The DOI matches its Crossref record; 51 of the 51 references it cites were found in a registry or an index.
Human Cyclin a Is Required for Mitosis until Mid Prophase
Open accessAbstract
We have used microinjection and time-lapse video microscopy to study the role of cyclin A in mitosis. We have injected purified, active cyclin A/cyclin-dependent kinase 2 (CDK2) into synchronized cells at specific points in the cell cycle and assayed its effect on cell division. We find that cyclin A/CDK2 will drive G2 phase cells into mitosis within 30 min of microinjection, up to 4 h before control cells enter mitosis. Often this premature mitosis is abnormal; the chromosomes do not completely condense and daughter cells fuse. Remarkably, microinjecting cyclin A/CDK2 into S phase cells has no effect on progress through the following G2 phase or mitosis. In complementary experiments we have microinjected the amino terminus of p21Cip1/Waf1/Sdi1 (p21N) into cells to inhibit cyclin A/CDK2 activity. We find that p21N will prevent S phase or G2 phase cells from entering mitosis, and will cause early prophase cells to return to interphase. These results suggest that cyclin A/CDK2 is a rate-limiting component required for entry into mitosis, and for progress through mitosis until late prophase. They also suggest that cyclin A/CDK2 may be the target of the recently described prophase checkpoint.
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Cyclin A is required at two points in the human cell cycle.
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Inhibition of cyclin-dependent kinases by p21.
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Self-citationCyclin a is required for the onset of DNA replication in mammalian fibroblasts
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Cited by
Stuck in Division or Passing through
Rieder, C. L., & Maiato, H. (2004) · Developmental Cell · doi:10.1016/j.devcel.2004.09.002 · Cited by 657
Anand, S., Penrhyn-Lowe, S., & Venkitaraman, A. R. (2003) · Cancer Cell · doi:10.1016/s1535-6108(02)00235-0 · Cited by 631
The decision to enter mitosis: feedback and redundancy in the mitotic entry network
Lindqvist, A., Rodríguez-Bravo, V., & Medema, R. H. (2009) · Journal of Cell Biology · doi:10.1083/jcb.200812045 · Cited by 565
Inhibitors of cyclin-dependent kinases as cancer therapeutics.
Whittaker, S. R., Mallinger, A., Workman, P., & Clarke, P. A. (2017) · Pharmacology & Therapeutics · doi:10.1016/j.pharmthera.2017.02.008 · Cited by 384
Cyclin a Is Destroyed in Prometaphase and Can Delay Chromosome Alignment and Anaphase
den Elzen, N., & Pines, J. (2001) · The Journal of Cell Biology · doi:10.1083/jcb.153.1.121 · Cited by 371
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Metadata
- Authors
- Furuno, N., den Elzen, N., & Pines, J.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1999-10-18
- Volume
- 147
- Issue
- 2
- Pages
- 295-306
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2089975983
- Cited by
- 261
- References
- 51
- FWCI
- 4.90
- Type
- journal article
- Language
- en
- Open access
- green
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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