The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
The structural basis of actin filament branching by the Arp2/3 complex
Open accessAbstract
The actin-related protein 2/3 (Arp2/3) complex mediates the formation of branched actin filaments at the leading edge of motile cells and in the comet tails moving certain intracellular pathogens. Crystal structures of the Arp2/3 complex are available, but the architecture of the junction formed by the Arp2/3 complex at the base of the branch was not known. In this study, we use electron tomography to reconstruct the branch junction with sufficient resolution to show how the Arp2/3 complex interacts with the mother filament. Our analysis reveals conformational changes in both the mother filament and Arp2/3 complex upon branch formation. The Arp2 and Arp3 subunits reorganize into a dimer, providing a short-pitch template for elongation of the daughter filament. Two subunits of the mother filament undergo conformational changes that increase stability of the branch. These data provide a rationale for why branch formation requires cooperative interactions among the Arp2/3 complex, nucleation-promoting factors, an actin monomer, and the mother filament.
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Cites
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Self-citationCrystal Structure of Arp2/3 Complex
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Self-citationThe Crystal Structure of Uncomplexed Actin in the ADP State
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Cited by
A nucleator arms race: cellular control of actin assembly
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Self-citationActin Dynamics at the Leading Edge: From Simple Machinery to Complex Networks
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Molecular dynamics simulations of large macromolecular complexes
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Metadata
- Authors
- Rouiller, I., Xu, X.-P., Amann, K. J., Egile, C., Nickell, S., Nicastro, D., Li, R., Pollard, T. D., Volkmann, N., & Hanein, D.
- Venue
- The Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2008-03-03
- Volume
- 180
- Issue
- 5
- Pages
- 887-895
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W1999619602
- Cited by
- 321
- References
- 36
- FWCI
- 9.07
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Rouiller, I., Xu, X.-P., Amann, K. J., Egile, C., Nickell, S., Nicastro, D., Li, R., Pollard, T. D., Volkmann, N., & Hanein, D. (2008). The structural basis of actin filament branching by the Arp2/3 complex. The Journal of Cell Biology. https://doi.org/10.1083/jcb.200709092
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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