The DOI matches its Crossref record; 32 of the 32 references it cites were found in a registry or an index.
Junctional adhesion molecule (JAM) binds to PAR-3
Open accessAbstract
At tight junctions (TJs), claudins with four transmembrane domains are incorporated into TJ strands. Junctional adhesion molecule (JAM), which belongs to the immunoglobulin superfamily, is also localized at TJs, but it remains unclear how JAM is integrated into TJs. Immunoreplica electron microscopy revealed that JAM showed an intimate spatial relationship with TJ strands in epithelial cells. In L fibroblasts expressing exogenous JAM, JAM was concentrated at cell–cell adhesion sites, where there were no strand-like structures, but rather characteristic membrane domains free of intramembranous particles were detected. These domains were specifically labeled with anti-JAM polyclonal antibody, suggesting that JAM forms planar aggregates through their lateral self-association. Immunofluorescence microscopy and in vitro binding assays revealed that ZO-1 directly binds to the COOH termini of claudins and JAM at its PDZ1 and PDZ3 domains, respectively. Furthermore, another PDZ-containing polarity-related protein, PAR-3, was directly bound to the COOH terminus of JAM, but not to that of claudins. These findings led to a molecular architectural model for TJs: small aggregates of JAM are tethered to claudin-based strands through ZO-1, and these JAM aggregates recruit PAR-3 to TJs. We also discuss the importance of this model from the perspective of the general molecular mechanisms behind the recruitment of PAR proteins to plasma membranes.
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Cites
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“Since these JAM molecules would be free of ZO-1, they could recruit PAR-3 and then its binding proteins, such as atypical PKC, PAR-6, and Cdc42, to TJs (Joberty et al., 2000; Lin et al., 2000; Qiu et al., 2000; Suzuki et al., 2001).”
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Self-citationRegulation of Tight Junction Permeability by Intestinal Bacteria and Dietary Components1,2
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Metadata
- Authors
- Itoh, M., Sasaki, H., Furuse, M., Ozaki, H., Kita, T., & Tsukita, S.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2001-08-06
- Volume
- 154
- Issue
- 3
- Pages
- 491-498
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2024349959
- Cited by
- 386
- References
- 32
- FWCI
- 15.91
- Type
- journal article
- Language
- en
- Open access
- green
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