The DOI matches its Crossref record; 5 of the 5 references it cites were found in a registry or an index.
The Rac activator Tiam1 controls tight junction biogenesis in keratinocytes through binding to and activation of the Par polarity complex
Open accessAbstract
The GTPases Rac and Cdc42 play a pivotal role in the establishment of cell polarity by stimulating biogenesis of tight junctions (TJs). In this study, we show that the Rac-specific guanine nucleotide exchange factor Tiam1 (T-lymphoma invasion and metastasis) controls the cell polarity of epidermal keratinocytes. Similar to wild-type (WT) keratinocytes, Tiam1-deficient cells establish primordial E-cadherin–based adhesions, but subsequent junction maturation and membrane sealing are severely impaired. Tiam1 and V12Rac1 can rescue the TJ maturation defect in Tiam1-deficient cells, indicating that this defect is the result of impaired Tiam1–Rac signaling. Tiam1 interacts with Par3 and aPKCζ, which are two components of the conserved Par3–Par6–aPKC polarity complex, and triggers biogenesis of the TJ through the activation of Rac and aPKCζ, which is independent of Cdc42. Rac is activated upon the formation of primordial adhesions (PAs) in WT but not in Tiam1-deficient cells. Our data indicate that Tiam1-mediated activation of Rac in PAs controls TJ biogenesis and polarity in epithelial cells by association with and activation of the Par3–Par6–aPKC polarity complex.
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Cites
A mammalian PAR-3–PAR-6 complex implicated in Cdc42/Rac1 and aPKC signalling and cell polarity
Lin, D., Edwards, A. S., Fawcett, J. P., Mbamalu, G., Scott, J. D., & Pawson, T. (2000) · Nature Cell Biology · doi:10.1038/35019582 · Cited by 743
E‐cadherin is essential for in vivo epidermal barrier function by regulating tight junctions
Tunggal, J. A., Helfrich, I., Schmitz, A., Schwarz, H., Günzel, D., Fromm, M., Kemler, R., Krieg, T., & Niessen, C. M. (2005) · The EMBO Journal · doi:10.1038/sj.emboj.7600605 · Cited by 491 · cited 2×
“These conclusions are consistent with studies that show the activity of Cdc42 is dispensable for cell polarization in epithelial MDCK cells and epidermal keratinocytes (Gao et al., 2002; Tunggal et al., 2005), although another study has implicated Cdc42 in TJ biogenesis (Fukuhara et al., 2003).”
ResultThe sequential activity of the GTPases Rap1B and Cdc42 determines neuronal polarity.
Schwamborn, J. C., & Püschel, A. W. (2004) · Nature Neuroscience · doi:10.1038/nn1295 · Cited by 385
Structural and Functional Regulation of Tight Junctions by RhoA and Rac1 Small GTPases
Jou, T.-S., Schneeberger, E. E., & James Nelson, W. (1998) · The Journal of Cell Biology · doi:10.1083/jcb.142.1.101 · Cited by 354
Suzuki, A., Ishiyama, C., Hashiba, K., Shimizu, M., Ebnet, K., & Ohno, S. (2002) · Journal of Cell Science · doi:10.1242/jcs.00032 · Cited by 251
Cited by
Cancer Invasion and the Microenvironment: Plasticity and Reciprocity
Friedl, P., & Alexander, S. (2011) · Cell · doi:10.1016/j.cell.2011.11.016 · Cited by 1,940
Adherens junctions: from molecules to morphogenesis
Harris, T. J. C., & Tepass, U. (2010) · Nature Reviews Molecular Cell Biology · doi:10.1038/nrm2927 · Cited by 990
Tight Junctions and Cell Polarity
Shin, K., Fogg, V. C., & Margolis, B. (2006) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev.cellbio.22.010305.104219 · Cited by 745
Martin-Belmonte, F., Gassama, A., Datta, A., Yu, W., Rescher, U., Gerke, V., & Mostov, K. (2007) · Cell · doi:10.1016/j.cell.2006.11.051 · Cited by 699
Coordinated protein sorting, targeting and distribution in polarized cells
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Metadata
- Authors
- Mertens, A. E. E., Rygiel, T. P., Olivo, C., van der Kammen, R., & Collard, J. G.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2005-09-26
- Volume
- 170
- Issue
- 7
- Pages
- 1029-1037
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2058170014
- Cited by
- 238
- References
- 5
- FWCI
- 7.72
- Type
- journal article
- Language
- en
- Open access
- green
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- Mertens, A. E. E., Rygiel, T. P., Olivo, C., van der Kammen, R., & Collard, J. G. (2005). The Rac activator Tiam1 controls tight junction biogenesis in keratinocytes through binding to and activation of the Par polarity complex. The Journal of Cell Biology. https://doi.org/10.1083/jcb.200502129
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