Cited by 91Cellular transport and secretionAutophagy in Disease and TherapyCalcium signaling and nucleotide metabolism

Syntaxin 7 Complexes with Mouse Vps10p Tail Interactor 1b, Syntaxin 6, Vesicle-associated Membrane Protein (VAMP)8, and VAMP7 in B16 Melanoma Cells

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Wade, Nick, Bryant, Nia J., Connolly, Lisa M., Simpson, Richard J., Luzio, J. Paul, Piper, Robert C., James, David E.·2001·Journal of Biological Chemistry

Abstract

Syntaxin 7 is a mammalian target soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) involved in membrane transport between late endosomes and lysosomes. The aim of the present study was to use immunoaffinity techniques to identify proteins that interact with Syntaxin 7. We reasoned that this would be facilitated by the use of cells producing high levels of Syntaxin 7. Screening of a large number of tissues and cell lines revealed that Syntaxin 7 is expressed at very high levels in B16 melanoma cells. Moreover, the expression of Syntaxin 7 increased in these cells as they underwent melanogenesis. From a large scale Syntaxin 7 immunoprecipitation, we have identified six polypeptides using a combination of electrospray mass spectrometry and immunoblotting. These polypeptides corresponded to Syntaxin 7, Syntaxin 6, mouse Vps10p tail interactor 1b (mVti1b), alpha-synaptosome-associated protein (SNAP), vesicle-associated membrane protein (VAMP)8, VAMP7, and the protein phosphatase 1M regulatory subunit. We also observed partial colocalization between Syntaxin 6 and Syntaxin 7, between Syntaxin 6 and mVti1b, but not between Syntaxin 6 and the early endosomal t-SNARE Syntaxin 13. Based on these and data reported previously, we propose that Syntaxin 7/mVti1b/Syntaxin 6 may form discrete SNARE complexes with either VAMP7 or VAMP8 to regulate fusion events within the late endosomal pathway and that these events may play a critical role in melanogenesis.

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  • This complex brings endosomes/phagosomes in close proximity as a prerequisite for fusion, probably involving VAMP7 and 8.

    “This complex brings endosomes/phagosomes in close proximity as a prerequisite for fusion (probably involving VAMP7 and 8) (35, 36).”

    From Macrophage defense mechanisms against intracellular bacteria (2015) · cites this paper as [35]

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  1. Yang, W., and Desiderio, S. (1997) Proc. Natl. Acad. Sci. U. S. A. 94, 604 -609
  2. Wilson, S. M., Yip, R., Swing, D. A., O'Sullivan, T. N., Zhang, Y., Novak, E. K., Swant, R. T., Russell, L. B., Copeland, N. G., and Jenkins, N. A.. (2000) Proc. Natl. Acad. Sci. U. S. A. 97, 7933-7938

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Authors
Wade, N., Bryant, N. J., Connolly, L. M., Simpson, R. J., Luzio, J. P., Piper, R. C., & James, D. E.
Affiliations
  • The University of Queensland (AU)
  • Walter and Eliza Hall Institute of Medical Research (AU)
  • University of Cambridge (GB)
  • University of Iowa (US)
Corresponding author
James, David E.
Venue
Journal of Biological Chemistry
Publisher
Elsevier BV
Published
2001-01
Volume
276
Issue
23
Pages
19820-19827
ISSN
0021-9258 (ISSN-L)
OpenAlex
W2002982584
Cited by
89
References
46
Citations by year
2025: 3 · 2023: 3 · 2022: 3 · 2021: 4 · 2020: 3 · 2019: 2 · 2018: 2 · 2017: 4 · 2016: 4 · 2015: 4 · 2013: 1 · 2012: 2
FWCI
2.61
Type
journal article
Language
en
Open access
Locations
  • Journal of Biological Chemistry (publishedVersion, cc-by)
  • PubMed (publishedVersion)
Funders
  • American Heart Association
  • Australian Research Council
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
  • Health Sciences › Medicine › Epidemiology › Autophagy in Disease and Therapy
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Physiology › Calcium signaling and nucleotide metabolism
Keywords
syntaxin 7, Vti1b, syntaxin 6, VAMP8, VAMP7, SNARE complex, protein-protein interaction, melanogenesis, B16 melanoma, t-SNARE
MeSH terms
Amino Acid Sequence, Animals, Antibody Specificity, Carrier Proteins, Melanoma, Experimental, Membrane Proteins, Molecular Sequence Data, Precipitin Tests, Protein Binding, Tumor Cells, Cultured, R-SNARE Proteins, Qa-SNARE Proteins, Qb-SNARE Proteins, Mice

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