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Cited by 286Cellular transport and secretionAutophagy in Disease and TherapyExtracellular vesicles in disease

Syntaxin 13 Mediates Cycling of Plasma Membrane Proteins via Tubulovesicular Recycling Endosomes

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Prekeris, Rytis, Klumperman, Judith, Chen, Yu A., Scheller, Richard H.·1998·The Journal of Cell Biology
DOI 10.1083/jcb.143.4.957PMC PMC2132958
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Abstract

Endocytosis-mediated recycling of plasma membrane is a critical vesicle trafficking step important in diverse biological processes. The membrane trafficking decisions and sorting events take place in a series of heterogeneous and highly dynamic organelles, the endosomes. Syntaxin 13, a recently discovered member of the syntaxin family, has been suggested to play a role in mediating endosomal trafficking. To better understand the function of syntaxin 13 we examined its intracellular distribution in nonpolarized cells. By confocal immunofluorescence and electron microscopy, syntaxin 13 is primarily found in tubular early and recycling endosomes, where it colocalizes with transferrin receptor. Additional labeling is also present in endosomal vacuoles, where it is often found in clathrin-coated membrane areas. Furthermore, anti-syntaxin 13 antibody inhibits transferrin receptor recycling in permeabilized PC12 cells. Immunoprecipitation of syntaxin 13 revealed that, in Triton X-100 extracts, syntaxin 13 is present in a complex(es) comprised of βSNAP, VAMP 2/3, and SNAP-25. This complex(es) binds exogenously added αSNAP and NSF and dissociates in the presence of ATP, but not ATPγS. These results support a role for syntaxin 13 in membrane fusion events during the recycling of plasma membrane proteins.

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  • Syntaxin 13 resides in tubular structures that connect the early and recycling endosomes and have been suggested to mediate protein recycling via early/recycling tubulovesicular endosomes enriched in Rab11 endosomes.

    “Syntaxin 13 resides in tubular structures that connect the early and recycling endosomes and have been suggested to mediate protein recycling via early/recycling tubulovesicular endosomes that are enriched in Rab11 endosomes [70], [71].”

    From Sorting nexin 17 regulates ApoER2 recycling and reelin signaling (2014) · cites this paper as [70]

    The Results and Discussion (pages not specified in our copy) place syntaxin 13 on a tubulovesicular recycling network that includes early- and recycling-endosome tubules, and the Discussion suggests a role in protein recycling through that network. The paper describes Rab11 as thought to be specific to recycling endosomes, but does not show that the syntaxin 13–positive structures are enriched in Rab11 or directly connect the two endosome types. (LLM, conf=0.86)

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Cited by

Sorted by citations

Authors
Prekeris, R., Klumperman, J., Chen, Y. A., & Scheller, R. H.
Affiliations
Howard Hughes Medical Institute (US)
ORCID
Venue
The Journal of Cell Biology
Publisher
Rockefeller University Press
Published
1998-11-16
Volume
143
Issue
4
Pages
957-971
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.143.4.957
PMC
PMC2132958
OpenAlex
W1979688230
Cited by
286
References
1
Citations by year
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FWCI
6.42
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • The Journal of Cell Biology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by-nc-sa)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
Keywords
recycling endosomes, endosomal trafficking, transferrin receptor recycling, membrane fusion, SNARE proteins, SNAP-25, NSF, vesicle trafficking
MeSH terms
Adenosine Triphosphate, Animals, Antineoplastic Agents, Biological Transport, Carrier Proteins, Cell Membrane, Cricetinae, Kidney, Ligands, Membrane Proteins, Microscopy, Electron, Nerve Tissue Proteins, Neurons, Protein Synthesis Inhibitors, Endosomes, RNA, Messenger, Synaptic Vesicles, Nocodazole, Gene Expression, CHO Cells, 3T3 Cells, PC12 Cells, Brefeldin A, Vesicular Transport Proteins, R-SNARE Proteins, Qa-SNARE Proteins, Mice, Rats, N-Ethylmaleimide-Sensitive Proteins, Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins

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