Rab6 regulates transport and targeting of exocytotic carriers
Open accessAbstract
Constitutive exocytosis delivers newly synthesized proteins, lipids, and other molecules from the Golgi apparatus to the cell surface. This process is mediated by vesicles, which bud off the trans-Golgi network, move along cytoskeletal filaments, and fuse with the plasma membrane. Here, we show that the small GTPase Rab6 marks exocytotic vesicles and, together with the microtubule plus-end-directed motor kinesin-1, stimulates their processive microtubule-based transport to the cell periphery. Furthermore, Rab6 directs targeting of secretory vesicles to plasma-membrane sites enriched in the cortical protein ELKS, a known Rab6 binding partner. Our data demonstrate that although Rab6 is not essential for secretion, it controls the organization of exocytosis within the cellular space.
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Rab6A participates in the motility of post-Golgi, exocytic transport vesicles in cooperation with KIF5B and with cytoplasmic dynein via bicaudal D1 and D2 proteins.
“Rab6A participates in the motility of post-Golgi, exocytic transport vesicles (Grigoriev et al., 2007) in cooperation with the kinesin motor, KIF5B, and with cytoplasmic dynein via its effector, bicaudal D1 and D2 proteins (Matanis et al., 2002).”
From The Rab6-regulated KIF1C kinesin motor domain contributes to Golgi organization (2015) · cites this paper as Grigoriev et al., 2007
Depletion of KIF5B did not fully block the motility of Rab6A vesicles in previous work.
“Depletion of KIF5B did not fully block the motility of Rab6A vesicles in previous work (Grigoriev et al., 2007), thus, KIF1C likely functions in conjunction with these motors to drive Rab6A vesicle motility.”
From The Rab6-regulated KIF1C kinesin motor domain contributes to Golgi organization (2015) · cites this paper as Grigoriev et al., 2007
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The Rab6-regulated KIF1C kinesin motor domain contributes to Golgi organization
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- Authors
- Grigoriev, I., Splinter, D., Keijzer, N., Wulf, P. S., Demmers, J., Ohtsuka, T., Modesti, M., Maly, I. V., Grosveld, F., Hoogenraad, C. C., & Akhmanova, A.
- Affiliations
- Erasmus MC (NL)
- University of Toyama (JP)
- University of Pittsburgh (US)
- Corresponding author
- Akhmanova, Anna
- ORCID
- Venue
- Developmental Cell
- Publisher
- Elsevier BV
- Published
- 2007-08
- Volume
- 13
- Issue
- 2
- Pages
- 305-314
- ISSN
- 1534-5807 (ISSN-L)
- OpenAlex
- W2091205354
- Cited by
- 361
- References
- 36
- Citations by year
- 2026: 9 · 2025: 23 · 2024: 24 · 2023: 20 · 2022: 17 · 2021: 23 · 2020: 27 · 2019: 23 · 2018: 16 · 2017: 24 · 2016: 20 · 2015: 10 · 2014: 24 · 2013: 23 · 2012: 20
- FWCI
- 5.50
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Licence
- www.elsevier.com/tdm/userlicense/1.0/ (tdm)
- www.elsevier.com/legal/tdmrep-license (tdm)
- www.elsevier.com/open-access/userlicense/1.0/ (vor, 2213 d)
- doi.org/10.15223/policy-017 (stm-asf)
- doi.org/10.15223/policy-037 (stm-asf)
- doi.org/10.15223/policy-012 (stm-asf)
- doi.org/10.15223/policy-029 (stm-asf)
- doi.org/10.15223/policy-004 (stm-asf)
- Locations
- Developmental Cell (publishedVersion)
- PubMed (publishedVersion)
- EUR Research Repository (Erasmus University Rotterdam) (publishedVersion)
- EUR Research Repository (Erasmus University Rotterdam) (submittedVersion)
- Funders
- European Science Foundation
- Nederlandse Organisatie voor Wetenschappelijk Onderzoek (814.02.005)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Extracellular vesicles in disease
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Keywords
- Rab6, secretory vesicle transport, microtubule transport, kinesin-1, trans-Golgi network, Golgi apparatus, elk, plasma membrane targeting, small GTPases, vesicle fusion
- MeSH terms
- Animals, Biological Transport, Cell Line, Cell Membrane, Cytoplasm, Dogs, Dyneins, Exocytosis, HeLa Cells, Humans, Membrane Fusion, Nerve Tissue Proteins, Biomarkers, Kinesins, rab GTP-Binding Proteins, Transport Vesicles, Adaptor Proteins, Signal Transducing, Rats
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