Cited by 421Adipose Tissue and MetabolismLipid metabolism and biosynthesisPeroxisome Proliferator-Activated Receptors

THE DIFFERENTIATION OF WHITE ADIPOSE CELLS

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Napolitano, Leonard·1963·The Journal of Cell Biology

Abstract

Differentiating white adipose tissue from presumptive and developing fat pads of newborn and young rats was fixed in buffered osmium tetroxide, embedded in Vestopal W, and examined in an electron microscope. Pre-adipose cells were found to be fibroblasts characterized by their spindle shape, long tenuous cytoplasmic extensions, and profuse endoplasmic reticulum. The developmental stages traced from fibroblast to mature adipose cell show a gradual change in cell shape, an accumulation of cytoplasm and non-membrane-bounded lipid, a decrease in the endoplasmic reticulum, and a change in shape of mitochondria. Transitory glycogen appears at mid-differentiation. Numerous smooth-membraned vesicles occur in the cytoplasm throughout differentiation. Pinocytosis is constantly evident. Cells of the multilocular stage are shown to differ from brown fat cells, particularly with respect to cytoplasmic membrane systems and mitochondria. No transport of particulate lipid from the lumen of the capillary to, or within, the adipose cell was detected, nor could any cell organelle be demonstrated to be visibly related to lipid synthesis and/or deposition.

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  • Adipocytes are enmeshed in a dense network of ECM.

    “Adipocytes are enmeshed in a dense network of ECM (100, 101)”

    From Adipose tissue remodeling and obesity (2011) · cites this paper as [100]

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Authors
Napolitano, L.
Affiliations
University of Pittsburgh (US)
Corresponding author
Napolitano, Leonard
Venue
The Journal of Cell Biology
Published
1963-09-01
Volume
18
Issue
3
Pages
663-679
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
OpenAlex
W1990614859
Cited by
421
References
14
Citations by year
2026: 1 · 2025: 5 · 2024: 6 · 2023: 5 · 2022: 4 · 2021: 4 · 2020: 1 · 2019: 5 · 2018: 10 · 2017: 5 · 2016: 10 · 2015: 8 · 2014: 10 · 2013: 17 · 2012: 12
FWCI
58.72
Type
journal article
Language
en
Open access
Locations
  • The Journal of Cell Biology (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Funders
U.S. Public Health Service
Topics
  • Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
Keywords
white adipocyte differentiation, fibroblasts, adipocyte development, endoplasmic reticulum, mitochondria, glycogen, pinocytosis, brown adipocytes, lipid accumulation, lipogenesis
MeSH terms
Adipose Tissue, Animals, Capillaries, Cell Differentiation, Cell Membrane, Cytoplasm, Electrons, Endoplasmic Reticulum, Fibroblasts, Glycogen, Microscopy, Microscopy, Electron, Mitochondria, Pinocytosis, Rats

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