The DOI matches its Crossref record; 49 of the 49 references it cites were found in a registry or an index.
Location and Clonal Analysis of Stem Cells and Their Differentiated Progeny in the Human Ocular Surface
Open accessAbstract
We have analyzed the proliferative and differentiation potential of human ocular keratinocytes. Holoclones, meroclones, and paraclones, previously identified in skin, constitute also the proliferative compartment of the ocular epithelium. Ocular holoclones have the expected properties of stem cells, while transient amplifying cells have variable proliferative potential. Corneal stem cells are segregated in the limbus, while conjunctival stem cells are uniformly distributed in bulbar and forniceal conjunctiva. Conjunctival keratinocytes and goblet cells derive from a common bipotent progenitor. Goblet cells were found in cultures of transient amplifying cells, suggesting that commitment for goblet cell differentiation can occur late in the life of a single conjunctival clone. We found that conjunctival keratinocytes with high proliferative capacity give rise to goblet cells at least twice in their life and, more importantly, at rather precise times of their life history, namely at 45–50 cell doublings and at ∼15 cell doublings before senescence. Thus, the decision of conjunctival keratinocytes to differentiate into goblet cells appears to be dependent upon an intrinsic “cell doubling clock.” These data open new perspectives in the surgical treatment of severe defects of the anterior ocular surface with autologous cultured conjunctival epithelium.
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Cites
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Cited by
Involvement of Follicular Stem Cells in Forming Not Only the Follicle but Also the Epidermis
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Regeneration of the entire human epidermis using transgenic stem cells
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Self-citationClonogenicity of Human Endometrial Epithelial and Stromal Cells1
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Oligopotent stem cells are distributed throughout the mammalian ocular surface
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Metadata
- Authors
- Pellegrini, G., Golisano, O., Paterna, P., Lambiase, A., Bonini, S., Rama, P., & De Luca, M.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1999-05-17
- Volume
- 145
- Issue
- 4
- Pages
- 769-782
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2126917358
- Cited by
- 722
- References
- 49
- FWCI
- 12.15
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Pellegrini, G., Golisano, O., Paterna, P., Lambiase, A., Bonini, S., Rama, P., & De Luca, M. (1999). Location and Clonal Analysis of Stem Cells and Their Differentiated Progeny in the Human Ocular Surface. The Journal of Cell Biology. https://doi.org/10.1083/jcb.145.4.769
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
