The DOI matches its Crossref record.
Imaging Constitutive Exocytosis with Total Internal Reflection Fluorescence Microscopy
Open accessAbstract
Total internal reflection fluorescence microscopy has been applied to image the final stage of constitutive exocytosis, which is the fusion of single post-Golgi carriers with the plasma membrane. The use of a membrane protein tagged with green fluorescent protein allowed the kinetics of fusion to be followed with a time resolution of 30 frames/s. Quantitative analysis allowed carriers undergoing fusion to be easily distinguished from carriers moving perpendicularly to the plasma membrane. The flattening of the carriers into the plasma membrane is seen as a simultaneous rise in the total, peak, and width of the fluorescence intensity. The duration of this flattening process depends on the size of the carriers, distinguishing small spherical from large tubular carriers. The spread of the membrane protein into the plasma membrane upon fusion is diffusive. Mapping many fusion sites of a single cell reveals that there are no preferred sites for constitutive exocytosis in this system.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Multicolour imaging of post-Golgi sorting and trafficking in live cells
Keller, P., Toomre, D., Díaz, E., White, J., & Simons, K. (2001) · Nature Cell Biology · doi:10.1038/35055042 · Cited by 398
Rab11 in Recycling Endosomes Regulates the Sorting and Basolateral Transport of E-Cadherin
Lock, J. G., & Stow, J. L. (2005) · Molecular Biology of the Cell · doi:10.1091/mbc.e04-10-0867 · Cited by 389
Chow, A., Toomre, D., Garrett, W., & Mellman, I. (2002) · Nature · doi:10.1038/nature01006 · Cited by 375
Rab6 regulates transport and targeting of exocytotic carriers
Grigoriev, I., Splinter, D., Keijzer, N., Wulf, P. S., Demmers, J., Ohtsuka, T., Modesti, M., Maly, I. V., Grosveld, F., Hoogenraad, C. C., et al. (2007) · Developmental Cell · doi:10.1016/j.devcel.2007.06.010 · Cited by 361
Plasma Membrane Is the Site of Productive HIV-1 Particle Assembly
Jouvenet, N., Neil, S. J. D., Bess, C., Johnson, M. C., Virgen, C. A., Simon, S. M., & Bieniasz, P. D. (2006) · PLoS Biology · doi:10.1371/journal.pbio.0040435 · Cited by 357
Self-citation
Metadata
- Authors
- Schmoranzer, J., Goulian, M., Axelrod, D., & Simon, S. M.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2000-04-03
- Volume
- 149
- Issue
- 1
- Pages
- 23-32
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2086489288
- Cited by
- 190
- References
- 0
- FWCI
- 6.40
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Imaging Constitutive Exocytosis with Total Internal Reflection Fluorescence Microscopy. https://app.cochat.ai/papers/10.1083/jcb.149.1.23
- The paper
- Schmoranzer, J., Goulian, M., Axelrod, D., & Simon, S. M. (2000). Imaging Constitutive Exocytosis with Total Internal Reflection Fluorescence Microscopy. The Journal of Cell Biology. https://doi.org/10.1083/jcb.149.1.23
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
