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Activation of the MKK/ERK Pathway during Somatic Cell Mitosis: Direct Interactions of Active ERK with Kinetochores and Regulation of the Mitotic 3F3/2 Phosphoantigen

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Shapiro, Paul S., Vaisberg, Eugeni, Hunt, Alan J., Tolwinski, Nicholas S., Whalen, Anne M., McIntosh, J. Richard, Ahn, Natalie G.·1998·The Journal of Cell Biology
DOI 10.1083/jcb.142.6.1533
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Abstract

The mitogen-activated protein (MAP) kinase pathway, which includes extracellular signal–regulated protein kinases 1 and 2 (ERK1, ERK2) and MAP kinase kinases 1 and 2 (MKK1, MKK2), is well-known to be required for cell cycle progression from G1 to S phase, but its role in somatic cell mitosis has not been clearly established. We have examined the regulation of ERK and MKK in mammalian cells during mitosis using antibodies selective for active phosphorylated forms of these enzymes. In NIH 3T3 cells, both ERK and MKK are activated within the nucleus during early prophase; they localize to spindle poles between prophase and anaphase, and to the midbody during cytokinesis. During metaphase, active ERK is localized in the chromosome periphery, in contrast to active MKK, which shows clear chromosome exclusion. Prophase activation and spindle pole localization of active ERK and MKK are also observed in PtK1 cells. Discrete localization of active ERK at kinetochores is apparent by early prophase and during prometaphase with decreased staining on chromosomes aligned at the metaphase plate. The kinetochores of chromosomes displaced from the metaphase plate, or in microtubule-disrupted cells, still react strongly with the active ERK antibody. This pattern resembles that reported for the 3F3/2 monoclonal antibody, which recognizes a phosphoepitope that disappears with kinetochore attachment to the spindles, and has been implicated in the mitotic checkpoint for anaphase onset (Gorbsky and Ricketts, 1993. J. Cell Biol. 122:1311–1321). The 3F3/2 reactivity of kinetochores on isolated chromosomes decreases after dephosphorylation with protein phosphatase, and then increases after subsequent phosphorylation by purified active ERK or active MKK. These results suggest that the MAP kinase pathway has multiple functions during mitosis, helping to promote mitotic entry as well as targeting proteins that mediate mitotic progression in response to kinetochore attachment.

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Research works printed but not linked: 59

