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Cited by 541

The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.

Axonal transport of mitochondria along microtubules and F-actin in living vertebrate neurons.

Open access
Morris, R L, Hollenbeck, P J·1995·The Journal of cell biology
DOI 10.1083/jcb.131.5.1315
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Abstract

A large body of evidence indicates that microtubules (MTs) conduct organelle transport in axons, but recent studies on extruded squid axoplasm have suggested that actin microfilaments (MFs) may also play a role in this process. To investigate the separate contributions to transport of each class of cytoskeletal element in intact vertebrate axons, we have monitored mitochondrial movements in chick sympathetic neurons experimentally manipulated to eliminate MTs, MFs, or both. First, we grew neurons in the continuous presence of: (a) cytochalasin E to create neurites which had never contained MFs; or (b) nocodazole or vinblastine to produce neurites which had never contained MTs. Mitochondria moved bidirectionally at normal velocities along the length of neurites which contained MTs and lacked MFs, but did not even enter neurites grown without MTs but containing MFs. In a second approach, we treated established neuronal cultures with cytoskeletal drugs to disrupt either MTs or MFs in axons already containing mitochondria. In cytochalasin-treated cells, which retained MTs but lacked MFs, average mitochondrial velocity increased in both directions, but net directional transport decreased. In vinblastine-treated cells, which lacked MTs but retained essentially normal levels of MFs, mitochondria continued to move bidirectionally but the average mitochondrial velocity and excursion length were reduced for both directions of movement, and the mitochondria spent threefold as much time moving in the retrograde as in the anterograde direction, resulting in net retrograde transport. Treatment of established cultures with both drugs produced neurites lacking MTs and MFs but still rich in neurofilaments; these showed a striking absence of any mitochondrial motility. These data indicate that axonal organelle transport can occur along both MTs and MFs in vivo, but with different velocities and net transport properties.

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Metadata

Authors
Morris, R. L., & Hollenbeck, P. J.
Venue
The Journal of cell biology
Publisher
Rockefeller University Press
Published
1995-12-01
Volume
131
Issue
5
Pages
1315-1326
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.131.5.1315
OpenAlex
W2139861422
Cited by
541
References
1
FWCI
6.82
Type
journal article
Language
en
Open access
green

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