The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Molecular requirements for actin-based lamella formation in Drosophila S2 cells
Open accessAbstract
Cell migration occurs through the protrusion of the actin-enriched lamella. Here, we investigated the effects of RNAi depletion of ∼90 proteins implicated in actin function on lamella formation in Drosophila S2 cells. Similar to in vitro reconstitution studies of actin-based Listeria movement, we find that lamellae formation requires a relatively small set of proteins that participate in actin nucleation (Arp2/3 and SCAR), barbed end capping (capping protein), filament depolymerization (cofilin and Aip1), and actin monomer binding (profilin and cyclase-associated protein). Lamellae are initiated by parallel and partially redundant signaling pathways involving Rac GTPases and the adaptor protein Nck, which stimulate SCAR, an Arp2/3 activator. We also show that RNAi of three proteins (kette, Abi, and Sra-1) known to copurify with and inhibit SCAR in vitro leads to SCAR degradation, revealing a novel function of this protein complex in SCAR stability. Our results have identified an essential set of proteins involved in actin dynamics during lamella formation in Drosophila S2 cells.
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Cites
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Reconstitution of actin-based motility of Listeria and Shigella using pure proteins
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The lamellipodium: where motility begins
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Antagonism between Ena/VASP Proteins and Actin Filament Capping Regulates Fibroblast Motility
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Cited by
The WASP-WAVE protein network: Connecting the membrane to the cytoskeleton.
Takenawa, T., & Suetsugu, S. (2007) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2069 · Cited by 947
Lamellipodial Versus Filopodial Mode of the Actin Nanomachinery
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Sra‐1 and Nap1 link Rac to actin assembly driving lamellipodia formation
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Metadata
- Authors
- Rogers, S. L., Wiedemann, U., Stuurman, N., & Vale, R. D.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2003-09-15
- Volume
- 162
- Issue
- 6
- Pages
- 1079-1088
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2101173100
- Cited by
- 401
- References
- 37
- FWCI
- 12.62
- Type
- journal article
- Language
- en
- Open access
- green
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