  1. Abrieu, 1996, MAP kinase does not inactivate, but rather prevents the cyclin degradation pathway from being turned on in Xenopusegg extracts, J Cell Sci, 109, 239, 10.1242/jcs.109.1.239 · doi:10.1242/jcs.109.1.239
  2. Abrieu, 1997, MAPK inactivation is required for the G2 to M-phase transition of the first mitotic cell cycle, EMBO (Eur Mol Biol Organ) J, 16, 6407, 10.1093/emboj/16.21.6407 · doi:10.1093/emboj/16.21.6407
  3. Belmont, 1996, Identification of a protein that interacts with tubulin dimers and increases the catastrophe rate of microtubules, Cell, 84, 623, 10.1016/S0092-8674(00)81037-5 · DOI · Paper page
  4. Beno, 1995, Protein kinase C and mitogen-activated protein kinase are required for 1,25-dihydroxy vitamin D3-stimulated Egr induction, J Biol Chem, 270, 3642, 10.1074/jbc.270.8.3642 · doi:10.1074/jbc.270.8.3642
  5. Brattsand, 1994, Cell-cycle-regulated phosphorylation of oncoprotein 18 on Ser18, Ser25, and Ser38, Eur J Biochem, 220, 359, 10.1111/j.1432-1033.1994.tb18632.x · doi:10.1111/j.1432-1033.1994.tb18632.x
  6. Campbell, 1995, Microinjection of mitotic cells with the 3F3/2 anti-phosphoepitope antibody delays the onset of anaphase, J Cell Biol, 129, 1195, 10.1083/jcb.129.5.1195 · DOI
  7. Cyert, 1988, Monoclonal antibodies specific for thiophosphorylated proteins recognize Xenopus MPF, Dev Biol, 129, 209, 10.1016/0012-1606(88)90175-3 · doi:10.1016/0012-1606(88)90175-3
  8. Drechsel, 1992, Modulation of the dynamic instability of tubulin assembly by the microtubule-associated protein tau, Mol Biol Cell, 3, 1141, 10.1091/mbc.3.10.1141 · DOI · Paper page
  9. Dudley, 1995, A synthetic inhibitor of the mitogen-activated protein kinase cascade, Proc Natl Acad Sci USA, 92, 7686, 10.1073/pnas.92.17.7686 · DOI · Paper page
  10. Edelmann, 1996, Cell cycle regulation of p70 S6 kinase and p42/p44 mitogen-activated protein kinases in Swiss mouse 3T3 fibroblasts, J Biol Chem, 271, 963, 10.1074/jbc.271.2.963 · DOI
  11. Ferrell, 1991, Cell cycle tyrosine phosphorylation of p34cdc2 and a microtubule-associated protein kinase homologue in Xenopusoocytes and eggs, Mol Cell Biol, 11, 1965
  12. Fukuda, 1996, Cytoplasmic localization of MAP kinase kinase directed by its N-terminal leucine-rich short amino acid sequence, which acts as a nuclear export signal, J Biol Chem, 271, 20024, 10.1074/jbc.271.33.20024 · DOI · Paper page
  13. Fukuda, 1997, CRM1 is responsible for intracellular transport mediated by the nuclear export signal, Nature, 390, 308, 10.1038/36894 · DOI · Paper page
  14. Gotoh, 1991, In vitro effects on microtubule dynamics of purified XenopusM phase-activated MAP kinase, Nature, 349, 251, 10.1038/349251a0 · DOI · Paper page
  15. Gorbsky, 1997, Cell cycle checkpoints: arresting progress in mitosis, Bioessays, 19, 193, 10.1002/bies.950190303 · DOI
  16. Gorbsky, 1993, Differential expression of a phosphoepitope at the kinetochores of moving chromosomes, J Cell Biol, 122, 1311, 10.1083/jcb.122.6.1311 · DOI · Paper page
  17. Haccard, 1993, Induction of metaphase arrest in cleaving Xenopus embryos by MAP kinase, Science, 262, 1262, 10.1126/science.8235656 · DOI · Paper page
  18. Hoshi, 1992, Mitogen-activated-protein-kinase-catalyzed phosphorylation of microtubule-associated proteins, microtubule-associated protein 2 and microtubule-associated protein 4, induces an alteration in their function, Eur J Biochem, 203, 43, 10.1111/j.1432-1033.1992.tb19825.x · DOI
  19. Hyman, 1993, An assay for the activity of microtubule-based motors on the kinetochores of isolated Chinese hamster ovary chromosomes, Methods Cell Biol, 39, 267, 10.1016/S0091-679X(08)60176-4 · doi:10.1016/s0091-679x(08)60176-4
  20. Jaaro, 1997, Nuclear translocation of mitogen-activated protein kinase kinase (MEK1) in response to mitogenic stimulation, Proc Natl Acad Sci USA, 94, 3742, 10.1073/pnas.94.8.3742 · DOI · Paper page
