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Cited by 498Genomics and Chromatin DynamicsChromosomal and Genetic VariationsNuclear Structure and Function

Mouse centric and pericentric satellite repeats form distinct functional heterochromatin

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Guenatri, Mounia, Bailly, Delphine, Maison, Christèle, Almouzni, Geneviève·2004·The Journal of Cell Biology
DOI 10.1083/jcb.200403109PMC PMC2172221
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Abstract

Heterochromatin is thought to play a critical role for centromeric function. However, the respective contributions of the distinct repetitive sequences found in these regions, such as minor and major satellites in the mouse, have remained largely unsolved. We show that these centric and pericentric repeats on the chromosomes have distinct heterochromatic characteristics in the nucleus. Major satellites from different chromosomes form clusters associated with heterochromatin protein 1α, whereas minor satellites are individual entities associated with centromeric proteins. Both regions contain methylated histone H3 (Me-K9 H3) but show different micrococcal nuclease sensitivities. A dinucleosome repeating unit is found specifically associated with major satellites. These domains replicate asynchronously, and chromatid cohesion is sustained for a longer time in major satellites compared with minor satellites. Such prolonged cohesion in major satellites is lost in the absence of Suv39h histone methyltransferases. Thus, we define functionally independent centromeric subdomains, which spatio-temporal isolation is proposed to be important for centromeric cohesion and dissociation during chromosome segregation.

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  • Within the nucleoplasm, ESRP2 displayed a punctate staining pattern that concentrated near the euchromatic and away from the heterochromatic centers.

    “We further noticed that within the nucleoplasm, ESRP2 displayed a punctate staining pattern that concentrated near the euchromatic and away from the heterochromatic centers (Guenatri et al. 2004).”

    From ESRP2–microRNA-122 axis promotes the postnatal onset of liver polyploidization and maturation (2025) · cites this paper as Guenatri et al. 2004

    The Introduction identifies mouse chromocenters as centromeric heterochromatin clusters, and the Results describe DAPI-dense heterochromatin clusters in interphase nuclei. These passages support the reference to heterochromatic centers, but the paper does not report ESRP2 staining or its position relative to euchromatin; the citing authors present that pattern as their own observation. (LLM, conf=0.84)

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Authors
Guenatri, M., Bailly, D., Maison, C., & Almouzni, G.
Affiliations
  • Centre National de la Recherche Scientifique (FR)
  • Institut Curie/Research section, UMR218 du Centre National pour la Recherche Scientifique, 75248 Paris Cedex 05, France
Venue
The Journal of Cell Biology
Publisher
Rockefeller University Press
Published
2004-08-09
Volume
166
Issue
4
Pages
493-505
ISSN
  • 0021-9525 (ISSN-L)
  • 1540-8140
DOI
10.1083/jcb.200403109
PMC
PMC2172221
OpenAlex
W2160352834
Cited by
498
References
1
Citations by year
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FWCI
7.00
Type
journal article
Language
en
Open access
Licence
cc-by-nc-sa
Locations
  • The Journal of Cell Biology (publishedVersion)
  • PubMed Central (publishedVersion, cc-by-nc-sa)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Chromatin Dynamics
  • Life Sciences › Agricultural and Biological Sciences › Plant Science › Chromosomal and Genetic Variations
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Nuclear Structure and Function
Keywords
heterochromatin, centromeric repeats, major satellite DNA, pericentromeric heterochromatin, centromere, histone H3 methylation, dinucleosome, centromeric cohesion, Suv39h, chromosome segregation, mouse chromosomes
MeSH terms
Animals, Bromodeoxyuridine, Cell Line, Cell Nucleus, Centromere, Chromatin, Chromosomes, DNA, Satellite, Multigene Family, Heterochromatin, Histones, Image Processing, Computer-Assisted, Interphase, Methyltransferases, Micrococcal Nuclease, Mutation, Plasmids, Repetitive Sequences, Nucleic Acid, Repressor Proteins, Sister Chromatid Exchange, Time Factors, S Phase, In Situ Hybridization, Fluorescence, Kinetochores, Microsatellite Repeats, NIH 3T3 Cells, Mice

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