The DOI matches its Crossref record; 2 of the 2 references it cites were found in a registry or an index.
Cytoplasmic Dynein Is Required for Distinct Aspects of Mtoc Positioning, Including Centrosome Separation, in the One Cell Stage Caenorhabditis elegans Embryo
Open accessAbstract
We have investigated the role of cytoplasmic dynein in microtubule organizing center (MTOC) positioning using RNA-mediated interference (RNAi) in Caenorhabditis elegans to deplete the product of the dynein heavy chain gene dhc-1. Analysis with time-lapse differential interference contrast microscopy and indirect immunofluorescence revealed that pronuclear migration and centrosome separation failed in one cell stage dhc-1 (RNAi) embryos. These phenotypes were also observed when the dynactin components p50/dynamitin or p150Glued were depleted with RNAi. Moreover, in 15% of dhc-1 (RNAi) embryos, centrosomes failed to remain in proximity of the male pronucleus. When dynein heavy chain function was diminished only partially with RNAi, centrosome separation took place, but orientation of the mitotic spindle was defective. Therefore, cytoplasmic dynein is required for multiple aspects of MTOC positioning in the one cell stage C. elegans embryo. In conjunction with our observation of cytoplasmic dynein distribution at the periphery of nuclei, these results lead us to propose a mechanism in which cytoplasmic dynein anchored on the nucleus drives centrosome separation.
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Cites
Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans
Fire, A., Xu, S., Montgomery, M. K., Kostas, S. A., Driver, S. E., & Mello, C. C. (1998) · Nature · doi:10.1038/35888 · Cited by 15,429
The embryonic cell lineage of the nematode Caenorhabditis elegans
Sulston, J. E., Schierenberg, E., White, J. G., & Thomson, J. N. (1983) · Developmental Biology · doi:10.1016/0012-1606(83)90201-4 · Cited by 4,199
Cited by
Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans
Sönnichsen, B., Koski, L. B., Walsh, A., Marschall, P., Neumann, B., Brehm, M., Alleaume, A.-M., Artelt, J., Bettencourt, P., Cassin, E., et al. (2005) · Nature · doi:10.1038/nature03353 · Cited by 894
Self-citationConserved microtubule–actin interactions in cell movement and morphogenesis
Rodriguez, O. C., Schaefer, A. W., Mandato, C. A., Forscher, P., Bement, W. M., & Waterman-Storer, C. M. (2003) · Nature Cell Biology · doi:10.1038/ncb0703-599 · Cited by 844
The nuclear lamina comes of age
Gruenbaum, Y., Margalit, A., Goldman, R. D., Shumaker, D. K., & Wilson, K. L. (2005) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm1550 · Cited by 834
The cytoplasmic dynein transport machinery and its many cargoes
Reck-Peterson, S. L., Redwine, W. B., Vale, R. D., & Carter, A. P. (2018) · Nature Reviews Molecular Cell Biology · doi:10.1038/s41580-018-0004-3 · Cited by 659
Dare to Be Different: Asymmetric Cell Division in Drosophila, C. elegans and Vertebrates
Betschinger, J., & Knoblich, J. A. (2004) · Current Biology · doi:10.1016/j.cub.2004.08.017 · Cited by 448
Metadata
- Authors
- Gönczy, P., Pichler, S., Kirkham, M., & Hyman, A. A.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1999-10-04
- Volume
- 147
- Issue
- 1
- Pages
- 135-150
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2049469649
- Cited by
- 447
- References
- 2
- FWCI
- 7.68
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Cytoplasmic Dynein Is Required for Distinct Aspects of Mtoc Positioning, Including Centrosome Separation, in the One Cell Stage Caenorhabditis elegans Embryo. https://app.cochat.ai/papers/10.1083/jcb.147.1.135
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- Gönczy, P., Pichler, S., Kirkham, M., & Hyman, A. A. (1999). Cytoplasmic Dynein Is Required for Distinct Aspects of Mtoc Positioning, Including Centrosome Separation, in the One Cell Stage Caenorhabditis elegans Embryo. The Journal of Cell Biology. https://doi.org/10.1083/jcb.147.1.135
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