Choice of tracks, microtubules and/or actin filaments for chloroplast photo-movement is differentially controlled by phytochrome and a blue light receptor
Abstract
Light induced chloroplast movement has been studied as a model system for photoreception and actin microfilament (MF)-based intracellular motilities in plants. Chloroplast photo-accumulation and-avoidance movement is mediated by phytochrome as well as blue light (BL) receptor in the moss Physcomitrella patens. Here we report the discovery of an involvement of a microtubule (MT)-based system in addition to an MF-based system in photorelocation of chloroplasts in this moss. In the dark, MTs provided tracks for rapid movement of chloroplasts in a longitudinal direction and MFs contributed the tracks for slow movement in any direction. We found that phytochrome responses utilized only the MT-based system, while BL responses had an alternative way of moving, either along MTs or MFs. MT-based systems were mediated by both photoreceptors, but chloroplasts showed movements with different velocity and pattern between them. No apparent difference in the behavior of chloroplast movement between the accumulation and avoidance movement was detected in phytochrome responses or BL responses, except for the direction of the movement. The results presented here demonstrate that chloroplasts use both MTs and MFs for motility and that phytochrome and a BL receptor control directional photo-movement of chloroplasts through the differential regulation of these motile systems.
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- Authors
- Sato, Y., Wada, M., & Kadota, A.
- Affiliations
- Tokyo Metropolitan University (JP)
- National Institute for Basic Biology (JP)
- Venue
- Journal of Cell Science
- Publisher
- The Company of Biologists
- Published
- 2001-01-15
- Volume
- 114
- Issue
- 2
- Pages
- 269-279
- ISSN
- 0021-9533 (ISSN-L)
- 1477-9137
- OpenAlex
- W1949870098
- Cited by
- 148
- References
- 43
- Citations by year
- 2026: 2 · 2025: 2 · 2024: 1 · 2023: 3 · 2022: 7 · 2021: 2 · 2020: 3 · 2019: 3 · 2018: 3 · 2017: 9 · 2016: 6 · 2015: 6 · 2014: 3 · 2013: 4 · 2012: 9
- FWCI
- 11.82
- Type
- journal article
- Language
- en
- Open access
- closed
- Locations
- Journal of Cell Science (publishedVersion)
- PubMed (publishedVersion)
- HighWire Press Open Archive (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Light effects on plants
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Photosynthetic Processes and Mechanisms
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Molecular Biology Research
- Keywords
- phytochrome, blue light receptor, microtubules, F-actin, intracellular motility, bryophytes, Physcomitrella patens, directional motility, photoreception
- MeSH terms
- Actins, Chloroplasts, Cytochalasin B, Darkness, Light, Lighting, Microscopy, Fluorescence, Microtubules, Movement, Phytochrome, Phototropism, Bryopsida
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