The DOI matches its Crossref record; 29 of the 29 references it cites were found in a registry or an index.
Mechanisms for segregating T cell receptor and adhesion molecules during immunological synapse formation in Jurkat T cells
Open accessAbstract
T cells interacting with antigen-presenting cells (APCs) form an “immunological synapse” (IS), a bull's-eye pattern composed of a central supramolecular activation cluster enriched with T cell receptors (TCRs) surrounded by a ring of adhesion molecules (a peripheral supramolecular activation cluster). The mechanism responsible for segregating TCR and adhesion molecules remains poorly understood. Here, we show that immortalized Jurkat T cells interacting with a planar lipid bilayer (mimicking an APC) will form an IS, thereby providing an accessible model system for studying the cell biological processes underlying IS formation. We found that an actin-dependent process caused TCR and adhesion proteins to cluster at the cell periphery, but these molecules appeared to segregate from one another at the earliest stages of microdomain formation. The TCR and adhesion microdomains attached to actin and were carried centripetally by retrograde flow. However, only the TCR microdomains penetrated into the actin-depleted cell center, whereas the adhesion microdomains appeared to be unstable without an underlying actin cytoskeleton. Our results reveal that TCR and adhesion molecules spatially partition from one another well before the formation of a mature IS and that differential actin interactions help to shape and maintain the final bull's-eye pattern of the IS.
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The Immunological Synapse: A Molecular Machine Controlling T Cell Activation
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Self-citationThree-dimensional segregation of supramolecular activation clusters in T cells
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Self-citationTwo Distinct Actin Networks Drive the Protrusion of Migrating Cells
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Self-citationDustin, M. L., Olszowy, M. W., Holdorf, A. D., Li, J., Bromley, S., Desai, N., Widder, P., Rosenberger, F., van der Merwe, P. A., Allen, P. M., et al. (1998) · Cell · doi:10.1016/s0092-8674(00)81608-6 · Cited by 723
Self-citationYokosuka, T., Sakata-Sogawa, K., Kobayashi, W., Hiroshima, M., Hashimoto-Tane, A., Tokunaga, M., Dustin, M. L., & Saito, T. (2005) · Nature Immunology · doi:10.1038/ni1272 · Cited by 698
Self-citationT Cell Receptor Signaling Precedes Immunological Synapse Formation
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Self-citationT cell receptor ligation induces the formation of dynamically regulated signaling assemblies
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Metadata
- Authors
- Kaizuka, Y., Douglass, A. D., Varma, R., Dustin, M. L., & Vale, R. D.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 2007-12-18
- Volume
- 104
- Issue
- 51
- Pages
- 20296-20301
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2083483039
- Cited by
- 384
- References
- 29
- FWCI
- 8.99
- Type
- journal article
- Language
- en
- Open access
- green
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- Kaizuka, Y., Douglass, A. D., Varma, R., Dustin, M. L., & Vale, R. D. (2007). Mechanisms for segregating T cell receptor and adhesion molecules during immunological synapse formation in Jurkat T cells. Proceedings of the National Academy of Sciences. https://doi.org/10.1073/pnas.0710258105
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