  21. Khokhlatchev, 1997, Reconstitution of mitogen-activated protein kinase phosphorylation cascades in bacteria, J Biol Chem, 272, 11057, 10.1074/jbc.272.17.11057 · DOI · Paper page
  22. Kortenjann, 1994, Inhibition of v-raf-dependent c-fos expression and transformation by a kinase-defective mutant of the mitogen-activated protein kinase ERK2, Mol Cell Biol, 14, 4815
  23. Kosako, 1994, Mitogen-activated protein kinase kinase is required for the mos-induced metaphase arrest, J Biol Chem, 269, 28354, 10.1016/S0021-9258(18)46935-9 · DOI
  24. Kotzin, 1984, Monoclonal anti-histone autoantibodies derived from murine models of lupus, J Immunol, 133, 2554, 10.4049/jimmunol.133.5.2554 · DOI
  25. Laird, 1995, Raf-1 is activated during mitosis, J Biol Chem, 270, 26742, 10.1074/jbc.270.45.26742 · DOI
  26. Larsson, 1995, G2/M transition requires multisite phosphorylation of oncoprotein 18 by two distinct protein kinase systems, J Biol Chem, 270, 14175, 10.1074/jbc.270.23.14175 · DOI
  27. Lavoie, 1996, Cyclin D1 expression is regulated positively by p42/p44mapk and negatively by the p38/HOGmapkpathway, J Biol Chem, 271, 20608, 10.1074/jbc.271.34.20608 · DOI · Paper page
  28. Lenormand, 1993, Growth factors induce nuclear translocation of MAP kinases (p42mapk and p44mapk) but not of their activator MAP kinase kinase (p45mapkk) in fibroblasts, J Cell Biol, 122, 1079, 10.1083/jcb.122.5.1079 · DOI · Paper page
  29. Lewis, 1997, Signal transduction through MAP kinase cascades, Adv Cancer Res, 74, 49, 10.1016/S0065-230X(08)60765-4 · DOI · Paper page
  30. Mandelkow, 1992, Glycogen synthase kinase-3 and the Alzheimer-like state of microtubule-associated protein tau, FEBS Lett, 314, 315, 10.1016/0014-5793(92)81496-9 · DOI · Paper page
  31. Mansour, 1996, Interdependent domains controlling the enzymatic activity of mitogen-activated protein kinase kinase 1, Biochemistry, 35, 15529, 10.1021/bi961854s · DOI
  32. Mansour, 1994, Mitogen-activated protein (MAP) kinase phosphorylation of MAP kinase kinase: determination of phosphorylation sites by mass spectrometry and site-directed mutagenesis, J Biochem, 116, 304, 10.1093/oxfordjournals.jbchem.a124524 · DOI
  33. Marklund, 1993, Serine 25 of oncoprotein 18 is a major cytosolic target for the mitogen-activated protein kinase, J Biol Chem, 268, 15039, 10.1016/S0021-9258(18)82435-8 · doi:10.1016/s0021-9258(18)82435-8
  34. McIntosh, 1991, Structural and mechanical control of mitotic progression, Cold Spring Harbor Symp Quant Biol, 56, 613, 10.1101/SQB.1991.056.01.070 · DOI
  35. Minshull, 1994, A MAP kinase-dependent spindle assembly checkpoint in Xenopus egg extracts, Cell, 79, 475, 10.1016/0092-8674(94)90256-9 · DOI · Paper page
  36. Morishima-Kawashima, 1996, The pool of MAP kinase associated with microtubules is small but constitutively active, Mol Biol Cell, 7, 893, 10.1091/mbc.7.6.893 · DOI
  37. Neighbors, 1988, Localization of kinesin in cultured cells, J Cell Biol, 106, 1193, 10.1083/jcb.106.4.1193 · DOI
  38. Nicklas, 1997, How cells get the right chromosome, Science, 275, 632, 10.1126/science.275.5300.632 · DOI · Paper page
  39. Nicklas, 1995, Kinetochore chemistry is sensitive to tension and may link mitotic forces to a cell cycle checkpoint, J Cell Biol, 130, 929, 10.1083/jcb.130.4.929 · DOI · Paper page
  40. Nigg, 1996, Dynamic changes in nuclear architecture during mitosis: on the role of protein phosphorylation in spindle assembly and chromosome segregation, Exp Cell Res, 229, 174, 10.1006/excr.1996.0356 · DOI
  41. Pagès, 1993, Mitogen-activated protein kinases p42mapk and p44mapkare required for fibroblast proliferation, Proc Natl Acad Sci USA, 90, 8319, 10.1073/pnas.90.18.8319 · DOI · Paper page
  42. Pathan, 1996, Activation of T cell Raf-1 at mitosis requires the protein-tyrosine kinase Lck, J Biol Chem, 271, 30315, 10.1074/jbc.271.48.30315 · doi:10.1074/jbc.271.48.30315
  43. Resing, 1998, Protein phosphorylation analysis by electrospray ionization-mass spectrometry, Methods Enzymol, 283, 29, 10.1016/S0076-6879(97)83005-4 · DOI
