The DOI matches its Crossref record; 61 of the 61 references it cites were found in a registry or an index.
Inhibition of lysophosphatidate- and thrombin-induced neurite retraction and neuronal cell rounding by ADP ribosylation of the small GTP-binding protein Rho.
Open accessAbstract
Addition of the bioactive phospholipid lysophosphatidic acid (LPA) or a thrombin receptor-activating peptide (TRP) to serum-starved N1E-115 or NG108-15 neuronal cells causes rapid growth cone collapse, neurite retraction, and transient rounding of the cell body. These shape changes appear to be driven by receptor-mediated contraction of the cortical actomyosin system independent of classic second messengers. Treatment of the cells with Clostridium botulinum C3 exoenzyme, which ADP-ribosylates and thereby inactivates the Rho small GTP-binding protein, inhibits LPA- and TRP-induced force generation and subsequent shape changes. C3 also inhibits LPA-induced neurite retraction in PC12 cells. Biochemical analysis reveals that the ADP-ribosylated substrate is RhoA. Prolonged C3 treatment of cells maintained in 10% serum induces the phenotype of serum-starved cells, with initial cell flattening being followed by neurite outgrowth; such C3-differentiated cells fail to retract their neurites in response to agonists. We conclude that RhoA is essential for receptor-mediated force generation and ensuing neurite retraction in N1E-115 and PC12 cells, and that inactivation of RhoA by ADP-ribosylation abolishes actomyosin contractility and promotes neurite outgrowth.
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Cites
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Cited by
Signaling from Rho to the Actin Cytoskeleton Through Protein Kinases ROCK and LIM-kinase
Maekawa, M., Ishizaki, T., Boku, S., Watanabe, N., Fujita, A., Iwamatsu, A., Obinata, T., Ohashi, K., Mizuno, K., & Narumiya, S. (1999) · Science · doi:10.1126/science.285.5429.895 · Cited by 1,636
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Actin Cytoskeleton Regulation in Neuronal Morphogenesis and Structural Plasticity
Luo, L. (2002) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.18.031802.150501 · Cited by 658
Lysophosphatidic Acid, a Multifunctional Phospholipid Messenger
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Metadata
- Authors
- Jalink, K., van Corven, E. J., Hengeveld, T., Morii, N., Narumiya, S., & Moolenaar, W. H.
- Venue
- The Journal of cell biology
- Publisher
- Rockefeller University Press
- Published
- 1994-08-01
- Volume
- 126
- Issue
- 3
- Pages
- 801-810
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2121666423
- Cited by
- 608
- References
- 61
- FWCI
- 9.80
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Inhibition of lysophosphatidate- and thrombin-induced neurite retraction and neuronal cell rounding by ADP ribosylation of the small GTP-binding protein Rho. https://app.cochat.ai/papers/10.1083/jcb.126.3.801
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- Jalink, K., van Corven, E. J., Hengeveld, T., Morii, N., Narumiya, S., & Moolenaar, W. H. (1994). Inhibition of lysophosphatidate- and thrombin-induced neurite retraction and neuronal cell rounding by ADP ribosylation of the small GTP-binding protein Rho. The Journal of cell biology. https://doi.org/10.1083/jcb.126.3.801
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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