The DOI matches its Crossref record; 44 of the 44 references it cites were found in a registry or an index.
In macrophages, HIV-1 assembles into an intracellular plasma membrane domain containing the tetraspanins CD81, CD9, and CD53
Open accessAbstract
In macrophages, HIV-1 has been shown to bud into intracellular structures that contain the late endosome marker CD63. We show that these organelles are not endosomes, but an internally sequestered plasma membrane domain. Using immunofluorescence microscopy and immunoelectron microscopy, we find that HIV-1 buds into a compartment that contains the tetraspanins CD81, CD9, and CD53. On uninfected macrophages, these proteins are seen at the cell surface and in intracellular vacuole-like structures with a complex content of vesicles and interconnected membranes that lack endosome markers, including CD63. Significantly, these structures are accessible to small tracers (horseradish peroxidase or ruthenium red) applied to cells at 4°C, indicating that they are connected to the cell surface. HIV assembles on, and accumulates within, these intracellular compartments. Furthermore, CD63 is recruited to the virus-containing structures and incorporated into virions. These results indicate that, in macrophages, HIV-1 exploits a previously undescribed intracellular plasma membrane domain to assemble infectious particles.
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Cited by
Pegtel, D. M., & Gould, S. J. (2019) · Annual Review of Biochemistry · doi:10.1146/annurev-biochem-013118-111902 · Cited by 2,682
HIV-1 assembly, budding, and maturation
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Autophagy pathway intersects with HIV-1 biosynthesis and regulates viral yields in macrophages
Kyei, G. B., Dinkins, C., Davis, A. S., Roberts, E., Singh, S. B., Dong, C., Wu, L., Kominami, E., Ueno, T., Yamamoto, A., et al. (2009) · Journal of Cell Biology · doi:10.1083/jcb.200903070 · Cited by 509
The function and clinical application of extracellular vesicles in innate immune regulation
Zhou, X., Xie, F., Wang, L., Zhang, L., Zhang, S., Fang, M., & Zhou, F. (2020) · Cellular & Molecular Immunology · doi:10.1038/s41423-020-0391-1 · Cited by 294
Chan, R., Uchil, P. D., Jin, J., Shui, G., Ott, D. E., Mothes, W., & Wenk, M. R. (2008) · Journal of Virology · doi:10.1128/jvi.00981-08 · Cited by 282
Metadata
- Authors
- Deneka, M., Pelchen-Matthews, A., Byland, R., Ruiz-Mateos, E., & Marsh, M.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2007-04-16
- Volume
- 177
- Issue
- 2
- Pages
- 329-341
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2159507231
- Cited by
- 339
- References
- 44
- FWCI
- 11.54
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Deneka, M., Pelchen-Matthews, A., Byland, R., Ruiz-Mateos, E., & Marsh, M. (2007). In macrophages, HIV-1 assembles into an intracellular plasma membrane domain containing the tetraspanins CD81, CD9, and CD53. The Journal of Cell Biology. https://doi.org/10.1083/jcb.200609050
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