The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Exocytosis of acid sphingomyelinase by wounded cells promotes endocytosis and plasma membrane repair
Open accessAbstract
Rapid plasma membrane resealing is essential for cellular survival. Earlier studies showed that plasma membrane repair requires Ca2+-dependent exocytosis of lysosomes and a rapid form of endocytosis that removes membrane lesions. However, the functional relationship between lysosomal exocytosis and the rapid endocytosis that follows membrane injury is unknown. In this study, we show that the lysosomal enzyme acid sphingomyelinase (ASM) is released extracellularly when cells are wounded in the presence of Ca2+. ASM-deficient cells, including human cells from Niemann-Pick type A (NPA) patients, undergo lysosomal exocytosis after wounding but are defective in injury-dependent endocytosis and plasma membrane repair. Exogenously added recombinant human ASM restores endocytosis and resealing in ASM-depleted cells, suggesting that conversion of plasma membrane sphingomyelin to ceramide by this lysosomal enzyme promotes lesion internalization. These findings reveal a molecular mechanism for restoration of plasma membrane integrity through exocytosis of lysosomes and identify defective plasma membrane repair as a possible component of the severe pathology observed in NPA patients.
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Cites
Impaired sphingomyelinase activity and epidermal differentiation in atopic dermatitis
Jensen, J.-M., Fölster-Holst, R., Baranowsky, A., Schunck, M., Winoto-Morbach, S., Neumann, C., Schütze, S., & Proksch, E. (2004) · Journal of Investigative Dermatology · doi:10.1111/j.0022-202x.2004.22621.x · Cited by 309
Cited by
The lysosome: from waste bag to potential therapeutic target
Appelqvist, H., Wäster, P., Kågedal, K., & Öllinger, K. (2013) · Journal of Molecular Cell Biology · doi:10.1093/jmcb/mjt022 · Cited by 772
Lysosomal Exocytosis: The Extracellular Role of an Intracellular Organelle
Tancini, B., Buratta, S., Delo, F., Sagini, K., Chiaradia, E., Pellegrino, R. M., Emiliani, C., & Urbanelli, L. (2020) · Membranes · doi:10.3390/membranes10120406 · Cited by 144
Spoiled for Choice: Diverse Endocytic Pathways Function at the Cell Surface
Thottacherry, J. J., Sathe, M., Prabhakara, C., & Mayor, S. (2019) · Annual Review of Cell and Developmental Biology · doi:10.1146/annurev-cellbio-100617-062710 · Cited by 122
Regulation of Lytic and Non-Lytic Functions of Gasdermin Pores
Rühl, S., & Broz, P. (2022) · Journal of Molecular Biology · doi:10.1016/j.jmb.2021.167246 · Cited by 74
The endolysosomal system in conventional and unconventional protein secretion
Néel, E., Chiritoiu-Butnaru, M., Fargues, W., Denus, M., Colladant, M., Filaquier, A., Stewart, S. E., Lehmann, S., Zurzolo, C., Rubinsztein, D. C., et al. (2024) · Journal of Cell Biology · doi:10.1083/jcb.202404152 · Cited by 50
Metadata
- Authors
- Tam, C., Idone, V., Devlin, C., Fernandes, M. C., Flannery, A., He, X., Schuchman, E., Tabas, I., & Andrews, N. W.
- Venue
- Journal of Cell Biology
- Publisher
- Rockefeller University Press
- Published
- 2010-06-07
- Volume
- 189
- Issue
- 6
- Pages
- 1027-1038
- ISSN
- 0021-9525 (ISSN-L)
- 1540-8140
- OpenAlex
- W2154504541
- Cited by
- 364
- References
- 1
- FWCI
- 8.10
- Type
- journal article
- Language
- en
- Open access
- hybrid
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- Tam, C., Idone, V., Devlin, C., Fernandes, M. C., Flannery, A., He, X., Schuchman, E., Tabas, I., & Andrews, N. W. (2010). Exocytosis of acid sphingomyelinase by wounded cells promotes endocytosis and plasma membrane repair. Journal of Cell Biology. https://doi.org/10.1083/jcb.201003053
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