The DOI matches its Crossref record; 48 of the 48 references it cites were found in a registry or an index.
Activation of endosomal dynein motors by stepwise assembly of Rab7–RILP–p150Glued, ORP1L, and the receptor βlll spectrin
Open accessAbstract
The small GTPase Rab7 controls late endocytic transport by the minus end–directed motor protein complex dynein–dynactin, but how it does this is unclear. Rab7-interacting lysosomal protein (RILP) and oxysterol-binding protein–related protein 1L (ORP1L) are two effectors of Rab7. We show that GTP-bound Rab7 simultaneously binds RILP and ORP1L to form a RILP–Rab7–ORP1L complex. RILP interacts directly with the C-terminal 25-kD region of the dynactin projecting arm p150Glued, which is required for dynein motor recruitment to late endocytic compartments (LEs). Still, p150Glued recruitment by Rab7–RILP does not suffice to induce dynein-driven minus-end transport of LEs. ORP1L, as well as βIII spectrin, which is the general receptor for dynactin on vesicles, are essential for dynein motor activity. Our results illustrate that the assembly of microtubule motors on endosomes involves a cascade of linked events. First, Rab7 recruits two effectors, RILP and ORP1L, to form a tripartite complex. Next, RILP directly binds to the p150Glued dynactin subunit to recruit the dynein motor. Finally, the specific dynein motor receptor Rab7–RILP is transferred by ORP1L to βIII spectrin. Dynein will initiate translocation of late endosomes to microtubule minus ends only after interacting with βIII spectrin, which requires the activities of Rab7–RILP and ORP1L.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“Retrograde movement is regulated by Rab7 and its effector RILP. RILP interacts with the p150-glued subunit of dynactin, thus recruiting the minus-end-directed microtubule motor dynein to lysosomes23–25.”
From TFEB regulates lysosomal positioning by modulating TMEM55B expression and JIP4 recruitment to lysosomes (2017) · cites this paper as [24]
Experimental — accuracy not yet measuredIn the citing paper
“Downstream effector proteins of Rab7A include the Rab-interacting lysosomal protein (RILP), which links the phagosomal membrane to a motor complex consisting of dynein and dynactin through stepwise recruitment of p150Glued, ORP1L, and the receptor βlll spectrin (33, 34).”
From Macrophage defense mechanisms against intracellular bacteria (2015) · cites this paper as [33]
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Zwischenmolekulare Energiewanderung und Fluoreszenz
Förster, T. (1948) · Annalen der Physik · doi:10.1002/andp.19484370105 · Cited by 8,285
Protein production by auto-induction in high-density shaking cultures
Studier, F. W. (2005) · Protein Expression and Purification · doi:10.1016/j.pep.2005.01.016 · Cited by 5,949
Predicting coiled coils from protein sequences.
Lupas, A., Van Dyke, M., & Stock, J. (1991) · Science · doi:10.1126/science.252.5009.1162 · Cited by 3,953
The Molecular Motor Toolbox for Intracellular Transport
Vale, R. D. (2003) · Cell · doi:10.1016/s0092-8674(03)00111-9 · Cited by 1,982
Kinesin and Dynein Superfamily Proteins and the Mechanism of Organelle Transport
Hirokawa, N. (1998) · Science · doi:10.1126/science.279.5350.519 · Cited by 1,689
Jordens, I., Fernandez-Borja, M., Marsman, M., Dusseljee, S., Janssen, L., Calafat, J., Janssen, H., Wubbolts, R., & Neefjes, J. (2001) · Current Biology · doi:10.1016/s0960-9822(01)00531-0 · Cited by 811
Self-citationImaging protein molecules using FRET and FLIM microscopy
Wallrabe, H. K., & Periasamy, A. (2004) · Current Opinion in Biotechnology · doi:10.1016/j.copbio.2004.12.002 · Cited by 728
Fluorescence lifetime imaging microscopy: spatial resolution of biochemical processes in the cell
Bastiaens, P. I. H. (1999) · Trends in Cell Biology · doi:10.1016/s0962-8924(98)01410-x · Cited by 723
Burkhardt, J. K., Echeverri, C. J., Nilsson, T., & Vallee, R. B. (1997) · The Journal of Cell Biology · doi:10.1083/jcb.139.2.469 · Cited by 667
Dynactin increases the processivity of the cytoplasmic dynein motor
King, S. J., & Schroer, T. A. (1999) · Nature Cell Biology · doi:10.1038/71338 · Cited by 661
Flagged first, then by citations
Research works printed but not linked: 1
- 1948, Annalen Physik., 6, 55
Cited by
Sorted by citations
Items 501–506 of 506
Vilela, F., Chenon, M., Campanacci, V., Alik, A., Velours, C., Andreani, J., Llinas, P., & Ménétrey, J. (2026) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2026.113355 · Cited by 2
Rabs in the driving seat: Coordinating vesicular traffic and membrane contact site dynamics
Phogat, Y., Paul, S., & Sharma, M. (2026) · Current Opinion in Cell Biology · doi:10.1016/j.ceb.2026.102677
Sarring, C., Stoute, J., Armache, J., Yennawar, N. H., & Thomas, C. M. (2026) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2026.113388
Courtney, S. C. (2022) · Digital Archive @ GSU · doi:10.57709/2374518
TMEM55B downregulation promotes neurodegeneration in Alzheimer’s disease models
Roy, B., Contu, V. R., Jeziorek, M., Friedman, W. J., & Wong, C.-O. (2026) · doi:10.64898/2026.10.21.760809
Metadata
- Authors
- Johansson, M., Rocha, N., Zwart, W., Jordens, I., Janssen, L., Kuijl, C., Olkkonen, V. M., & Neefjes, J.
- Affiliations
- 1Department of Molecular Medicine, National Public Health Institute, Biomedicum, FI-00251 Helsinki, Finland
- The Netherlands Cancer Institute (NL)
- Corresponding author
- Neefjes, Jacques
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2007-02-05
- Volume
- 176
- Issue
- 4
- Pages
- 459-471
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- PMC
- PMC2063981
- OpenAlex
- W2153698618
- Cited by
- 505
- References
- 48
- Citations by year
- 2026: 6 · 2025: 21 · 2024: 19 · 2023: 21 · 2022: 32 · 2021: 26 · 2020: 30 · 2019: 29 · 2018: 36 · 2017: 34 · 2016: 32 · 2015: 24 · 2014: 32 · 2013: 23 · 2012: 35
- FWCI
- 10.76
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Licence
- cc-by-nc-sa
- Locations
- The Journal of Cell Biology (publishedVersion)
- PubMed Central (publishedVersion, cc-by-nc-sa)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Cellular transport and secretion
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Microtubule and mitosis dynamics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Cell Biology › Autophagy in Disease and Therapy
- Keywords
- Rab7, RILP, p150Glued, dynactin, dynein motor, late endosomes, endosomal transport, microtubule motor proteins, GTPase effectors
- MeSH terms
- Dynactin Complex, rab7 GTP-Binding Proteins, Oxysterol Binding Proteins, Carrier Proteins, Dyneins, Endocytosis, HeLa Cells, Humans, Microtubule-Associated Proteins, Microtubules, Models, Molecular, Receptors, Steroid, Endosomes, Spectrin, Signal Transduction, Molecular Motor Proteins, rab GTP-Binding Proteins, Protein Transport, Macromolecular Substances, Adaptor Proteins, Signal Transducing
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