  44. Rezka, 1995, Association of mitogen-activated protein kinase with the microtubule cytoskeleton, Proc Natl Acad Sci USA, 92, 8881, 10.1073/pnas.92.19.8881 · DOI · Paper page
  45. Robbins, 1993, Regulation and properties of extracellular signal-regulated protein kinases 1 and 2 in vitro, J Biol Chem, 268, 5097, 10.1016/S0021-9258(18)53507-9 · DOI · Paper page
  46. Robinson, 1997, Mitogen-activated protein kinase pathways, Curr Opin Cell Biol, 9, 180, 10.1016/S0955-0674(97)80061-0 · DOI · Paper page
  47. Roche, 1995, Requirement for Src family protein tyrosine kinases in G2 for fibroblast cell division, Science, 269, 1567, 10.1126/science.7545311 · DOI · Paper page
  48. Sagata, 1997, What does Mos do in oocytes and somatic cells?, Bioessays, 19, 13, 10.1002/bies.950190105 · DOI · Paper page
  49. Seger, 1994, Overexpression of mitogen-activated protein kinase kinase (MAPKK) and its mutants in NIH 3T3 cells, J Biol Chem, 269, 25699, 10.1016/S0021-9258(18)47305-X · DOI · Paper page
  50. Shiina, 1992, Regulation of a major microtubule-associated protein by MPF and MAP kinase, EMBO (Eur Mol Biol Organ) J, 11, 3977, 10.1002/j.1460-2075.1992.tb05491.x · DOI
  51. Takenaka, 1998, Activation of the protein kinase p38 in the spindle assembly checkpoint and mitotic arrest, Science, 280, 599, 10.1126/science.280.5363.599 · DOI · Paper page
  52. Tamemoto, 1992, Biphasic activation of two mitogen-activated protein kinases during the cell cycle in mammalian cells, J Biol Chem, 267, 20293, 10.1016/S0021-9258(19)88700-8 · DOI
  53. Taylor, 1996, Cell cycle-dependent activation of Ras, Curr Biol, 6, 1621, 10.1016/S0960-9822(02)70785-9 · DOI · Paper page
  54. Verlhac, 1993, MAP kinase becomes stably activated at metaphase and is associated with microtubule-organizing centers during meiotic maturation of mouse oocytes, Dev Biol, 158, 330, 10.1006/dbio.1993.1192 · DOI · Paper page
  55. Walter, 1997, Induction of a G2-phase arrest in Xenopus egg extracts by activation of p42 mitogen-activated protein kinase, Mol Biol Cell, 8, 2157, 10.1091/mbc.8.11.2157 · DOI
  56. Wang, 1997, A role for mitogen-activated protein kinase in the spindle assembly checkpoint in XTC cells, J Cell Biol, 137, 433, 10.1083/jcb.137.2.433 · DOI
  57. Yen, 1991, CENP-E, a novel centromere associated protein required for progression from metaphase to anaphase, EMBO (Eur Mol Biol Organ) J, 10, 1245, 10.1002/j.1460-2075.1991.tb08066.x · DOI · Paper page
  58. Yew, 1993, Mos and the cell cycle: the molecular basis of the transformed phenotype, Curr Opin Genet Dev, 3, 19, 10.1016/S0959-437X(05)80336-3 · doi:10.1016/s0959-437x(05)80336-3
  59. Yung, 1997, Detection of ERK activation by a novel monoclonal antibody, FEBS Lett, 408, 292, 10.1016/S0014-5793(97)00442-0 · DOI

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Metadata

Authors
Shapiro, P. S., Vaisberg, E., Hunt, A. J., Tolwinski, N. S., Whalen, A. M., McIntosh, J. R., & Ahn, N. G.
Venue
The Journal of Cell Biology
Publisher
Rockefeller University Press
Published
1998-09-21
Volume
142
Issue
6
Pages
1533-1545
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.142.6.1533
OpenAlex
W2020099509
Cited by
224
References
1
FWCI
7.72
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Activation of the MKK/ERK Pathway during Somatic Cell Mitosis: Direct Interactions of Active ERK with Kinetochores and Regulation of the Mitotic 3F3/2 Phosphoantigen. https://app.cochat.ai/papers/10.1083/jcb.142.6.1533
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Shapiro, P. S., Vaisberg, E., Hunt, A. J., Tolwinski, N. S., Whalen, A. M., McIntosh, J. R., & Ahn, N. G. (1998). Activation of the MKK/ERK Pathway during Somatic Cell Mitosis: Direct Interactions of Active ERK with Kinetochores and Regulation of the Mitotic 3F3/2 Phosphoantigen. The Journal of Cell Biology. https://doi.org/10.1083/jcb.142.6.1533

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