell Biology · doi:10.1083/jcb.107.4.1437 · Cited by 980
Cytoskeletal dynamics and nerve growth
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Self-citationForscher, P., & Smith, S. J. (1988) · The Journal of cell biology · doi:10.1083/jcb.107.4.1505 · Cited by 854
ULTRASTRUCTURE AND FUNCTION OF GROWTH CONES AND AXONS OF CULTURED NERVE CELLS
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[39] Preparation of modified tubulins
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FINE STRUCTURE OF NERVE FIBERS AND GROWTH CONES OF ISOLATED SYMPATHETIC NEURONS IN CULTURE
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Real-time observations of microtubule dynamic instability in living cells.
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Cited by
MICROTUBULE POLYMERIZATION DYNAMICS
Desai, A., & Mitchison, T. J. (1997) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.13.1.83 · Cited by 2,563
Microtubule assembly, organization and dynamics in axons and dendrites
Conde, C., & Cáceres, A. (2009) · Nature Reviews Neuroscience · doi:10.1038/nrn2631 · Cited by 1,086
Cytoskeletal Dynamics and Transport in Growth Cone Motility and Axon Guidance
Dent, E. W., & Gertler, F. B. (2003) · Neuron · doi:10.1016/s0896-6273(03)00633-0 · Cited by 893
Contact and adhesive specificities in the associations, migrations, and targeting of cells and axons
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Local Nucleation of Microtubule Bundles through Tubulin Concentration into a Condensed Tau Phase
Hernández-Vega, A., Braun, M., Scharrel, L., Jahnel, M., Wegmann, S., Hyman, B. T., Alberti, S., Diez, S., & Hyman, A. A. (2017) · Cell Reports · doi:10.1016/j.celrep.2017.08.042 · Cited by 395
“Although there is considerable knowledge about how neurons use microtubule bundles for microtubule-based transport, we have less knowledge of how microtubule bundles form in the first place (Baas et al., 2005; Chen et al., 2017; Sanchez and Feldman, 2016; Tanaka and Kirschner, 1991).”
Background
Metadata
- Authors
- Tanaka, E. M., & Kirschner, M. W.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1991-10-15
- Volume
- 115
- Issue
- 2
- Pages
- 345-363
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W1973512372
- Cited by
- 347
- References
- 27
- FWCI
- 7.73
- Type
- journal article
- Language
- en
- Open access
- green
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
