THE DIFFERENTIATION OF WHITE ADIPOSE CELLS
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Differentiating white adipose tissue from presumptive and developing fat pads of newborn and young rats was fixed in buffered osmium tetroxide, embedded in Vestopal W, and examined in an electron microscope. Pre-adipose cells were found to be fibroblasts characterized by their spindle shape, long tenuous cytoplasmic extensions, and profuse endoplasmic reticulum. The developmental stages traced from fibroblast to mature adipose cell show a gradual change in cell shape, an accumulation of cytoplasm and non-membrane-bounded lipid, a decrease in the endoplasmic reticulum, and a change in shape of mitochondria. Transitory glycogen appears at mid-differentiation. Numerous smooth-membraned vesicles occur in the cytoplasm throughout differentiation. Pinocytosis is constantly evident. Cells of the multilocular stage are shown to differ from brown fat cells, particularly with respect to cytoplasmic membrane systems and mitochondria. No transport of particulate lipid from the lumen of the capillary to, or within, the adipose cell was detected, nor could any cell organelle be demonstrated to be visibly related to lipid synthesis and/or deposition.
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Adipocytes are enmeshed in a dense network of ECM.
“Adipocytes are enmeshed in a dense network of ECM (100, 101)”
From Adipose tissue remodeling and obesity (2011) · cites this paper as [100]
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- Authors
- Napolitano, L.
- Affiliations
- University of Pittsburgh (US)
- Corresponding author
- Napolitano, Leonard
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 1963-09-01
- Volume
- 18
- Issue
- 3
- Pages
- 663-679
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2106313
- OpenAlex
- W1990614859
- Cited by
- 421
- References
- 14
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 6 · 2023: 5 · 2022: 4 · 2021: 4 · 2020: 1 · 2019: 5 · 2018: 10 · 2017: 5 · 2016: 10 · 2015: 8 · 2014: 10 · 2013: 17 · 2012: 12
- FWCI
- 58.72
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- The Journal of Cell Biology (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Funders
- U.S. Public Health Service
- Topics
- Health Sciences › Medicine › Physiology › Adipose Tissue and Metabolism
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biochemistry › Lipid metabolism and biosynthesis
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Peroxisome Proliferator-Activated Receptors
- Keywords
- white adipocyte differentiation, fibroblasts, adipocyte development, endoplasmic reticulum, mitochondria, glycogen, pinocytosis, brown adipocytes, lipid accumulation, lipogenesis
- MeSH terms
- Adipose Tissue, Animals, Capillaries, Cell Differentiation, Cell Membrane, Cytoplasm, Electrons, Endoplasmic Reticulum, Fibroblasts, Glycogen, Microscopy, Microscopy, Electron, Mitochondria, Pinocytosis, Rats
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